Evidence of Lower Oxidative Stress in the Air Spaces of Patients with Reversible Copd

Serviddio, Gaetano; Carpagnano, Giovanna Elisiana; Rollo, Tiziana; Tamborra, Rosanna; Vendemiale, Gianluigi; Altomare, Emanuele

doi:10.1177/039463200601900318

Cited by 6 publications

(11 citation statements)

References 38 publications

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“…The rate of DTNB oxidation is followed spectrophotometrically as 412 nm of absorbance. GSSG was measured according to Tietze using the modification of Sacchetta (32), as previously reported (31). A specific enzyme immunoassay kit (Cayman Chemical, Ann Harbor, MI) was used to measure TNF-a concentrations in plasma, as previously reported (31).…”

Section: Glutathione and Plasma Assaysmentioning

confidence: 99%

“…GSSG was measured according to Tietze using the modification of Sacchetta (32), as previously reported (31). A specific enzyme immunoassay kit (Cayman Chemical, Ann Harbor, MI) was used to measure TNF-a concentrations in plasma, as previously reported (31). Plasma fluorescent adducts formed between peroxidation-derived aldehydes (MDA and HNE) and plasma protein were monitored by spectrofluorimetry according to Tsuchida, as previously reported (33).…”

Section: Glutathione and Plasma Assaysmentioning

confidence: 99%

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Frailty Syndrome is Associated with Altered Circulating Redox Balance and Increased Markers of Oxidative Stress

Serviddio

Romano

Greco

et al. 2009

Int J Immunopathol Pharmacol

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Frailty Syndrome (FS) is a condition described in aging and characterized by physical vulnerability to stress and lack of physiological reserve. In this study we aim to define whether circulating oxidative stress correlates to frailty in terms of glutathione balance and oxidative protein damage. In 62 elderly outpatients, classified as frail patients according to Fried's criteria, evaluation of reduced Glutathione (GSH), Oxidized Glutathione (GSSG), Tumor Necrosis Factor-α (TNF-α), Malonaldehyde- (MDA) and 4-hydroxy-2,3-nonenal- (HNE) protein plasma adducts were performed. A significant increase in the GSSG was observed in patients with FS when compared to non-frail. No difference was shown in the GSH amount, suggesting a glutathione oxidation more than impairment of the synthesis. TNF-α, MDA- and HNE- adducts, were significantly higher in FS as compared to non-frail patients. A logistic regression model correlating FS with redox balance showed a close relationship between glutathione ratio (OR= 1.8, 95% CI=1.2–2.5) and MDA adducts (OR= 2.8, 95% CI= 1.6–4.7) to frailty. Our findings show an association between oxidative imbalance and Frailty Syndrome. GSSG/GSH ratio and plasma protein adducts strongly predict the frailty conditions and seem to be reliable and easily measurable markers in the context of the multidimensional analysis of elderly patients.

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Section: Glutathione and Plasma Assaysmentioning

confidence: 99%

Section: Glutathione and Plasma Assaysmentioning

confidence: 99%

Frailty Syndrome is Associated with Altered Circulating Redox Balance and Increased Markers of Oxidative Stress

Serviddio

Romano

Greco

et al. 2009

Int J Immunopathol Pharmacol

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“…The role of lung function, and of volume and flow reversibility in predicting disease progression of COPD (e.g. rate of lung function; health status decline, and rate of exacerbations) has been already clarified [Serviddio et al 2006;Calverley et al 2003]: obviously, an improved response to bronchodilators is presumed to impact positively in COPD patients in terms of daily symptoms and quality of life [Omata et al 2007]. …”

Section: Original Researchmentioning

confidence: 99%

Erdosteine enhances airway response to salbutamol in patients with mild-to-moderate COPD

