Evidence of anoxic methane oxidation coupled to denitrification

Islas-Lima, S; Thalasso, F.; Gómez-Hernández, Jorge

doi:10.1016/j.watres.2003.08.024

Cited by 127 publications

(68 citation statements)

References 19 publications

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“…Raghoebarsing et al, (2006) purified and identified a microbial consortium formed by two microorganisms: an unidentified bacterium and an archaea similar to the marine archaea capable of oxidizing methane to carbon dioxide and favoring the denitrification from nitrate and nitrite under anaerobic conditions. The present results as well as those obtained by Islas-Lima et al, (2004) and Raghoebarsing et al, (2006) suggested that the oxidation of methane in the presence of nitrate or nitrite was possible. Apparently, the microbes responsible for the aerobic methane oxidation were capable of adaptation to anoxic conditions.…”

Section: Discussionsupporting

confidence: 88%

“…However, little is known about anoxic nitrogen compound reduction with methane as an electron donor or the organisms involved in the process. Islas-Lima et al, (2004) studied the dissimilative nitrate reduction in the presence of methane under anoxic conditions. The highest denitrification rate obtained was 0.25 gNO 3 --N g -1 VSS d -1 for partial pressures of methane equal or higher than 8.8 kPa.…”

Section: Introductionmentioning

confidence: 99%

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Denitrification coupled with methane anoxic oxidation and microbial community involved identification

Cuba

Duarte

Saavedra

et al. 2011

Braz. arch. biol. technol.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Denitrification coupled with methane anoxic oxidation and microbial community involved identification

Cuba

Duarte

Saavedra

et al. 2011

Braz. arch. biol. technol.

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“…Panganiban et al, 1979;Smith et al, 1993;Eller et al, 2005), two landfills (Bjerg et al, 1995;Grossman et al, 2002), anoxic waste slurries (Malek and Weismann, 1988), a contaminated aquifer (Smith et al, 1991), and flooded-rice paddies (Miura et al, 1992;Murase and Kimura, 1994b), but much of the evidence is anecdotal in nature and strong evidence for AOM in freshwater systems has been limited. Work by Islas-Lima et al (2004) and Raghoebarsing et al (2006) has demonstrated that AOM in some freshwater systems is linked to denitrification and dentrifying bacteria, which provides an energetically favorable alternative to marine AOM linked to SR. Further work has suggested that AOM can be carried out by denitrifying bacteria in the absence of an archeal consortium (Ettwig et al, 2008) and that this process might be linked to nitrite (NO − 2 ) reduction and the production of oxygen (O 2 ) as an electron acceptor ; hence, implying aerobic metabolism under anoxic conditions.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

2011

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Abstract.Despite a large body of literature on microbial anaerobic oxidation of methane (AOM) in marine sediments and saline waters and its importance to the global methane (CH 4 ) cycle, until recently little work has addressed the potential occurrence and importance of AOM in non-marine systems. This is particularly true for peatlands, which represent both a massive sink for atmospheric CO 2 and a significant source of atmospheric CH 4 . Our knowledge of this process in peatlands is inherently limited by the methods used to study CH 4 dynamics in soil and sediment and the assumption that there are no anaerobic sinks for CH 4 in these systems. Studies suggest that AOM is CH 4 -limited and difficult to detect in potential CH 4 production assays against a background of CH 4 production. In situ rates also might be elusive due to background rates of aerobic CH 4 oxidation and the difficulty in separating net and gross process rates. Conclusive evidence for the electron acceptor in this process has not been presented. Nitrate and sulfate are both plausible and favorable electron acceptors, as seen in other systems, but there exist theoretical issues related to the availability of these ions in peatlands and only circumstantial evidence suggests that these pathways are important. Iron cycling is important in many wetland systems, but recent evidence does not support the notion of CH 4 oxidation via dissimilatory Fe(III) reduction or a CH 4 oxidizing archaea in consortium with an Fe(III) reducer. Calculations based on published rates demonstrate that AOM might be a significant and underappreciated constraint on the global CH 4 cycle, although much about the process is unknown, in vitro rates may not relate well to in situ rates, and projections based on those rates are fraught with uncertainty. We suggest electron transfer mechanisms, C flow and pathways, and quantifying in situ peatland AOM rates as the highest priority topics for future research.

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“…In the 1970s, Mason 8 discredited earlier studies that pure cultures of methanotrophic bacteria were able to denitrify using methane as the sole carbon and energy source. Instead, it was established experimentally that methanotrophs can oxidize methane aerobically to methanol or acetate at low oxygen concentrations, and that the methanol or acetate can subsequently be used to drive denitrification [23][24][25][26] . Thus, the reaction presented here defines a new microbial guild with a potential contribution to biogeochemical cycling that has so far been overlooked.…”

mentioning

confidence: 99%