Evidence of chelonid herpesvirus 5 infection in green turtle (Chelonia mydas) indicated a possible tumorigenesis activation by transcriptome analysis

Abstract

Chelonia mydas (green turtles) are being threatened worldwide by fibropapillomatosis (FP), which has seriously affected their survival. The presence of FP on the body surface and visceral organs of green turtles found dead was confirmed, causing obstruction of the gastrointestinal tract, changes in foraging behavior, and reduction of visceral functions. The etiology of FP has not yet been elucidated, and previous research generally considers that the occurrence of FP is related to the chelonid alphaherpesvirus 5 (ChHV5), associated with low animal immunity, and also with marine environmental factors, such as poor water quality and eutrophication. However, there is no evaluation on the induction of FP pathogenesis associated with the green turtle. In this study, we evaluated blood samples from green turtles with and without FP using de novo transcriptome assembly. Results indicated that 3,090 differentially expressed genes (DEGs) (p < 0.05) were identified, including 1,357 upregulated genes and 1,733 downregulated genes in turtles with or without FP. We observed that DEGs, which are significantly upregulated, are found in cancer development, namely, MAPK1IP1L and APAF1. Furthermore, the infected green turtle indicated that the greater number of DEGs was contributed by the NOD-like receptor signaling pathway, which can be activated through an endocytosis of the viral particle by the immune system cells, and the Wnt signaling pathway, which is believed to have played a role in FP tumorigenesis. We validated the more upregulated/downregulated DEGs in cancer development and immunization, and DEGs such as LEF1, BTRC, and FOSL1 participating in the NOD-like receptor signaling pathway, as well as ERBIN, TRAF6, and NFKB1 in the Wnt signaling pathway, using real-time quantitative polymerase chain reaction (RT-qPCR). Altogether, this study provided some genes as potential markers during FP infection and a further evidence of FP in endangered green turtles in Taiwan.

Assessing the Relationship between Sea Turtle Strandings and Anthropogenic Impacts in Taiwan

“…Reports on FP in green turtles in Asia are still very limited. However, the disease has recently been described in endangered green turtles in Taiwan [6,44,51].…”

“…Reports of FP in sea turtles in Asia are still very limited. However, cases of FP have been discovered in Taiwanese waters in recent years [6,44,51]. Another issue related to environmental contaminants in marine ecology research is that the green turtles in Taiwan may have encountered coastal pollution containing antimicrobial agents or heavy metals when they migrated to nearshore feeding areas after recruitment.…”

The tale of the sea turtle flipper tagging technique, a global environmental impact assessment

“…Sea turtle fibropapillomatosis (FP) is an emerging infectious neoplastic aquatic disease affecting sea turtle populations since the beginning of the 20th century, primarily endangering green turtles Chelonia mydas at the panzootic scale since the 1990s, with new reports of geographically localized epizooties (e.g., Burge et al, 2014;Duffy et al, 2018;James et al, 2021;Loureiro & Matos, 2009;Manes, 2023;Origlia et al, 2023). Recently characterized to show similar genetic pathways with human basal cell carcinoma (Duffy et al, 2018;Li et al, 2023), clinical cases of sea turtle FP are strongly correlated to the detection of oncogenic chelonid alphaherpesvirus 5 ChHV5 (e.g., Duffy et al, 2018;Hargrove et al, 2016;Mashkour et al, 2020;Stacy et al, 2017). The disease impacts the health and ecological roles of keystone marine species.…”