Evidence of Campylobacter jejuni reduction in broilers with early synbiotic administration

Baffoni, Loredana; Gaggìa, Francesca; Garofolo, Giuliano; Serafino, Gabriella Di; Buglione, Enrico; Giannatale, Elisabetta Di; Gioia, Diana Di

doi:10.1016/j.ijfoodmicro.2017.04.001

Cited by 38 publications

(24 citation statements)

References 34 publications

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“…In light of the increase in antibiotic resistant C. jejuni ( Kassem et al, 2016a ), there is a need to proactively devise alternative approaches to control the proliferation of this pathogen and cognate infections. Several studies have recently investigated the effect of probiotic bacteria on C. jejuni infections in cell lines ( in vitro ) and in animal models such as chickens, a primary host and source of this bacterium ( Baffoni et al, 2017 ; Ekmekciu et al, 2017 ; Johnson et al, 2017 ). Lactobacillus , Bacillus , and Enterococcus have been among the most commonly researched probiotic bacteria against C. jejuni ( Johnson et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of the Impact of the Probiotic E. coli Nissle 1917 on Campylobacter jejuni’s Invasion and Intracellular Survival in Human Colonic Cells

et al. 2017

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Campylobacter jejuni is a leading cause of bacterial food poisoning in humans. Due to the rise in antibiotic-resistant Campylobacter, there exists a need to develop antibiotic-independent interventions to control infections in humans. Here, we evaluated the impact of Escherichia coli Nissle 1917 (EcN), a probiotic strain, on C. jejuni’s invasion and intracellular survival in polarized human colonic cells (HT-29). To further understand how EcN mediates its impact, the expression of 84 genes associated with tight junctions and cell adhesion was profiled in HT-29 cells after treatment with EcN and challenge with C. jejuni. The pre-treatment of polarized HT-29 cells with EcN for 4 h showed a significant effect on C. jejuni’s invasion (∼2 log reduction) of the colonic cells. Furthermore, no intracellular C. jejuni were recovered from EcN pre-treated HT-29 cells at 24 h post-infection. Other probiotic strains tested had no significant impact on C. jejuni invasion and intracellular survival. C. jejuni decreased the expression of genes associated with epithelial cells permeability and barrier function in untreated HT-29 cells. However, EcN positively affected the expression of genes that are involved in enhanced intestinal barrier function, decreased cell permeability, and increased tight junction integrity. The results suggest that EcN impedes C. jejuni invasion and subsequent intracellular survival by affecting HT-29 cells barrier function and tight junction integrity. We conclude that EcN might be a viable alternative for controlling C. jejuni infections.

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Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of the Impact of the Probiotic E. coli Nissle 1917 on Campylobacter jejuni’s Invasion and Intracellular Survival in Human Colonic Cells

et al. 2017

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“…In vivo studies in poultry using synbiotic combinations of Bifidobacterium longum PCB 133 and galactooligosaccharides in animal feed have been shown to reduce C. jejuni infection, demonstrating the potential to reduce transmission along the food chain, which is of fundamental importance for the safety of poultry meat consumers [53]. Microencapsulated Bifidobacterium longum PCB133 and xylooligosaccharides (XOS), when combined, have also been shown to have a synbiotic effect on improving the safety of poultry meat by protecting against C. jejuni infection (p-value: < 0.01) at the beginning of life, while the microbiota is still developing [54]. Additionally, in vivo studies in chicks have demonstrated the ability of three chicken commensal isolates to induce a 1-2 log reduction in Campylobacter numbers.…”

