Evidence of Antibodies against the West Nile Virus and the Usutu Virus in Dogs and Horses from the Southeast of France

Laidoudi, Younes; Durand, Guillaume; Watier-Grillot, Stéphanie; Dessimoulie, Anne-Sophie; Labarde, Claire; Normand, T.; Andréo, Virginie; Guérin, Patrick M.; Grard, Gilda; Davoust, Bernard

doi:10.1155/2023/8779723

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…albopictus are not autochthonous [36,37]. However, sporadic autochthonous cases of DENV-1 in Italy, Croatia, and southern France [38][39][40][41], as well as CHIKV outbreaks in Italy and France [40][41][42][43][44], and WNV cases in Italy, Germany, the Netherlands, France [45][46][47][48][49][50][51], have been reported. The frequency of Ae.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection, Characterization, and Temperature-Dependence of Wolbachia Infections in Field Populations of <em>Aedes albopictus</em> (Diptera: Culicidae) Mosquitoes in Greece

Misailidis,

Kotsiou,

Moulistanos

et al. 2023

Preprint

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We investigated the prevalence and genetic diversity of Wolbachia pipientis strains in Aedes albopictus populations in Greece. Using a combination of PCR and Sanger sequencing techniques, we genotyped Wolbachia strains in 105 mosquitoes collected across eight different prefectures in 2021. We found a high prevalence of Wolbachia in both male (90%) and female (97%) mosquitoes. Among the infected samples, 84% had double infections with both wAlbA and wAlbB strains, while 16% had infections with only wAlbB. Our comparison of the Multi-Locus Sequence Typing (MLST) profile, employing gatB-coxA-hcpA-ftsZ-fbpA genotyping, revealed a single MLST profile for each wAlbA and wAlbB strain in Greek populations. The same MLST profiles were also reported in populations from China, Russia, and Argentina, suggesting low levels of global diversity in wAlbA and wAlbB strains. Furthermore, our results indicated a significant association between temperature and the prevalence of single infections (P = 6.498E-07), with higher temperatures correlating with an increased likelihood of single infections. Although male-bias showed a tendency towards single infections, the effect was marginally non-significant (P = 0.053). These results were confirmed using a bootstrap-with-replacement analysis approach. Overall, our findings offer novel insights into the distribution and species diversity of Wolbachia strains in Greek Ae. albopictus populations, emphasizing the importance of understanding the short-term plastic and adaptive responses of these organisms to environmental stressors and rapid climate change.

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Section: Introductionmentioning

confidence: 99%

Molecular Detection, Characterization, and Temperature-Dependence of Wolbachia Infections in Field Populations of <em>Aedes albopictus</em> (Diptera: Culicidae) Mosquitoes in Greece

Misailidis,

Kotsiou,

Moulistanos

et al. 2023

Preprint

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Diversity of biting midges, mosquitoes and sand flies at four dog shelters in rural and peri-urban areas of Central Morocco

Zahri,

Ahlamine,

Abou-Elaaz

et al. 2024

Parasite

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Blood-feeding arthropods are involved in the transmission of several pathogens that have a major impact on public health. Entomological investigations highlighted the composition, abundance, and diversity of flying hematophagous arthropods at four dog shelters located in central Morocco during an eight-month study, with the aim of discussing their vectorial roles and assessing the risk of these shelters as foci for zoonotic diseases. Monitoring of the arthropod fauna for 64 catch nights resulted in the collection of 2,321 biting midges (Ceratopogonidae), 570 mosquitoes (Culicidae), and 475 sand flies (Psychodidae). Fourteen Culicoides species were recorded and dominant species were Culicoides imicola (55.96%), C. paolae (16.07%), C. circumscriptus (10.29%), and C. newsteadi (5.77%). Three mosquito species were collected, including Culex pipiens s.l. (96.84%), Culiseta longiareolata (2.80%), and Cx. perexiguus (0.36%). Ten sand fly species were collected, including seven Phlebotomus species (62.70%) and three Sergentomyia species (37.30%); Sergentomyia minuta was the most dominant species (34.31%), followed by Phlebotomus sergenti (32.42%), typical Ph. perniciosus (8.63%), Ph. alexandri (6.94%), and Ph. riouxi (6.52%). The coexistence of several vectors in these study areas indicates the potential circulation of a wide range of pathogens, including zoonotic ones, thus requiring the implementation of surveillance and control programs to prevent the emergence and spread of disease outbreaks.

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Orthoflavivirus surveillance in the Netherlands: Insights from a serosurvey in horses & dogs and a questionnaire among horse owners

Streng,

Hakze‐van der Honing,

Graham

et al. 2024

Zoonoses and Public Health

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AimsZoonotic arboviruses (arthropod‐borne) of the Orthoflavivirus genus, such as West Nile virus (WNV), Usutu virus (USUV) and Tick‐borne encephalitis virus (TBEV), are emerging in Northwestern Europe and pose a threat to both human and animal health. In the Netherlands, passive symptomatic surveillance (notification of clinical cases) in horses is one of the main pillars for the early detection of WNV. For such passive surveillance to work properly, horse owners and veterinarians need to recognize symptoms and report suspected cases to the authorities. Currently, little is known about the seroprevalence of orthoflaviviruses in domestic animals in the Netherlands. Therefore, this study aims at identifying the seroprevalence of WNV and USUV in horses and dogs in the Netherlands. Additionally, this study seeks to evaluate the knowledge and perceptions of Dutch horse owners towards mosquito‐borne viruses.Methods and ResultsA cross‐sectional serosurvey in horses and dogs was conducted between May 2021 and May 2022. Serum samples were screened using an ELISA and doubtful and positive samples were confirmed by Virus Neutralization Tests for WNV, USUV and TBEV. A validated questionnaire, the MosquitoWise survey, was used to assess the knowledge and perceptions of Dutch horse owners towards mosquito‐borne viruses between July and October 2022. The serosurvey revealed a low seroprevalence for WNV in horses and no WNV‐positive dogs were found. Similarly, a low USUV seroprevalence was found in dogs. The MosquitoWise survey revealed a high knowledge level for horse owners and high awareness of WNV vaccination but a more limited intent to vaccinate.ConclusionsThe low seroprevalences of WNV and USUV indicate many dogs and horses remain susceptible, offering opportunities for trend analysis and surveillance. However, despite multiple recent detections of WNV, USUV, and TBEV in humans, the role of dogs and horses in early detection of human cases is debatable. High awareness among horse owners and the absence of detected equine WNV cases highlight this uncertainty. Continued surveillance is crucial for detecting increased virus circulation and protecting both animal and human health.

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Evidence of Antibodies against the West Nile Virus and the Usutu Virus in Dogs and Horses from the Southeast of France

Cited by 4 publications

References 39 publications

Molecular Detection, Characterization, and Temperature-Dependence of Wolbachia Infections in Field Populations of <em>Aedes albopictus</em> (Diptera: Culicidae) Mosquitoes in Greece

Molecular Detection, Characterization, and Temperature-Dependence of Wolbachia Infections in Field Populations of <em>Aedes albopictus</em> (Diptera: Culicidae) Mosquitoes in Greece

Diversity of biting midges, mosquitoes and sand flies at four dog shelters in rural and peri-urban areas of Central Morocco

Orthoflavivirus surveillance in the Netherlands: Insights from a serosurvey in horses & dogs and a questionnaire among horse owners

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