Evidence of Trypanosoma cruzi II infection in Colombian chagasic patients

Zafra, German; Mantilla, Julio César; Valadares, Helder Magno Silva; Macedo, Andréa Mara; González, Clara Isabel

doi:10.1007/s00436-008-1034-0

Cited by 51 publications

(46 citation statements)

References 18 publications

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“…Our results showed that using two molecular markers was not sufficient for typing of T. cruzi isolates. These limitations were also observed by other authors 32,[60][61][62][63] . However, the use of three genetic markers with different evolutionary rates was able to ensure the correct typing of T. cruzi isolates.…”

Section: Discussionsupporting

confidence: 63%

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Volpato

Sousa

D’Ávila

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:In order to detect Trypanosoma cruzi and determine the genetic profiles of the parasite during the chronic phase of Chagas disease (ChD), parasitological and molecular diagnostic methods were used to assess the blood of 91 patients without specific prior treatment. Methods: Blood samples were collected from 68 patients with cardiac ChD and 23 patients with an indeterminate form of ChD, followed by evaluation using blood culture and polymerase chain reaction. T. cruzi isolates were genotyped using three different genetic markers. Results: Blood culture was positive in 54.9% of all patients, among which 60.3% had the cardiac form of ChD, and 39.1% the indeterminate form of ChD. There were no significant differences in blood culture positivity among patients with cardiac and indeterminate forms. Additionally, patient age and clinical forms did not influence blood culture results. Polymerase chain reaction (PCR) was positive in 98.9% of patients, although comparisons between blood culture and PCR results showed that the two techniques did not agree. Forty-two T. cruzi stocks were isolated, and TcII was detected in 95.2% of isolates. Additionally, one isolate corresponded to TcIII or TcIV, and another corresponded to TcV or TcVI. Conclusions: Blood culture and PCR were both effective for identifying T. cruzi using a single blood sample, and their association did not improve parasite detection. However, we were not able to establish an association between the clinical form of ChD and the genetic profile of the parasite.

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Section: Discussionsupporting

confidence: 63%

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Volpato

Sousa

D’Ávila

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…In humans infected with T. cruzi, 60-70% never develop symptoms of infection, whereas the other 30-40% will develop symptoms that manifest themselves in one of at least three general ways (cardiac, digestive, or both), 1,13 and this is known to vary by region, with the southern cone of South America being associated with gastrointestinal problems and in the northern parts of South America associated with heart failure. 85 These disease variations have been linked to the different T. cruzi genotypes circulating in these areas. 5,8 T. cruzi parasitemia in mice and opossums has been found to vary with T. cruzi strain and clone, 12,86-89 tissue lesions caused by T. cruzi have been found to be different between sylvatic and domestic animals, 13 and differential tissue distribution was observed between different clones of T. cruzi when infecting mice.…”

Section: Discussionmentioning

confidence: 99%

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Peterson

Graham

Dobson

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The effect of a parasite on the life history of its vector is important for understanding and predicting disease transmission. Chagas disease agent Trypanosoma cruzi is a generalist parasite that is diverse across scales from its genetic diversity to the 100s of mammal and vector species it infects. Its vertebrate hosts show quite variable responses to infection, however, to date there are no studies looking at how T. cruzi variability might result in variable outcomes in its invertebrate host. Therefore, we investigated the effect of different T. cruzi I strains on Rhodnius prolixus survival and development. We found significant variation between insects infected with different strains, with some strains having no effect, as compared with uninfected insects, and others with significantly lower survival and development. We also found that different variables had varying importance between strains, with the effect of time postinfection and the blood:weight ratio of the infective meal significantly affecting the survival of insects infected with some strains, but not others. Our results suggest that T. cruzi can be pathogenic not only to its vertebrate hosts but also to its invertebrate hosts.

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“…Also, the influence of the stDNA copy number difference between the T. cruzi lineages has been established (12,15,24), where the number of stDNA copies in T. cruzi I is lower than the number in T. cruzi II. It is important to mention that parasites circulating in Colombia belong to T. cruzi I but there is evidence of patients infected with T. cruzi II parasites (26,46). This fact could affect the sensitivity of the PCR due to changes in the sequence and copy number seen in the parasites according to the primers used.…”

Section: Discussionmentioning

confidence: 99%

“…This parasite can be genetically classified into two major lineages, namely, T. cruzi I, which is found in northern South American countries, and T. cruzi II, which is found in southern South American countries (46). Chagas' disease is a chronic systemic disease endemic to both South and Central America.…”

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confidence: 99%

Evaluation of Adult Chronic Chagas' Heart Disease Diagnosis by Molecular and Serological Methods

et al. 2009

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Chagas' disease caused by Trypanosoma cruzi is endemic in Latin America. T. cruzi presents heterogeneous populations and comprises two main genetic lineages, named T. cruzi I and T. cruzi II. Diagnosis in the chronic phase is based on conventional serological tests, including indirect immunofluorescence (IIF) and enzymelinked immunosorbent assay (ELISA), and diagnosis in the acute phase based on parasitological methods, including hemoculture. The objective of this study was to evaluate the diagnostic procedures of Chagas' disease in adult patients in the chronic phase by using a PCR assay and conventional serological tests, including TESA-blot as the gold standard. Samples were obtained from 240 clinical chronic chagasic patients. The sensitivities, compared to that of TESA-blot, were 70% for PCR using the kinetoplast region, 75% for PCR using the nuclear repetitive region, 99% for IIF, and 95% for ELISA. According to the serological tests results, we recommend that researchers assess the reliability and sensitivity of the commercial kit Chagatest ELISA recombinant, version 3.0 (Chagatest Rec v3.0; Wiener Lab, Rosario, Argentina), due to the lack of sensitivity. Based on our analysis, we concluded that PCR cannot be validated as a conventional diagnostic technique for Chagas' disease. These data have been corroborated by low levels of concordance with serology test results. It is recommended that PCR be used only for alternative diagnostic support. Using the nuclear repetitive region of T. cruzi, PCR could also be applicable for monitoring patients receiving etiologic treatment.

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Evidence of Trypanosoma cruzi II infection in Colombian chagasic patients

Abstract: Trypanosoma cruzi is genetically classified into at least two major lineages named T. cruzi

Cited by 51 publications

References 18 publications

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Evaluation of Adult Chronic Chagas' Heart Disease Diagnosis by Molecular and Serological Methods

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