Evidence of synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon

Fondong, Vincent N.; Pita, Justin S.; Rey, M. E. C.; Kochko, Alexandre de; Beachy, Roger N.; Fauquet, Claude

doi:10.1099/0022-1317-81-1-287

Cited by 271 publications

(241 citation statements)

References 24 publications

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“…These differences were not very pronounced, and the general disease situation was of relatively severe disease when comparing data with averages for other countries (Sseruwagi et al, 2004b). This could be attributed to the high frequency of occurrence of mixed virus infections, known to elicit more severe symptoms through synergistic interactions Fondong et al, 2000;Pita et al, 2001). Although ACMV was the most frequently occurring virus in both years, the greatest proportion of sampled diseased plants had mixed ACMV+EACMV-UG infections.…”

Section: Discussionmentioning

confidence: 81%

“…Effects on yield vary from no reduction to total yield loss, depending on cassava variety and environmental conditions (Muimba-Kankolongo andPhuti, 1987, OtimNape et al, 1993). Dual infections of EACMV and ACMV or EACMV-UG and ACMV commonly lead to synergistic interactions resulting in more severe symptoms Fondong et al, 2000 ). Losses of 80-90% have been reported for plants infected by both ACMV and EACMV-UG in Uganda (Owor et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Incidence and severity of cassava mosaic disease in the Republic of Congo

Ntawuruhunga¹,

Okao-Okuja²,

Bembe³

et al. 2010

African Crop Science Journal

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Diagnostic surveys were conducted in 2002 and 2003 in order to provide a comprehensive and detailed assessment of the status of cassava mosaic disease (CMD) in the Republic of Congo (ROC) and to determine if the disease was spreading. In 2002, 105 farmers' fields were assessed in the four major cassava-producing regions of the country. In 2003, 163 fields were sampled in nine regions and Brazzaville Commune. Incidence of cassava mosaic disease was generally high, averaging 80 and 86 % for 2002 and 2003, respectively; while damage was moderate to severe. In 2002, disease incidence was moderate in Pool (73%) but high in Brazzaville (81%), Cuvette Centrale (82%) and in Plateaux Region (84%). Pool region still had the lowest incidence (78%) in 2003, while Sangha (95%) had the highest incidence. The greatest disease severity was recorded in Niari region in the south and Cuvette Ouest region in the north in 2003. East African cassava mosaic virus Uganda variant (EACMV-UG) occurred virtually throughout the country, commonly in dual infections with African cassava mosaic virus. The high incidence of disease in plants considered to be the results of cutting infection (74% in 2002 and 82% in 2003), relatively low incidence of disease in plants considered to be infected by whiteflies and wide distribution of EACMV-UG points to the fact that the CMD pandemic is a chronic in the country and the areas sampled are currently in a stable post-epidemic phase. This situation is comparable to that in areas of East Africa affected by a pandemic during the 1990s, including Uganda, parts of western Kenya and north-western Tanzania. These findings clearly verify the assertion that the CMD pendemic has expanded across Central Africa and provide a basis for designing interventions and control strategies for the entire region. dans la région du Pool demeurait encore plus faible (78%) en 2003 pendant que Sangha avait l'incidence plus élevée (95%). En 2003 la plus grande gravité de la maladie était enregistrée dans la région de Niari au Sud et dans la région Ouest de la cuvette au Nord. La variante du virus de la mosaïque du manioc de l'Afrique de l'Est en Ouganda (VVMMAEO) s'était virtuellement manifestée à travers le pays; les infections s'étaient couplées avec le virus de la mosaïque du manioc africain. La grande incidence de la maladie des plantes considérée être les résultats de la contagion des boutures était de 74% en 2002. Relativement, la basse incidence dans les plantes considérées être contaminées par les mouches blanches et la grande part du VVMMAEO était de 82% en 2003. Ceci a abouti à la conclusion selon laquelle la pandémie de la MMM avait contaminé le pays quelques années auparavant et que les régions ayant fait l'objet de l'échantillon sont actuellement dans une phase post-endémique. Cette situation est comparable à celle des régions de l'Afrique de l'Est qui étaient contaminées par une pandémie les années 1990 y compris l'Ouganda, les parties Ouest du Kenya et le Nord-Ouest de la Tanzanie.

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Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Incidence and severity of cassava mosaic disease in the Republic of Congo

Ntawuruhunga¹,

Okao-Okuja²,

Bembe³

et al. 2010

African Crop Science Journal

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“…Total DNA was extracted from leaves stored in a silica gel as described (Alabi et al, 2008) and used as a template for rollingcircle amplification (RCA) of complete begomovirus genomes as per Illustra TempliPhi amplification kit (GE Healthcare Life Sciences, UK). The RCA products were first PCR-amplified using begomovirus universal primer pair EBB555F /R1 (Fondong et al, 2000) to see if they contain any begomovirus infection, and subsequently used to construct Illumina libraries and sequenced at North Carolina State Genomic Sciences Laboratory (Raleigh, NC, USA) by next generation sequencing.…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic characterization of East African cassava mosaic begomovirus (Geminiviridae) isolated from Manihot carthaginensis subsp. glaziovii (Mll.Arg.) Allem., from a non-cassava growing region in Tanzania

Tairo

Mbewe

Mark

et al. 2017

Afr. J. Biotechnol.

