Evidence of mixed infection of phytoplasma and begomovirus associated with <i>Withania somnifera</i> and <i>Capsicum annum</i> plants from Uttar Pradesh, India

Tiwari, N. N.; Jain, Ravindra Kumar; Prajapati, Malyaj R.; Srivastava, Seweta; Tiwari, Ajay; Marcone, Carmine

doi:10.1080/03235408.2022.2156028

Cited by 1 publication

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“…Mixed infections of phytoplasmas and begomoviruses have been reported in different vegetable crops from other countries. These reports include: in tomato and pepper in Mexico (Cardenas-Conejo et al, 2010;Lebsky et al, 2011); in chickpea, eggplant, tomato, pepper and Withania somnifera in India (Swarnalatha and Reddy, 2014;Singh et al, 2015;Venkataravanappa et al, 2018;Reddy et al, 2021;Tiwari et al, 2022); in common bean in Cuba (Zamora et al, 2021); in tomato in Saudi Arabia (Sohrab et al, 2016); and in many ornamental crops (Ahmad and Khan, 2021). The present study results represent the first evidences of mixed infections of the whitefly-transmitted Begomovirus ToLCNDV and a 16SrI-B group related phytoplasma in diseased zucchini squash, and in Italy.…”

Section: Methodsmentioning

confidence: 99%

Mixed infections of Tomato yellow leaf curl New Delhi virus and a ‘Candidatus Phytoplasma asteris’ strain in zucchini squash in Italy

PARRELLA,

TROIANO

2024

Phytopathol. Mediterr.

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A new disease syndrome of zucchini squash was observed in Southern Italy, in 2018 and again in 2020. Affected plants were severely stunted and leaves were bent downwards, small, stiff, thick, leathery, and had interveinal chloroses. In addition, flowers were virescent and fruits were deformed and often cracked. Disease incidence was 20 and 30% in two different zucchini cultivations in Campania region (Southern Italy). Tomato yellow leaf curl New Delhi virus (ToLCNDV) was detected in eight samples, by loop-mediated isothermal amplification–based (LAMP) kit and by PCR and Sanger sequencing of the AV1 gene. Phytoplasmas were detected in the same samples using nested PCR assays with primer pairs P1/P7 and R16F2n/R16R2. Phytoplasma associations in plant samples were confirmed using specific primers for the multilocus genes SecY, tuf and rp. Sequence comparison of multilocus genes and phylogenetic analyses of the 16S rDNA gene confirmed the association of a phytoplasma strain closely related to ‘Candidiatus Phytoplamsa asteris’. This is the first report of mixed infections of ToLCNDV and a putative ‘Ca. Phytoplamsa asteris’ strain in zucchini, associated with a new Squash-Phytoplasma-Begomovirus (SqPB) disease syndrome.

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