Evidence of Limited Polyandry in a Natural Population of Aedes aegypti

Richardson, Joshua B.; Jameson, Samuel; Gloria‐Soria, Andrea; Wesson, Dawn M.; Powell, Jeffrey R.

doi:10.4269/ajtmh.14-0718

Cited by 36 publications

(33 citation statements)

References 32 publications

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“…We defined paternity enforcement as the ability of a male to father all offspring produced by a female despite her subsequent exposure to other potential mates. Previous work reported levels of remating in the field [20] and in semi-field conditions as high as 14% [21]. It is important to note that these earlier experiments used different methods to establish paternity, either microsatellite analysis of offspring [20] or detection of labeled seminal fluid derived from two different males [21].…”

Section: Resultsmentioning

confidence: 99%

“…Previous work reported levels of remating in the field [20] and in semi-field conditions as high as 14% [21]. It is important to note that these earlier experiments used different methods to establish paternity, either microsatellite analysis of offspring [20] or detection of labeled seminal fluid derived from two different males [21]. Our laboratory work directly scored the genotype of each live offspring using the ECFP paternity marker strain.…”

Section: Resultsmentioning

confidence: 99%

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A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito

et al. 2017

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SUMMARY Female Aedes aegypti mosquitoes typically mate only once with one male in their lifetime, a behavior known as “monandry” [1]. This single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ~4–6-week lifespan in the laboratory [2]. Successful mating induces lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male [3–5]. Ae. aegypti mate in flight near human hosts [6], and females become refractory to remating within seconds [1, 3, 4], suggesting the existence of a rapid mechanism to prevent female remating. In this study we implicate HP-I, an Aedes- and male-specific peptide transferred to females [7], and its cognate receptor in the female, NPYLR1 [8], in rapid enforcement of paternity. HP-I mutant males were ineffective in enforcing paternity when a second male was given access to the female within 1 hour. NPYLR1 mutant females produced mixed paternity offspring at high frequency, indicating acceptance of multiple mates. Synthetic HP-I injected into wild-type but not NPYLR1 mutant virgins reduced successful matings. Asian tiger mosquito (Ae. albopictus) HP-I peptides potently activated Ae. aegypti NPYLR1. Invasive Ae. albopictus males are known to copulate with and effectively sterilize Ae. aegypti females by causing them to reject future mates [9]. Cross-species transfer of sperm and active seminal fluid proteins including HP-I may contribute to this phenomenon. This signaling system promotes rapid paternity enforcement within Ae. aegypti, but may promote local extinction in areas where they compete with Ae. albopictus.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito

et al. 2017

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“…While mosquito sperm do not form conjugated groups like other taxa (Hayashi, 1998;Higginson et al, 2012), it is possible that mosquito sperm reduce drag by swimming in parallel, allowing for their faster storage in the spermathecae. Females of some mosquitoes may occasionally mate more than once (Boyer et al, 2012;Richardson et al, 2015), especially soon after their first mating (Degner and Harrington, 2016). Therefore, males whose sperm fill the spermathecae quickly may reduce their risk of sharing paternity with a second male.…”

Section: Mechanisms Of Spermathecal Fillingmentioning

confidence: 99%

A mosquito sperm's journey from male ejaculate to egg: Mechanisms, molecules, and methods for exploration

Degner

Harrington

2016

Molecular Reproduction Devel

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SUMMARYThe fate of mosquito sperm in the female reproductive tract has been addressed sporadically and incompletely, resulting in significant gaps in our understanding of sperm‐female interactions that ultimately lead to fertilization. As with other Diptera, mosquito sperm have a complex journey to their ultimate destination, the egg. After copulation, sperm spend a short time at the site of insemination where they are hyperactivated and quickly congregate near the entrance of the spermathecal ducts. Within minutes, they travel up the narrow ducts to the spermathecae, likely through the combined efforts of female transport and sperm locomotion. The female nourishes sperm and maintains them in these permanent storage organs for her entire life. When she is ready, the female coordinates the release of sperm with ovulation, and the descending egg is fertilized. Although this process has been well studied via microscopy, many questions remain regarding the molecular processes that coordinate sperm motility, movement through the reproductive tract, maintenance, and usage. In this review, we describe the current understanding of a mosquito sperm's journey to the egg, highlighting gaps in our knowledge of mosquito reproductive biology. Where insufficient information is available in mosquitoes, we describe analogous processes in other organisms, such as Drosophila melanogaster, as a basis for comparison, and we suggest future areas of research that will illuminate how sperm successfully traverse the female reproductive tract. Such studies may yield molecular targets that could be manipulated to control populations of vector species. Mol. Reprod. Dev. 83: 897–911, 2016. © 2016 The Authors. Molecular Reproduction and Development Published by Wiley Periodicals, Inc.

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“…We considered half-siblings to be paternal because polyandry is much 290 rarer than polygyny in wild Ae. aegypti (Richardson et al, 2015). Therefore, we assumed full-siblings 291 to come from the same matrilineage, and half-siblings from different matrilineages.…”

Section: Individuals 210mentioning

confidence: 99%

Fine-scale landscape genomics helps explain the slow spread ofWolbachiathrough theAedes aegyptipopulation in Cairns, Australia

Schmidt

Filipović

Hoffmann

et al. 2017

Preprint

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The endosymbiotic bacterium Wolbachia suppresses the capacity for arboviral transmission in the mosquito Aedes aegypti, and can spread through wild mosquito populations following local introductions. Recent introductions in Cairns, Australia have demonstrated slower than expected spread, that could be due to: i) barriers to Ae. aegypti dispersal; ii) leptokurtically distributed dispersal distances; and iii) intergenerational loss of Wolbachia. We investigated these three potential causes using genome-wide single-nucleotide polymorphisms (SNPs) and an assay for the Wolbachia infection wMel in 161 Ae. aegypti collected from Cairns in 2015. We observed a significant barrier effect of Cairns highways on Ae. aegypti dispersal using distance-based redundancy analysis and patch-based simulation analysis. We detected putative full-siblings in ovitraps 1312m apart, suggesting long-distance female movement likely mediated by human transport. Finally, we found a pair of full-siblings of different infection status, suggesting loss of Wolbachia in the field. While the long-distance movement and Wolbachia loss currently represent single observations, these findings together with the identified dispersal barriers can contribute to the slow spread of Wolbachia through the Ae. aegypti population in Cairns. Our landscape genomics approach can be extended to other host/symbiont systems that are being considered for biocontrol.

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Evidence of Limited Polyandry in a Natural Population of Aedes aegypti

Cited by 36 publications

References 32 publications

A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito

A Peptide Signaling System that Rapidly Enforces Paternity in the Aedes aegypti Mosquito

A mosquito sperm's journey from male ejaculate to egg: Mechanisms, molecules, and methods for exploration

Fine-scale landscape genomics helps explain the slow spread ofWolbachiathrough theAedes aegyptipopulation in Cairns, Australia

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