Evidence of putative sensory receptors from snout and tongue in an upstream amphihaline migratory fish hilsa Tenualosa ilisha

Journal of Fish Biology

et al. 2018

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The chemosensory nature of the tissue from the dorsal surface of the head (also termed sensory pad; SP) of the amphihaline diadromous fish hilsa Tenualosa ilisha was investigated for odorant receptor (OR), olfactory marker protein (OMP) and G‐protein subunits (Gαs‐olf, Gαq, Gαo, Gαi3) through immunolocalization and immunoblotting techniques. The immunolocalization of OR, OMP and G‐protein subunits showed clear expression of these proteins in the tissues of the SP. Robust expressions of these proteins in the SP were detected with immunoblot analysis. The strong expression of these proteins in the SP indicates that the tissues from this area in riverine T. ilisha may play significant role in chemosensing and signalling through ectopic expression of olfactory receptor proteins which are otherwise reported in olfactory organs in vertebrates. Being migratory in nature, ectopic expression of these receptors in T. ilisha probably helps them to prevent damage to epidermal tissues of the SP, or they may also utilize them as a chemo and mechanosensory tool to optimize chemo‐communications during migration.

Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ectopic expression of olfactory receptors and associated G‐protein subunits in the head integument of the amphihaline migratory fish hilsa Tenualosa ilisha

Journal of Fish Biology

et al. 2018

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“…The knowledge on various sensory cells in the olfactory epithelium (OE) and their functional aspects in regard to migration may be important for various reasons, like the maintenance of breeding grounds, habitat management, and to conserve this little known migratory fish species. Very recently, Malick et al () reported on the putative chemoreceptors of hilsa from snout and tongue in relation to migration. The present article discusses another important attributor of migration, the olfactory organ, and expression of G protein subunits in relation to the migration biology of the fish.…”

Section: Introductionmentioning

confidence: 99%

Structural organization of the olfactory organ in an amphihaline migratory fish Hilsa, Tenualosa ilisha

Microscopy Res & Technique

et al. 2018

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The histological as well as ultramicroscopic structures of olfactory system of an amphihaline migratory fish hilsa Tenualosa ilisha, were studied. The sexually matured riverine fish were collected from a common breeding habitat—the Hooghly, a tributary of river Ganga, West Bengal, India. This study revealed that the riverine hilsa has larger olfactory bulb compared to marine hilsa with the olfactory lobes well exposed through nostrils. The olfactory lamellae (OL) are 40–45 in number and posses three distinct layers of sensory cells across each lamellae, namely, outer receptor cells (RC), middle sensory cells, and inner basal cells (BC). Besides the above arrangement, the sensory part of olfactory epithelium (OE) also bears rich microvillous cells exposed to the surface of the OE. The sensory and non‐sensory surfaces on OL are distinguishable, with clear dendritic cells on sensory epithelium and solitary chemosensory cells on non sensory OE. Abundance of both types of cells in the OE is an indication of its chemoattraction ability towards molecules of amino acid origin. The feature of having abundant, dense, and large dendritic knobs on the surface of OE describes resemblance to the typical morphology of the chemosensory septal organs neuron. The expression of four G protein subunits, like Gαs/olf, Gαq, Gαo, and Gαi‐3 in OE indicate that its olfaction is a functional attributes of two olfactory systems, namely main olfactory system and Vomaronasal Olfactory System. Expression of ACIII and PLCβ2 in OE further confirms two signaling pathways involved in odorant reception in hilsa. Research Highlights The olfactory bulb in the amphihaline migratory fish hilsa is big in size, with 40–45 lamellae. Its sensory areas showed multilayered cellular features with prominent sensory as well as microvillous cells, whereas non‐sensory area possesses solitary chemosensory cells. The expression of four G protein subunits, Gαs/olf, Gαq, Gαo, and Gαi‐3 in olfactory epithelium indicates that its olfaction is a functional attributes of two olfactory systems, namely main olfactory system and vomaronasal olfactory system.

Gustatory ultrastructures of an amphihaline migratory fish hilsaTenualosa ilisha

Microscopy Res & Technique

et al. 2020

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