Evidence of nickel ion requirement for autotrophic growth of a marine diatom with urea serving as nitrogen source

Oliveira, Luis; Antia, N. J.

doi:10.1080/00071618400650131

Cited by 35 publications

(15 citation statements)

References 41 publications

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“…2G) suggesting that Ni plays a similar role in the ureases of these species as in other bacteria. Addition of Ni is essential for the growth of coastal diatoms (Oliveira & Antia, 1984, 1986a,b; Egleston & Morel, 2008) and marine cyanobacteria Synechococcus (Dupont et al ., 2007) on urea as the sole N source. Urease activity has been shown to increase with rising Ni concentration (Mackerras & Smith, 1986; Price & Morel, 1991).…”

Section: Metals and N Assimilationmentioning

confidence: 99%

Coevolution of metal availability and nitrogen assimilation in cyanobacteria and algae

2009

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Marine primary producers adapted over eons to the changing chemistry of the oceans. Because a number of metalloenzymes are necessary for N assimilation, changes in the availability of transition metals posed a particular challenge to the supply of this critical nutrient that regulates marine biomass and productivity. Integrating recently developed geochemical, biochemical, and genetic evidence, we infer that the use of metals in N assimilation - particularly Fe and Mo - can be understood in terms of the history of metal availability through time. Anoxic, Fe-rich Archean oceans were conducive to the evolution of Fe-using enzymes that assimilate abiogenic NH(4)(+) and NO(2)(-). The N demands of an expanding biosphere were satisfied by the evolution of biological N(2) fixation, possibly utilizing only Fe. Trace O(2) in late Archean environments, and the eventual 'Great Oxidation Event' c. 2.3 Ga, mobilized metals such as Mo, enabling the evolution of Mo (or V)-based N(2) fixation and the Mo-dependent enzymes for NO(3)(-) assimilation and denitrification by prokaryotes. However, the subsequent onset of deep-sea euxinia, an increasingly-accepted idea, may have kept ocean Mo inventories low and depressed Fe, limiting the rate of N(2) fixation and the supply of fixed N. Eukaryotic ecosystems may have been particularly disadvantaged by N scarcity and the high Mo requirement of eukaryotic NO(3)(-) assimilation. Thorough ocean oxygenation in the Neoproterozoic led to Mo-rich oceans, possibly contributing to the proliferation of eukaryotes and thus the Cambrian explosion of metazoan life. These ideas can be tested by more intensive study of the metal requirements in N assimilation and the biological strategies for metal uptake, regulation, and storage.

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Section: Metals and N Assimilationmentioning

confidence: 99%

Coevolution of metal availability and nitrogen assimilation in cyanobacteria and algae

2009

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“…Urea hydrolysis can also be accomplished by adenosine triphosphate urea amidolyase, which does not contain Ni, but among marine phytoplankton this enzyme has only been found in chlorophytes [ Bekheet and Syrett , 1977; Dyhrman and Anderson , 2003]. Other phytoplankton growing on urea as the sole source of nitrogen have an obligate requirement for Ni [ Oliveira and Antia , 1984; Price and Morel , 1991]. Further, it is known that Ni‐containing superoxide dismutase (Ni‐SOD) is present in some marine prokaryotes [ Eitinger , 2004; Palenik et al , 2003], and this enzyme also appears to be present in oceanic diatoms [ Cuvelier et al , 2010].…”

Section: Introductionmentioning

confidence: 99%

Role of diatoms in nickel biogeochemistry in the ocean

Twining

Baines

Vogt

et al. 2012

Global Biogeochemical Cycles

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[1] Dissolved nickel (Ni) typically displays a 'nutrient-like' vertical profile in the ocean, with lower concentrations in surface waters and higher concentrations in deep waters, similar to other micronutrient metals such as iron and zinc. Vertical profiles of Ni show particular similarities to profiles of the macronutrients phosphate and silicic acid, suggesting that diatoms play an important role in mediating the vertical distribution of this metal. We performed synchrotron x-ray fluorescence (SXRF) analysis on individual phytoplankton cells collected from stations in the equatorial Pacific Ocean and from nutrient-addition incubation experiments conducted on the same cruise. Diatoms were enriched in Ni twofold to fivefold relative to picoplankton and flagellated cells. Changes in cellular quotas of Si, P and Ni observed in diatoms growing in response to Fe and Si additions were used to estimate the Ni:P (0.52 AE 0.10 mmol/mol) and Ni:Si (28 AE 13 mmol/mol) ratios of internal biomass and the frustule, respectively. Elevated internal Ni:P suggests a heightened role for urease or the Ni isoform of superoxide dismutase in diatoms (similar to cyanobacteria), while Ni associated with the frustule appears to contribute an additional 50% of cellular Ni found in the diatoms. The derived Ni:Si ratio for frustule material is comparable to Ni:Si ratios in published nutrient profiles, confirming the dominant role that diatoms play in ocean Ni biogeochemistry. While a molecular explanation for the association of Ni with frustules remains to be determined, this study demonstrates the unique biogeochemical insight that can be gained from microanalytical element analysis.

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“…Ureases from other sources have also been shown to be Nittdependent (10). However, the algal Ureases are less well studied, but the dependence of urease containing algae on the presence of Ni2+ in the medium when they are growing on urea indicates strongly that Ni2+ is a costituents of algal urease (3,4,7,8,12,14). In contrast, Van Baalen and O'Donnell (16) isolated a species of Oscillatoria which exhibited the absolute requirement of Ni2+ when grown in a medium in which NH4 was the sole nitrogen source.…”

mentioning

confidence: 99%

Role of nickel and N-starvation in the regulation of urea metabolism in the cyanobacterium Anacystis nidulans.

Singh

1991

J. Gen. Appl. Microbiol.

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Evidence of nickel ion requirement for autotrophic growth of a marine diatom with urea serving as nitrogen source

Cited by 35 publications

References 41 publications

Coevolution of metal availability and nitrogen assimilation in cyanobacteria and algae

Coevolution of metal availability and nitrogen assimilation in cyanobacteria and algae

Role of diatoms in nickel biogeochemistry in the ocean

Role of nickel and N-starvation in the regulation of urea metabolism in the cyanobacterium Anacystis nidulans.

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