Evidence of ljungan virus specific antibodies in humans and rodents, Finland

Jääskeläinen, Anne J.; Kolehmainen, Pekka; Voutilainen, Liina; Hauffe, Heidi C.; Kallio‐Kokko, Hannimari; Lappalainen, Maija; Tolf, Conny; Lindberg, Anders; Henttonen, Heikki; Vaheri, Antti; Tauriainen, Sisko; Vapalahti, Olli

doi:10.1002/jmv.23681

Cited by 22 publications

(39 citation statements)

References 35 publications

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“…In total, 47.8% of patient samples were LV seropositive, higher than reported previously in Finland (38% [14]; 36% [15]. However, [15] reported that the seroprevalence of LV in the age group of 30-39 years was 41%; similarly, in this study most of the LV-seropositive acute NE patients were in the 31-40 years age group.…”

Section: Discussioncontrasting

confidence: 67%

“…Serum samples from acute NE patients were analysed by IFA tests for the presence of LCMV, LV and OPV reactive IgG antibodies based on previously described and validated methods [33,17,14], using Vero E6 cells infected with LCMV (strain Armstrong), and Vero cells infected with or LV (strain LV 145 SLG; Johansson et al, 2003 or CPXV (strain CPXV/FIN/T2000, human isolate; [33]. Sera were diluted 1:20 in phosphate-buffered saline (PBS) for initial screening, added to IFA-slides, and incubated in a moist chamber at 37 • C for 30 min.…”

Section: Immunofluorescence Assay (Ifa) and Pcrmentioning

confidence: 99%

“…Samples were recorded as positive or negative in IFA based on the pattern and amount of fluorescence in comparison to the positive and negative controls [14]. All positive samples were positive for titer 20 or more.…”

Section: Immunofluorescence Assay (Ifa) and Pcrmentioning

confidence: 99%

“…The relevant rodentborne viruses include Puumala hantavirus (PUUV), orthopoxvirus (OPV), lymphocytic choriomeningitis virus (LCMV), and Ljungan virus (LV) [1,17,14,41,12]. While many studies have investigated the clinical outcomes of human disease following infection with PUUV (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Lymphocytic choriomeningitis, Ljungan and orthopoxvirus seroconversions in patients hospitalized due to acute Puumala hantavirus infection

Fevola

Forbes

Mäkelä

et al. 2016

Journal of Clinical Virology

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a b s t r a c tBackground: The emergence and re-emergence of zoonotic and vector-borne diseases are increasing in Europe. Prominent rodent-borne zoonotic viruses include Puumala hantavirus (PUUV; the causative agent of nephropathia epidemica, NE), lymphocytic choriomeningitis virus (LCMV), and orthopoxviruses (OPV). In addition, Ljungan virus (LV) is considered a potentially zoonotic virus. Objective: The aim of this study was to compare clinical picture between acute PUUV patients with and without additional rodent-borne viral infections, to investigate if concurrent infections influence disease severity. Study design: We evaluated seroprevalence of and seroconversions to LCMV, LV and OPV in 116 patients hospitalized for NE. Clinical and laboratory variables were closely monitored during hospital care. Results: A total of five LCMV, 15 LV, and one OPV seroconversions occurred. NE patients with LCMV seroconversions were younger, and had lower plasma creatinine concentrations and platelet counts than patients without LCMV seroconversions. No differences occurred in clinical or laboratory findings between patients with and without seroconversions to LV and OPV. We report, for the first time, LCMV seroprevalence in Finland, with 8.5% of NE patients seropositive for this virus. Seroprevalences for LV and OPV were 47.8% and 32.4%, respectively. Conclusion: Cases with LCMV seroconversions were statistically younger, had milder acute kidney injury and more severe thrombocytopenia than patients without LCMV. However, the low number of seroconversion cases precludes firm conclusions. Concurrent LV or OPV infections do not appear to influence clinical picture for NE patients.

