Evidence of post-transcriptional readthrough regulation in FGF5 gene of alpaca

Pallotti, Stefano; Pediconi, Dario; Dharaneedharan, Subramanian; Molina, Gabriela; Antonini, Marco; Morelli, Maria Beatrice; Renieri, Carlo; Terza, Antonietta La

doi:10.1016/j.gene.2018.01.006

Cited by 14 publications

(23 citation statements)

References 47 publications

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“…C4orf22 is expressed in human neural progenitor cells expressing MEF2CA [70]. Functional analysis of the FGF5 gene in Suri and Huacaya alpacas has previously shown its potential importance in regulating fleece length [71], and preliminary analysis of expression profiles in domestic SACs has shown the potential importance of a novel QTL for fine fiber production in merino sheep [23]. Further studies should focus on investigating these genes and the QTL they underpin.…”

Section: Discussionmentioning

confidence: 99%

Genomic analysis of the domestication and post-Spanish conquest evolution of the llama and alpaca

Fan

Guang

et al. 2020

Genome Biol

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Background: Despite their regional economic importance and being increasingly reared globally, the origins and evolution of the llama and alpaca remain poorly understood. Here we report reference genomes for the llama, and for the guanaco and vicuña (their putative wild progenitors), compare these with the published alpaca genome, and resequence seven individuals of all four species to better understand domestication and introgression between the llama and alpaca. Results: Phylogenomic analysis confirms that the llama was domesticated from the guanaco and the alpaca from the vicuña. Introgression was much higher in the alpaca genome (36%) than the llama (5%) and could be dated close to the time of the Spanish conquest, approximately 500 years ago. Introgression patterns are at their most variable on the X-chromosome of the alpaca, featuring 53 genes known to have deleterious X-linked phenotypes in humans. Strong genome-wide introgression signatures include olfactory receptor complexes into both species, hypertension resistance into alpaca, and fleece/fiber traits into llama. Genomic signatures of domestication in the llama include male reproductive traits, while in alpaca feature fleece characteristics, olfaction-related and hypoxia adaptation traits. Expression analysis of the introgressed region that is syntenic to human HSA4q21, a gene cluster previously associated with hypertension in humans under hypoxic conditions, shows a previously undocumented role for PRDM8 downregulation as a potential transcriptional regulation mechanism, analogous to that previously reported at high altitude for hypoxia-inducible factor 1α. Conclusions: The unprecedented introgression signatures within both domestic camelid genomes may reflect post-conquest changes in agriculture and the breakdown of traditional management practices.

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Section: Discussionmentioning

confidence: 99%

Genomic analysis of the domestication and post-Spanish conquest evolution of the llama and alpaca

Fan

Guang

et al. 2020

Genome Biol

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“…The FGF5 gene participates in the FGF pathway, which plays a central role in hair growth. Studies on the FGF5 gene demonstrated the relation to coat hair length in mice [50], dogs [51], cats [52], humans [53], donkeys [54] and alpacas [55]. The same selection target has been described in a number of cashmere goat investigations [10,56].…”

Section: Discussionmentioning

confidence: 69%

“…In addition, disruption of the FGF5 gene via the CRISPR/Cas9 system in cashmere goats increased the number of secondary hair follicles and enhanced the ber length [57]. Previous studies have found a few SNP variants of the FGF5 gene that may be associated with hair length, including a missense SNP (c.284G> T) in dogs, four SNPs (c.194C>A, c.182T>A, c.474delT and c.475A>C) in cats, two SNPs (c.433_434delAT and c.245G> A) in donkeys and a missense SNP (c.499C>T) in alpacas [51,52,54,55]. Recently, one SNP (c.253G>A) in the 5'-UTR of FGF5 resulted in a start codon that could lead to a premature/dysfunctional protein in Tibetan cashmere goats [56].…”

Section: Discussionmentioning

confidence: 99%

Deletion of an Enhancer in FGF5 is Associated With Ectopic Expression in Goat Hair Follicles and the Cashmere Growth Phenotype

Song

Liu

et al. 2020

Preprint

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BackgroundResearch on cashmere growth has a significant effect on the production of cashmere and a profound influence on cashmere goat breeding. Whole-genome sequencing is a powerful platform to rapidly gain novel insights into the identification of genetic mechanisms underlying cashmere fiber growth. ResultsIn this study, we generated whole-genome sequences of 115 domestic goats from China, Nepal and Pakistan, including 51 cashmere goats and 64 non-cashmere goats. We found genetically distinct clusters according to their geographic locations but genetic admixture or introgression may have occurred between the Chinese and Nepalese goats. We identified that the fibroblast growth factor 5 gene (FGF5) shows a strong signature for positive selection in the cashmere goat. The 505-bp indel variant at the FGF5 gene locus appeared to be strongly associated with cashmere growth. Functional validation showed that the insertion variant may serve as an enhancer for transcription factor binding, resulting in increased transcription of the upstream FGF5 gene in non-cashmere goats. ConclusionsOur study provides useful information for the sustainable utilization and improved conservation of goat genetic resources and demonstrates that the indel mutation in the FGF5 gene could potentially serve as a molecular marker of cashmere growth in cashmere goat breeding.

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“…The FGF5 gene is also a determinant of the long hair phenotype in domestic cats. The long subtype of the FGF5 gene in alpacas prematurely produces a point mutation in the stop codon (PTC), leading to an increase in alpaca hair length [12]. Steven Krege found that Sox2 can regenerate hair follicle cells in the skin through FGF5 [13][14].…”

Section: Introductionmentioning

confidence: 99%

Screening and Identification of LncRNAs Related to Villus Growth of Liaoning Cashmere Goats and Their Effects on Growth after FGF5 Treatment

Jin

Zhao

et al. 2020

Preprint

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(Background) Liaoning Cashmere Goat cashmere has high economic value FGF5 is an important factor regulating its growth. The role of long non-coding RNA (LncRNA) in the mammalian villus growth cycle has still not been studied in detail.(Results) We demonstrated that treatment of skin cells with FGF5 inhibited the expression of LncRNA in cells, down-regulated the expression of the target genes CBS and CTH, and promoted the expression of related keratin genes k26, kap11.1. Overexpressing LncRNA reversed the inhibiting effect of FGF5 on the target genes CBS and CTH. (Conclusions) we believe that FGF5 can regulate the growth and development of Cashmere Goat hair by promoting the expression of related keratin and keratin-associated protein genes. This mechanism is achieved by inhibiting the expression of the LncRNA gene and then down-regulating the expression of the target genes CBS and CTH.

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Evidence of post-transcriptional readthrough regulation in FGF5 gene of alpaca

Cited by 14 publications

References 47 publications

Genomic analysis of the domestication and post-Spanish conquest evolution of the llama and alpaca

Genomic analysis of the domestication and post-Spanish conquest evolution of the llama and alpaca

Deletion of an Enhancer in FGF5 is Associated With Ectopic Expression in Goat Hair Follicles and the Cashmere Growth Phenotype

Screening and Identification of LncRNAs Related to Villus Growth of Liaoning Cashmere Goats and Their Effects on Growth after FGF5 Treatment

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