Negro¹,

M²,

Trevisan³

et al. 2008

Ther Adv Respir Dis

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Abstract:Background: Oxidative stress is presumed to impair -adenoceptor function and airway patency. Erdosteine (E), a mucomodulatory compound, has shown important antioxidant properties. Methods:The objective was to assess the effect of antioxidant interventions on short-term airway response to salbutamol in non-reversible mild-to-moderate COPD patients. Thirty COPD patients (GOLD class 1-2), current smoker (!10 pack/year), randomly received E 300 mg, N-acetylcysteine (NAC) 600 mg, or placebo, twice daily for ten days. Reversibility to salbutamol 200 mg was tested in baseline, after four and ten days of each treatment. ROS and 8-isoprostane blood levels were measured on the same days. Between-treatment comparison was performed by ANOVA and t-test or Wilcoxon test, and p50.05 assumed. E enhanced FEV1 reversibility after four and ten days significantly (þ5.1% and þ5.0%; both p50.01 vs. placebo), while NAC only showed a transient effect at day 4 (þ3.0%, p50.05), but not at day 10 (þ1.3%, p ¼ ns).Results: E and NAC caused significant drops in ROS blood levels after four and ten days (p50.001 and p50.0001 vs. placebo). In contrast to NAC, E lowered 8-isoprostane levels substantially for ten days (p ¼ 0.017 and p ¼ 0.0004 vs. placebo, respectively). Only E restored significantly short-term reversibility in COPD patients previously unresponsive to 2 -adrenergics. Conclusions: This effect seems more related to the peculiar protection against lipid peroxidation rather than to the scavenging activity, which proves equal to that of NAC. E provides a sort of indirect bronchodilation through 're-sensitisation' of 2 -adrenoceptors. Once confirmed in further controlled studies, it may be useful in long-term treatment of COPD.

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“…Recently, our group demonstrated that COPD subjects with reversible obstruction present lower oxidative stress in airways (18). These more reversible COPD patients may represent an intermediate group of patients between asthma and non-reversible COPO or may be COPD patients who also have concomitant asthma (19).…”

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confidence: 95%

Inflammation, Oxidative Stress and Systemic Effects in Mild Chronic Obstructive Pulmonary Disease

Barbaro

Carpagnano

Spanevello

et al. 2007

Int J Immunopathol Pharmacol

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Chronic obstructive pulmonary diseases (COPD) is a pulmonary disease characterized by systemic abnormalities. The aim of this study is to investigate inflammation and systemic effects in mild COPD. Twenty-seven mild stable smoking related COPD patients and 15 age-matched healthy subjects were enrolled in the study. IL-6, TNF-a and IL-4 in plasma, sputum and exhaled breath condensate were measured. We also measured exhaled nitric oxide (NO) and pH in sputum and in breath condensate. Moreover, fat-free mass, body mass index (BMI), respiratory muscle strength, plasma oxidative stress and C-reactive protein (CRP) were measured. Higher concentrations were found of CRP, of diacron reactive oxygen metabolites (DROMs) and ofIL-6, TNF-a and IL-4 either in plasma or in supernatant of induced sputum or in exhaled breath condensate of COPD subjects compared to healthy controls. Furthermore, higher concentrations were observed of exhaled NO and lower exhaled pH in breath condensate of COPD when compared with healthy subjects. In the group of COPD patients, the subjects with airway reversibility showed an increase of sputum eosinophils and exhaled NO, whereas the subjects without airway obstruction reversibility showed an increase in sputum neutrophils, TNF-a and IL-6. We also found a trend towards a decrease in fat-free mass and respiratory muscle strength in COPD compared to healthy subjects and a negative correlation between these systemic indices (fatfree mass, maximal inspiratory pressure, maximal expiratory pressure) and TNF-a concentrations in the blood, sputum and breath condensate. We conclude that mild COPD subjects present an increase in inflammatory markers in blood and in airways of similar pattern and furthermore, the neutrophilic pattern of airway inflammation observed in the group of COPD subjects without an airway obstruction reversibility makes it more likely that systemic features are present.Chronic obstructive pulmonary disease (COPD) is usually considered to be a pulmonary disease (1). However, recent studies have demonstrated several extra-pulmonary abnormalities, particularly in more severely affected patients, that suggest the necessity of a wider definition of this disease (2). Several systemic features have been described in COPD patients, including systemic inflammation and oxidative stress, skeletal muscle dysfunction (SMD), weight loss, atherosclerosis and osteoporosis (3-5).

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Evidence of Lower Oxidative Stress in the Air Spaces of Patients with Reversible Copd

Cited by 6 publications

References 38 publications

Frailty Syndrome is Associated with Altered Circulating Redox Balance and Increased Markers of Oxidative Stress

Frailty Syndrome is Associated with Altered Circulating Redox Balance and Increased Markers of Oxidative Stress

Erdosteine enhances airway response to salbutamol in patients with mild-to-moderate COPD

Inflammation, Oxidative Stress and Systemic Effects in Mild Chronic Obstructive Pulmonary Disease

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