Evidence of intracellular and trans‐acting differentiation‐inducing activity in human promyelocytic leukemia HL‐60 cells: Its possible involvement in process of cell differentiation from a commitment step to a phenotype‐expression step

Okazaki, Toshiro; Kato, Yoshiki; Tashima, Masaro; Sawada, Hiroyoshi; Uchino, Haruto

doi:10.1002/jcp.1041340212

Cited by 12 publications

(6 citation statements)

References 38 publications

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“…To date, there are no studies that have yet been reported on the use of cybridization to direct chondrogenic differentiation. Nevertheless, previous studies have shown that cybridization could be used to induce teratocarcinoma cells to express myoblast function [218, 219] as well as direct erythroid [220, 221] and myeloid [222] differentiation.…”

Section: Future Perspectivesmentioning

confidence: 99%

Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro

2004

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A major area in regenerative medicine is the application of stem cells in cartilage tissue engineering and reconstructive surgery. This requires well‐defined and efficient protocols for directing the differentiation of stem cells into the chondrogenic lineage, followed by their selective purification and proliferation in vitro. The development of such protocols would reduce the likelihood of spontaneous differentiation of stem cells into divergent lineages upon transplantation, as well as reduce the risk of teratoma formation in the case of embryonic stem cells. Additionally, such protocols could provide useful in vitro models for studying chondrogenesis and cartilaginous tissue biology. The development of pharmacokinetic and cytotoxicity/genotoxicity screening tests for cartilage‐related biomaterials and drugs could also utilize protocols developed for the chondrogenic differentiation of stem cells. Hence, this review critically examines the various strategies that could be used to direct the differentiation of stem cells into the chondrogenic lineage in vitro.

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Section: Future Perspectivesmentioning

confidence: 99%

Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro

2004

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“…Together with the findings that uremic serum confers l,25-(OH)2D3 resistance upon the HL-60 cells in the induction of cell differentiation and of 24-hydroxylation activity partly by decreasing the l,25-(OH)2D3 receptor content, these data raise the possibility that a possible decrease in the intracellular cAM P level might contribute to the l,25-(OH)2D3 resistance observed in the uremic serumtreated cells. An incubation time of 2 days was selected to measure intracellular cAMP levels because, around 2 days of exposure to l,25-(OH)2D3, the cells so treated progress from the commitment step to the phenotype-expression step [28]. As shown in figure 4, intracellular cAMP levels in the uremic serum-treated cells (n = 4) exhibited 9.85 ± 1.16 pmol/107 cells as compared with 13.7 ±3.20 pmol/107 cells of the normal serum-treated cells (n = 3, p<0.05).…”

Section: Effect O F Uremic Serum On Intracellular Camp Content O F Hlmentioning

confidence: 99%

Effect of Uremic Serum on 1,25-Dihydroxyvitamin D<sub>3</sub>-lnduced Differentiation of Human Promyelocytic Leukemia Cell, HL-60

Inaba

Okuno²,

Koyama³

et al. 1993

Nephron

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The mechanism by which resistance to 1,25-(OH)₂D₃ occurs in patients with chronic renal failure was studied. 1,25-(OH)₂D₃ causes the induction of differentiation and of 1,25-(OH)₂D₃-24-hydroxylase activity in the mitochondria of the human promyelocytic leukemia cell line, HL-60, via a steroid hormone-receptor mechanism. Treatment of these cells with 10 ⁸M 1,25-(OH)₂D₃ for 5 days in a medium containing 10% uremic serum from 4 patients with chronic renal failure resulted in maturation of the cells amounting to 30.3 ± 18.7 (mean ± SD) and 32.5 ± 11.2% maturation by the nitroblue tetrazolium reduction assay and the nonspecific esterase assay, respectively. These values were significantly lower than those obtained with 10% normal serum from 3 normal controls (66.6 ± 12.8 and 58.3 ± 10.9%, p < 0.02). The occurrence of resistance to 1,25-(OH)₂D₃ in uremic serum-treated cells was also confirmed when the effect of 1,25-(OH)₂D₃was assessed by the induction of the cell’s ability to hydroxylate the C-24 position of 1,25-(OH)₂[³H]D₃. Treatment of HL-60 cells with a mixture of 5% uremic plus 5% normal serum impaired 1,25-(OH)₂D₃-induced cell differentiation to the levels as those in 10% uremic serum, strongly suggesting the occurrence of a substance(s) having 1,25-(OH)₂D₃-inhibitory activity in the uremic serum. A significant reduction in 1,25-(OH)₂D₃ receptor levels was observed in uremic serum-treated cells. We have recently reported that the addition of dibutyryl cAMP significantly enhances the effect of l,25-(OH)₂D₃ on HL-60 cells by increasing 1,25-(OH)₂D₃ receptor levels and that a significant positive correlation was observed between intracellular cAMP levels and 1,25-(OH)₂D₃-induced HL-60 cell maturation. Together with the data that treatment of the cells with uremic serum resulted in a significant reduction in intracellular cAMP levels, the significance of cAMP in the occurrence of 1,25-(OH)₂D₃-resistance in uremic serum-treated cells is indicated.

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“…To date, there are no studies that have reported the use of cybridization to direct stem cell differentiation. Previous studies have shown that cybridization could be used to induce teratocarcinoma cells to express myoblast function (Iwakura et al 1985;Tosu et al 1988), as well as direct erythroid (Watanabe et al 1985(Watanabe et al , 1988 and myeloid (Okazaki et al 1988) differentiation.…”

Section: Cybridization To Direct Stem Cell Differentiationmentioning

confidence: 99%

An overview and synopsis of techniques for directing stem cell differentiation in vitro

Heng

Cao

Haider

et al. 2004

Cell and Tissue Research

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The majority of studies on stem cell differentiation have so far been based in vivo, on live animal models. The usefulness of such models is limited, since it is much more technically challenging to conduct molecular studies and genetic manipulation on live animal models compared to in vitro cell culture. Hence, it is imperative that efficient protocols for directing stem cell differentiation into well-defined lineages in vitro are developed. The development of such protocols would also be useful for clinical therapy, since it is likely that the transplantation of differentiated stem cells would result in higher engraftment efficiency and enhanced clinical efficacy, compared to the transplantation of undifferentiated stem cells. The in vitro differentiation of stem cells, prior to transplantation in vivo, would also avoid spontaneous differentiation into undesired lineages at the transplantation site, as well as reduce the risk of teratoma formation, in the case of embryonic stem cells. Hence, this review critically examines the various strategies that could be employed to direct and control stem cell differentiation in vitro.

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Evidence of intracellular and trans‐acting differentiation‐inducing activity in human promyelocytic leukemia HL‐60 cells: Its possible involvement in process of cell differentiation from a commitment step to a phenotype‐expression step

Cited by 12 publications

References 38 publications

Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro

Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro

Effect of Uremic Serum on 1,25-Dihydroxyvitamin D<sub>3</sub>-lnduced Differentiation of Human Promyelocytic Leukemia Cell, HL-60

An overview and synopsis of techniques for directing stem cell differentiation in vitro

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