Section: Prebiotic Effects Of Gmomentioning

confidence: 99%

Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Quinn

Slattery

Walsh

et al. 2020

Foods

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Bifidobacteria are known to inhibit, compete with and displace the adhesion of pathogens to human intestinal cells. Previously, we demonstrated that goat milk oligosaccharides (GMO) increased the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to intestinal cells in vitro. In this study, we aimed to exploit this effect as a mechanism for inhibiting pathogen association with intestinal cells. We examined the synergistic effect of GMO-treated B. infantis on preventing the attachment of a highly invasive strain of Campylobacter jejuni to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 42% compared to the control (non-GMO treated B. infantis). Increasing the incubation time of the GMO with the Bifidobacterium strain resulted in the strain metabolizing the GMO, correlating with a subsequent 104% increase in growth over a 24 h period when compared to the control. Metabolite analysis in the 24 h period also revealed increased production of acetate, lactate, formate and ethanol by GMO-treated B. infantis. Statistically significant changes in the GMO profile were also demonstrated over the 24 h period, indicating that the strain was digesting certain structures within the pool such as lactose, lacto-N-neotetraose, lacto-N-neohexaose 3 -sialyllactose, 6 -sialyllactose, sialyllacto-N-neotetraose c and disialyllactose. It may be that early exposure to GMO modulates the adhesion of B. infantis while carbohydrate utilisation becomes more important after the bacteria have transiently colonised the host cells in adequate numbers. This study builds a strong case for the use of synbiotics that incorporate oligosaccharides sourced from goat s milk and probiotic bifidobacteria in functional foods, particularly considering the growing popularity of formulas based on goat milk. Foods 2020, 9, 348 2 of 15Listeria monocytogenes, and Clostridium difficile [4][5][6]. Microbe-associated molecular patterns (MAMPs) are recognized by the host s intestinal pattern recognition receptors (PRRs), and these interactions play key roles in the association of pathogens with the intestinal epithelia. Probiotics also express molecular patterns which can recognize the same trans-membrane receptors as the pathogens, thus blocking the sites for pathogenic contact by competitive exclusion and, in some cases, displacing already-attached pathogens [7]. However, the health benefits associated with bifidobacteria are reliant on such strains colonising the host in sufficient numbers [8]. The important step in microbial colonisation of the intestinal epithelium is the attachment of bacterial surface lectins to intestinal sugar structures. Recent studies have suggested that milk oligosaccharides may enhance the specific ability of bifidobacteria to attach to the GI epithelium [9][10][11]. Indeed, our group investigated the ability of goat milk oligosaccharides (GMO) to increase the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to HT-29 cells. Exposure of the strain ...

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“…For example, there was a significant reduction in C. jejuni in faeces of naturally infected chickens after supplementation for 15 days in two Italian studies (Baffoni et al., ; Santini et al., ). In another study, chickens were fed a synbiotic product, Bifidobacterium longum mixed with a xylo‐oligosaccharide (Baffoni et al., ). One‐day‐old chicks were infected with C. jejuni strain, and a synbiotic mixture was fed twice, on Day 1 and Day 14, of the chicken's life.…”

Section: Prevention Of Colonization and Contaminationmentioning

confidence: 99%

Knowledge gaps in control ofCampylobacterfor prevention of campylobacteriosis

Hansson

Sandberg

Habib

et al. 2018

Transbound Emerg Dis

145

112

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Campylobacteriosis is an important, worldwide public health problem with numerous socio-economic impacts. Since 2015, approximately 230,000 cases have been reported annually in Europe. In the United States, Australia and New Zealand, campylobacteriosis is the most commonly reported disease. Poultry and poultry products are considered important sources of human infections. Poultry meat can become contaminated with Campylobacter during slaughter if live chickens are intestinal carriers. Campylobacter spp. can be transferred from animals to humans through consumption and handling of contaminated food products, with fresh chicken meat being the most commonly implicated food type. Regarding food-borne disease, the most important Campylobacter species are Campylobacter jejuni and Campylobacter coli. In humans, clinical signs of campylobacteriosis include diarrhoea, abdominal pain, fever, headache, nausea and vomiting. Most cases of campylobacteriosis are sporadic and self-limiting, but there are post-infection complications, for example, Guillain-Barrés syndrome. This review summarizes an analysis undertaken by the DISCONTOOLS group of experts on campylobacteriosis. Gaps were identified in: (i) knowledge of true number of infected humans; (ii) mechanisms of pathogenicity to induce infection in humans; (iii) training to prevent transfer of Campylobacter from raw to ready-to-eat food; (iv) development of effective vaccines; (v) understanding transmission routes to broiler flocks; (vi) knowledge of bacteriocins, bacteriophages and antimicrobial peptides as preventive therapies; (vii) ration formulation as an effective preventive measure at a farm level; (viii) development of kits for rapid detection and quantification of Campylobacter in animals and food products; and (ix) development of more effective antimicrobials for treatment of humans infected with Campylobacter. Some of these gaps are relevant worldwide, whereas others are more related to problems encountered with Campylobacter in industrialized countries.

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Evidence of Campylobacter jejuni reduction in broilers with early synbiotic administration

Cited by 38 publications

References 34 publications

In Vitro Evaluation of the Impact of the Probiotic E. coli Nissle 1917 on Campylobacter jejuni’s Invasion and Intracellular Survival in Human Colonic Cells

In Vitro Evaluation of the Impact of the Probiotic E. coli Nissle 1917 on Campylobacter jejuni’s Invasion and Intracellular Survival in Human Colonic Cells

Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Knowledge gaps in control ofCampylobacterfor prevention of campylobacteriosis

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