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Section: Discussioncontrasting

confidence: 67%

Section: Immunofluorescence Assay (Ifa) and Pcrmentioning

confidence: 99%

Section: Immunofluorescence Assay (Ifa) and Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lymphocytic choriomeningitis, Ljungan and orthopoxvirus seroconversions in patients hospitalized due to acute Puumala hantavirus infection

Fevola

Forbes

Mäkelä

et al. 2016

Journal of Clinical Virology

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“…Heart extracts were tested for antibodies against hantavirus, lymphocytic choriomeningitis virus (LCMV), Ljungan virus (LV) and orthopoxviruses (OPV) using previously described indirect fluorescent antibody tests (IFAT; KallioKokko et al 2006, Jääskeläinen et al 2013). …”

Section: Antibody Detection (I)mentioning

confidence: 99%

Food resources and intestinal parasites as limiting factors for boreal vole populations during winter

Forbes

Stuart

Mappes

et al. 2014

Ecology

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Processes limiting the growth of cyclic vole populations have stimulated considerable research and debate over several decades. In Fennoscandia, the peak density of cyclic vole populations occurs in fall, and is followed by a severe winter decline. Food availability and intestinal parasites have been demonstrated to independently and synergistically limit wildlife populations. The purpose of this study was to directly compare competing food and parasite hypotheses on the limitation of overwintering high‐density vole populations. Moreover, we evaluated the ability of food limitation and nematode infection to interact and thereby intensify population declines. A two‐factor experiment with food supplementation and antihelminthic medication was conducted on replicated, enclosed field vole (Microtus agrestis) populations in central Finland over one full boreal winter. Population abundance, survival, and demographic attributes were monitored through live trapping. Vole feces were concurrently examined for the eggs of Heligmosomidae nemadotes and oocysts of eimerian coccidians. We found that vole density declined in all treatment groups throughout winter. However, food supplementation mitigated this decline through positive effects on reproduction, and voles in food‐supplemented populations were generally in better physiological condition than non‐supplemented voles. Food supplementation and antihelminthic treatment reduced the prevalence of Heligmosomidae nematodes, while neither food nor medication affected the prevalence of eimerians, or infection intensity of either parasite group. Although food supplementation and antihelminthic medication aided in the clearance of Heligmosomidae nematodes, their prevalence did not influence vole population growth, and this parasite group is therefore unlikely to contribute to the cyclic winter decline of boreal vole populations. Instead food resources acting alone were the primary factor limiting vole population growth.

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Evaluation of the association of zoonotic Ljungan virus with perinatal deaths and fetal malformation

Zheng

Fang

Huang

et al. 2015

Birth Defects Research Pt C

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More and more epidemiologic and experimental data support the notion that Ljungan virus (LV), originally isolated from some rodent populations in Sweden, Denmark, and the United States, plays an important role in stillbirth and fetal malformation. Mouse dams infected with LV may result in uterine resorption and perinatal deaths that may cross generations, and their offspring may suffer high rates of malformations including cranial, brain, and limb malformations. In humans, researches founded that LV infection is related to malformation, intrauterine fetal death, and even central nervous system malformation. Although molecularly characterized, little is known about the biophysical nature of LV. Consequently, the role of LV infections in sudden infant death syndrome is still confusing, and the mechanism of how LV infections cause diseases is not clear. More research is clearly necessary to explore the mechanisms of LV infection in human and animal diseases to bring improvement to the clinical outcomes.

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Evidence of ljungan virus specific antibodies in humans and rodents, Finland

Cited by 22 publications

References 35 publications

Lymphocytic choriomeningitis, Ljungan and orthopoxvirus seroconversions in patients hospitalized due to acute Puumala hantavirus infection

Lymphocytic choriomeningitis, Ljungan and orthopoxvirus seroconversions in patients hospitalized due to acute Puumala hantavirus infection

Food resources and intestinal parasites as limiting factors for boreal vole populations during winter

Evaluation of the association of zoonotic Ljungan virus with perinatal deaths and fetal malformation

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