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Manihot carthaginensis subsp. glaziovii (Müll.Arg.) Allem., a wild relative of cassava, native to Brazil, is one of the popular agroforestry trees used for hedges and/or boundary plants surrounding homesteads and farms and also harbours cassava mosaic begomoviruses (CMBs) and cassava brown streak ipomoviruses. Sequences of the DNA-A component of East African cassava mosaic virus (EACMV) isolates from M. carthaginensis subsp. glaziovii (Müll.Arg.) Allem., collected from non-cassava growing areas of Tanzania were characterized. Thirteen full length DNA-A sequences were analysed together with 15 already reported EACMV sequences and six CMB species reference genomes. The results show 96 to 100% nucleotide sequence identity with EACMV isolates from Kenya. Phylogenetic analysis revealed that EACMV isolates from M. carthaginensis subsp. glaziovii (Müll.Arg.) Allem, belong to a single cassava mosaic begomovirus species. The EACMV monophyletic clade is distinct from all other CMB species. The presence of Cassava infecting begomoviruses in wild cassava relative growing from traditionally non cassava growing region serve as inoculum sources for cassava-infecting begomoviruses and therefore their eradication is key in the sustainable management of CMBs, especially in the non-cassava growing areas.

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“…Plant RNA viruses are more genetically stable than animal RNA viruses but geminiviruses evolve faster than some plant RNA viruses, including tobamoviruse and criniviruses (Duffy et al, 2008). Recombination events in geminiviruses with single-stranded DNA (ssDNA) genomes have been previously reported in a number of viruses, including studies of the African cassava mosaic virus (Fondong et al, 2000), East African cassava mosaic virus (Zhou et al, 1997), Cotton leaf curl virus (Zhou et al, 1998), Pepper huasteco virus (Bonilla-Ramirez et al, 1997;Torres-Pacheco et al, 1993), and Tomato leaf curl virus (ToLCV) (Umaharan et al, 1998). These recombination studies have spurred greater interest in the relationships between the frequency and role of recombination events and the microevolution of new geminiviruses.…”

mentioning

confidence: 99%

“…This process occurs via four major processes: recombination, mutation, reassortment, and de novo gene acquisition (Padidam et al, 1999). Natural recombination has been shown to occur in both RNA and DNA genomes and performs important functions in the evolution of animal and plant viruses at the strain, species, genus, and family levels (Briddon et al, 1996;Fondong et al, 2000;Klute et al, 1996;Sanz et al, 2000;Saunders and Stanley, 1999). Plant RNA viruses are more genetically stable than animal RNA viruses but geminiviruses evolve faster than some plant RNA viruses, including tobamoviruse and criniviruses (Duffy et al, 2008).…”

mentioning

confidence: 99%

Molecular Evidence of Recombination on Korean Isolates of Tomato yellow leaf curl virus by Nucleotide Transversions and Transitions

Lee¹,

Park²,

Auh³

et al. 2011

The Plant Pathology Journal

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Tomato yellow leaf curl virus (TYLCV), a member of genus Begomovirus, was isolated in Korea in 2008. We sequenced and analyzed the DNA-A of 51 TYLCV isolates from Korea, and 13 of the TYLCV isolates were selected as type representatives of TYLCV from six Korean provinces. The 13 TYLCV isolates were classified into Korea Group 1 (KG1, nine isolates) and Korea Group 2 (KG2, four isolates) based on the results of phylogenetic analysis and genome size (2774 and 2781 nucleotides, respectively). A recombination detection program 3 (RDP3) revealed two recombinations between the TYLCV Korea isolates and other TYLCV isolates [Thailand (AF206674), Iran (AJ132711), and Israel (X76319)]. TYLCV Jeju isolate was characterized by two recombination events (E1 and E2) caused by the presence of E1 in ORF V1 and C3, which may seem to be the mutations of the high nucleotide transversion and transition rate. Collectively, our results suggest that the occurrence of nucleotide transversions and transitions in TYLCV DNA-A might have induced novel recombination events within the TYLCV Korea isolates.

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Evidence of synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon

Cited by 271 publications

References 24 publications

Incidence and severity of cassava mosaic disease in the Republic of Congo

Incidence and severity of cassava mosaic disease in the Republic of Congo

Phylogenetic characterization of East African cassava mosaic begomovirus (Geminiviridae) isolated from Manihot carthaginensis subsp. glaziovii (Mll.Arg.) Allem., from a non-cassava growing region in Tanzania

Molecular Evidence of Recombination on Korean Isolates of Tomato yellow leaf curl virus by Nucleotide Transversions and Transitions

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