Evidence of hypericin photoinactivation of E. faecalis: From planktonic culture to mammalian cells selectivity up to biofilm disruption

Amaral, Larissa Souza; Ribeiro, Anderson Orzari; Perussi, Janice Rodrigues

doi:10.1016/j.pdpdt.2020.101759

Cited by 3 publications

(4 citation statements)

References 61 publications

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“…The hypericin-mediated PDI reduced the biofilms of Propionibacterium acnes by 14.1% and 27.9%, respectively, at a dose of 100 and 166 J/cm 2 LED irradiation (660 nm), respectively (Barroso et al, 2021). Furthermore, Amaral et al (2020) revealed that this PDI had a cell selectivity, because the hypericin could be rapidly absorbed by Enterococcus faecalis. And the PDI treatment eliminated 60% of the cells in the biofilms of E. faecalis when subjected to 590 ± 10 nm LED irradiation.…”

Section: Gram-positive Bacterial Biofilmsmentioning

confidence: 91%

“…Usually, most of bacteria exist in the form of biofilms, which account for ∼80% of foodborne diseases (Chen et al., 2019a, b). Many studies have widely demonstrated that the PDI is a feasible method to eradicate biofilms (Amaral et al., 2020; Barros et al., 2020; Barroso et al., 2021). The PDI treatment not only fatally inactivates the planktonic cells to prevent the biofilm formation, but also destroys the EPS of mature biofilms, as well as the architectural structures (thickness, volume, porosity, homogeneity, roughness, etc.)…”

Section: Eradication Potency Of Pdi Against Biofilmsmentioning

confidence: 99%

“…Furthermore, Amaral et al. (2020) revealed that this PDI had a cell selectivity, because the hypericin could be rapidly absorbed by Enterococcus faecalis . And the PDI treatment eliminated 60% of the cells in the biofilms of E. faecalis when subjected to 590 ± 10 nm LED irradiation.…”

Section: Eradication Potency Of Pdi Against Biofilmsmentioning

confidence: 99%

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New trends in the development of photodynamic inactivation against planktonic microorganisms and their biofilms in food system

Chen,

Zhao,

et al. 2023

Comp Rev Food Sci Food Safe

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The photodynamic inactivation (PDI) is a novel and effective nonthermal inactivation technology. This review provides a comprehensive overview on the bactericidal ability of endogenous photosensitizers (PSs)‐mediated and exogenous PSs‐mediated PDI against planktonic bacteria and their biofilms, as well as fungi. In general, the PDI exhibited a broad‐spectrum ability in inactivating planktonic bacteria and fungi, but its potency was usually weakened in vivo and for eradicating biofilms. On this basis, new strategies have been proposed to strengthen the PDI potency in food system, mainly including the physical and chemical modification of PSs, the combination of PDI with multiple adjuvants, adjusting the working conditions of PDI, improving the targeting ability of PSs, and the emerging aggregation‐induced emission luminogens (AIEgens). Meanwhile, the mechanisms of PDI on eradicating mono‐/mixed‐species biofilms and preserving foods were also summarized. Notably, the PDI‐mediated antimicrobial packaging film was proposed and introduced. This review gives a new insight to develop the potent PDI system to combat microbial contamination and hazard in food industry.

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Section: Gram-positive Bacterial Biofilmsmentioning

confidence: 91%

Section: Eradication Potency Of Pdi Against Biofilmsmentioning

confidence: 99%

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New trends in the development of photodynamic inactivation against planktonic microorganisms and their biofilms in food system

Chen,

Zhao,

et al. 2023

Comp Rev Food Sci Food Safe

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“…For example, a nanoparticle formulation of Hyp (45 nm diameter) was able to completely eradicate infection in rats presenting wounds infected with methicillin-resistant S. aureus [ 349 ]. This result is interesting considering that, in absence of carriers, Hyp showed only moderate anti-biofilm activity [ 350 ], even though results were obtained under different conditions and for different types of bacteria.…”

Section: Applications To Biofilm Eradicationmentioning

confidence: 99%

Photodynamic treatment of pathogens

2022

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The current viral pandemic has highlighted the compelling need for effective and versatile treatments, that can be quickly tuned to tackle new threats, and are robust against mutations. Development of such treatments is made even more urgent in view of the decreasing effectiveness of current antibiotics, that makes microbial infections the next emerging global threat. Photodynamic effect is one such method. It relies on physical processes proceeding from excited states of particular organic molecules, called photosensitizers, generated upon absorption of visible or near infrared light. The excited states of these molecules, tailored to undergo efficient intersystem crossing, interact with molecular oxygen and generate short lived reactive oxygen species (ROS), mostly singlet oxygen. These species are highly cytotoxic through non-specific oxidation reactions and constitute the basis of the treatment. In spite of the apparent simplicity of the principle, the method still has to face important challenges. For instance, the short lifetime of ROS means that the photosensitizer must reach the target within a few tens nanometers, which requires proper molecular engineering at the nanoscale level. Photoactive nanostructures thus engineered should ideally comprise a functionality that turns the system into a theranostic means, for instance, through introduction of fluorophores suitable for nanoscopy. We discuss the principles of the method and the current molecular strategies that have been and still are being explored in antimicrobial and antiviral photodynamic treatment.

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Photodynamic disinfection and its role in controlling infectious diseases

Aroso

Schaberle

Arnaut

et al. 2021

Photochem Photobiol Sci

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Photodynamic therapy is witnessing a revival of its origins as a response to the rise of multi-drug resistant infections and the shortage of new classes of antibiotics. Photodynamic disinfection (PDDI) of microorganisms is making progresses in preclinical models and in clinical cases, and the perception of its role in the clinical armamentarium for the management of infectious diseases is changing. We review the positioning of PDDI from the perspective of its ability to respond to clinical needs. Emphasis is placed on the pipeline of photosensitizers that proved effective to inactivate biofilms, showed efficacy in animal models of infectious diseases or reached clinical trials. Novel opportunities resulting from the COVID-19 pandemic are briefly discussed. The molecular features of promising photosensitizers are emphasized and contrasted with those of photosensitizers used in the treatment of solid tumors. The development of photosensitizers has been accompanied by the fabrication of a variety of affordable and customizable light sources. We critically discuss the combination between photosensitizer and light source properties that may leverage PDDI and expand its applications to wider markets. The success of PDDI in the management of infectious diseases will ultimately depend on the efficacy of photosensitizers, affordability of the light sources, simplicity of the procedures, and availability of fast and efficient treatments. Graphic abstract

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Evidence of hypericin photoinactivation of E. faecalis: From planktonic culture to mammalian cells selectivity up to biofilm disruption

Cited by 3 publications

References 61 publications

New trends in the development of photodynamic inactivation against planktonic microorganisms and their biofilms in food system

New trends in the development of photodynamic inactivation against planktonic microorganisms and their biofilms in food system

Photodynamic treatment of pathogens

Photodynamic disinfection and its role in controlling infectious diseases

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