Evidence of lateral transmission of aggressive features between different types of breast cancer cells

Abstract

Breast cancer (BrC) is a major public health problem worldwide. The intra-tumoral heterogeneity and tumor cell plasticity importantly contribute to disease progression and treatment failure. However, the dynamic interactions between different tumor clones, as well as their contribution to tumor aggressiveness are still poorly understood. In this study, we provide evidence of a lateral transmission of aggressive features between aggressive and non-aggressive tumor cells, consisting of gain of expression of cancer stem cell markers, increased expression of CXCL12 receptors CXCR4 and CXCR7 and increased invasiveness in response to CXCL12, which correlated with high levels of secretion of pro-inflammatory mediators G-CSF, GM-CSF, MCP-1, IL-8 and metalloproteinases 1 and 2 by the aggressive cells. Noteworthy, we found no evidence of a TGF-β participation in the inducible-invasive phenotype. Altogether, our results provide evidence of communication between tumor cells with different potentials for aggressiveness, which could influence intra-tumoral population dynamics promoting the emergence of clones with novel functions. Understanding these interactions will provide better targets for diagnosis, prognosis and therapeutic strategies.

MicroRNA-1254 exerts�oncogenic effects by directly targeting RASSF9 in human breast cancer

“…Although breast cancer diagnosis and therapy have both improved, satisfactory therapeutic effects have not yet been achieved due to disease complexity. Tumorigenesis is a multifactorial and multistep process involving a range of genetic alterations, including the activation of oncogenes, the inactivation of anti-oncogenes and the abnormal expression of cancer-related genes (4)(5)(6). Previous studies have unraveled some of the pathological mechanisms involved in breast tumorigenesis (7,8); however, these have yet to be fully elucidated.…”

Human breast tumour cells viability effect of African Dioscorea Rotundata Tuber extracts in MCF-7 and MDA-MB231 cell lines

“…5A and B); but either DiosB or DiosR exerted a time dependent action on MDA-MB231 cells being more effective at 48 h than at 24 h (Fig 6). In spite of this, both extracts were more effective on MCF-7 than on MDA-MB231 cells showing a higher maximal effect on MCF-7 cells than on MDA-MB231 either after 24 h or 48 h of treatment (p<0.0001 and p<0.05 respectively) probably due to the high aggressiveness and invasiveness documented by many authors for triple negative MDA-MB231 tumor cells ( Espinoza-Sánchez et al, 2017, Jeonghun et al, 2019.…”

Human Breast Tumour Cells Viability Effect of African Dioscorea Rotundata Tuber Extracts in McF-7 and Mda-Mb231 Cell Lines

“…after 24 h or 48 h of treatment (p<0.0001 and p<0.05 respectively) probably due to the high aggressiveness and invasiveness documented by many authors for triple negative MDA-MB231 tumor cells ( 20,21).…”