Evidence of queen-rearing suppression by mature queens in the little fire ant, Wasmannia auropunctata

Abstract

The little fire ant (LFA), Wasmannia auropunctata , is a serious invasive pest first reported on Hawaii Island in 1999, and has since spread and established itself across the island. LFA is considered one of the worst 100 invasive species and has significant ecological, agricultural, and public health impacts in invaded areas, which include much of the tropical New World. Although localized eradication efforts have proven successful, they are intensive and difficult to implement. Furthermore, LFA’s high invasive-ability resists these control efforts in areas where the species is established and can re-infest treated areas. This research set out to determine whether LFA queens have a suppressant effect on new queen production in nests, as a first step in identifying a potential queen pheromone for LFA. A queen pheromone could offer a means to shutdown LFA reproductive capability, potentially by suppressing the production of new queens or inducing the execution of queens or queen-destined larvae. When queenless experimental nests and polygyne experimental nests were compared, six out of eight queenless nests successfully reared both new alate queens (2.25 queens/nest) and drones (3.63 drones/nest) to adulthood, whereas only three of eight polygyne nests reared sexual larvae that failed to develop to adulthood or even the pupal stage. These results suggest that dealate mature LFA queens suppress the production of new alate queens in LFA nests, and is the first evidence that LFA may utilize a queen pheromone.