Evidence of reproductive isolation among closely related sympatric species of <i>Serrasalmus</i> (Ostariophysii, Characidae) from the Upper Madeira River, Amazon, Bolivia

Hubert, Nicolas; Duponchelle, Fabrice; Nunez, Jésus; Rivera, Rosario; Renno, Jean‐François

doi:10.1111/j.1095-8649.2006.01097.x

Cited by 19 publications

(18 citation statements)

References 46 publications

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“…Polymorphism at the seven intronic loci was previously detected within Serrasalmus rhombeus , and one to two polymorphic loci were obtained per primer pair with a diversity ranging from two to four alleles per locus, as previously reported (Hubert et al . 2006).…”

Section: Resultssupporting

confidence: 62%

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Isolation by distance and Pleistocene expansion of the lowland populations of the white piranha Serrasalmus rhombeus

et al. 2007

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The genetic variability and distribution of Amazonian fish species have likely been influenced by major disturbance events in recent geological times. Alternatively, the great diversity of aquatic habitat in the Amazon is likely to shape ongoing gene flow and genetic diversity. In this context, complex patterns of genetic structure originating from a joint influence of historical and contemporary gene flow are to be expected. We explored the relative influence of Pleistocene climatic fluctuations and current water chemistry on the genetic structure of a piranha, Serrasalmus rhombeus, in the Upper Amazon by the simultaneous analysis of intron length polymorphism and mitochondrial DNA sequences. The Madeira river is well suited for that purpose as it is characterized by a great diversity of water types, the presence of one of the largest floodplain of the Amazon and the potential occurrence of two Pleistocene refuges. We found evidence of genetic structure even at a small geographical scale (less than 10 km), indicating that the floodplain is not a homogenizing factor promoting interdrainage dispersal in S. rhombeus. Likewise, the hierarchical genetic structure inferred was correlated to geographical distance instead of habitat characteristic. Our results also support the hypothesis that the area underwent population expansion during the last 800,000 years. In addition, a higher level of genetic diversity was found in the samples from the putative Aripuanã refuge. The present findings suggest that Pleistocene refuges contributed significantly to the colonization of the lowlands in the Upper Amazon valley during the Pleistocene.

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Section: Resultssupporting

confidence: 62%

“…2004). Recently, several intron loci were scored among the Serrasalmus species of the Madeira for molecular systematic purposes with several polymorphic loci detected for S. rhombeus in the area (Hubert et al . 2006).…”

Section: Methodsmentioning

confidence: 99%

Isolation by distance and Pleistocene expansion of the lowland populations of the white piranha Serrasalmus rhombeus

et al. 2007

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“…The taxonomy of S. spilopleura and S. eigenmanni has been complex due to the lack of confident geographical records and diagnostic characters (Jégu & Dos Santos 2001). Hubert et al . (2006) recently provided evidence that the citation of S. eigenmanni in the Madeira was erroneous and originated from confusion with S. spilopleura .…”

Section: Resultsmentioning

confidence: 99%

“…1992; Nakayama et al . 2002; Hubert et al . 2006); (ii) they constitute a clade of freshwater fishes endemic to South America (Orti et al .…”

Section: Introductionmentioning

confidence: 99%

Phylogeography of the piranha genera Serrasalmus and Pygocentrus: implications for the diversification of the Neotropical ichthyofauna

et al. 2007

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The phylogenetic relationships within the piranhas were assessed using mitochondrial sequences with the aim of testing several hypotheses proposed to explain the origin of Neotropical diversity (palaeogeography, hydrogeology and museum hypotheses). Sequences of the ribosomal 16S gene (510 bp) and control region (980 bp) were obtained from 15 localities throughout the main South American rivers for 21 of the 28 extant piranha species. The results indicate that the genus Serrasalmus is monophyletic and comprises three major clades. The phylogeographical analyses of these clades allowed the identification of five vicariant events, extensive dispersal and four lineage duplications suggesting the occurrence of sympatric speciation. Biogeographical patterns are consistent with the prediction made by the museum hypothesis that lineages from the Precambrian shields are older than those from the lowlands of the Amazon. The vicariant events inferred here match the distribution of the palaeoarches and several postdispersal speciation events are identified, thereby matching the predictions of the palaeogeography and hydrogeology hypotheses, respectively. Molecular clock calibration of the control region sequences indicates that the main lineages differentiated from their most recent common ancestor at 9 million years ago in the proto Amazon-Orinoco and the present rate of diversification is the highest reported to date for large carnivorous Characiformes. The present results emphasize that an interaction among geology, sea-level changes, and hydrography created opportunities for cladogenesis in the piranhas at different temporal and geographical scales.

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“…For example, ILPs were applied to characterize Mytilus spp. populations and hybridization events between Mytilus edulis and Mytilus galloprovincialis (Daguin et al 2001) and were recommended for taxonomic studies in the fish species Serrasalmus (Hubert et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Intron polymorphism pattern in AOX1b of wild St John's wort (Hypericum perforatum) allows discrimination between individual plants

Ferreira¹,

Cardoso²,

Macedo³

et al. 2009

Physiologia Plantarum

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The present paper deals with the analysis of natural polymorphism in a selected alternative oxidase (AOX) gene of the medicinal plant, St John's wort. Four partial AOX gene sequences were isolated from the genomic DNA of a wild plant of Hypericum perforatum L. Three genes belong to the subfamily AOX1 (HpAOX1a, b and c) and one to the subfamily AOX2 (HpAOX2). The partial sequence of HpAOX1b showed polymerase chain reaction (PCR) fragment size variation as a result of variable lengths in two introns. PCR performed by Exon Primed Intron Crossing (EPIC)-PCR displayed the same two-band pattern in six plants from a collection. Both fragments showed identical sequences for all exons. However, each of the two introns showed an insertion/deletion (InDel) in identical positions for all plants that counted for the difference in the two fragment sizes. The InDel in intron 1 influenced the predictability of a pre-microRNA site. The almost identical PCR fragment pattern was characterized by a high variability in the sequences. The InDels in both introns were linked to repetitive intron single nucleotide polymorphisms (ISNP)s. The polymorphic pattern obtained by InDels and ISNPs from both fragments together was appropriate to discriminate between all individual plants. We suggest that AOX sequence polymorphism in H. perforatum can be used for studies on gene diversity and biodiversity. Further, we conclude that AOX sequence polymorphism of individual plants should be considered in biological studies on AOX activity to exclude the influence of genetic diversity. The identified polymorphic fragments are available to be explored in future experiments as a potential source for functional marker development related to the characterization of origins/accessions and agronomic traits such as plant growth, development and yield stability.

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Evidence of reproductive isolation among closely related sympatric species of Serrasalmus (Ostariophysii, Characidae) from the Upper Madeira River, Amazon, Bolivia

Cited by 19 publications

References 46 publications

Isolation by distance and Pleistocene expansion of the lowland populations of the white piranha Serrasalmus rhombeus

Isolation by distance and Pleistocene expansion of the lowland populations of the white piranha Serrasalmus rhombeus

Phylogeography of the piranha genera Serrasalmus and Pygocentrus: implications for the diversification of the Neotropical ichthyofauna

Intron polymorphism pattern in AOX1b of wild St John's wort (Hypericum perforatum) allows discrimination between individual plants

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