Evidence of Zika Virus RNA Fragments in Aedes albopictus (Diptera: Culicidae) Field-Collected Eggs From Camaçari, Bahia, Brazil

Smartt, Chelsea T.; Stenn, Tanise; Chen, Tse-Yu; Teixeira, Maria Glória; Queiroz, Erivaldo P.; Santos, Luciano Souza Dos; Queiroz, Gabriel Andrade Nonato; Souza, Katia Reis de; Silva, Luciano Kalabric; Shin, Dong Ho; Tabachnick, Walter J.

doi:10.1093/jme/tjx058

Cited by 47 publications

(36 citation statements)

References 16 publications

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“…The occurrence of Ae. albopictus arouses interest from health agencies, since, despite this vector having not been incriminated in the transmission of arboviruses in Brazil, adults and immature forms of this species have already been found naturally infected by DENV and ZIKV in Brazilian territory [66][67][68]. In addition, studies indicate the possibility of participation of Brazilian populations of this mosquito in the transmission of DENV, ZIKV, and CHIKV [69][70][71].…”

Section: Discussionmentioning

confidence: 99%

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities—Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever.

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Section: Discussionmentioning

confidence: 99%

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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“…Ae. albopictus is a significant biting pest and of public health concern (Medlock et al., ) because it is a competent vector of at least 26 arboviruses, including dengue (DENV), chikungunya (CHIKV), and Zika (ZIKV) viruses (Benedict, Levine, Hawley, & Lounibos, ; Gratz, ; Liu et al., ; Smartt et al., ; Wong, Li, Chong, Ng, & Tan, ). Its role as a primary vector of agents of recent outbreaks of both dengue fever (caused by DENV) and chikungunya fever (caused by CHIKV) (Bonizzoni, Gasperi, Chen, & James, ; Morens & Fauci, ; Paupy, Delatte, Bagny, Corbel, & Fontenille, ; Wu, Lun, James, & Chen, ) is well established.…”

Section: Introductionmentioning

confidence: 99%

“…Although its competence for the DENV and ZIKV virus does not seem to be as high as Ae. aegypti (Brady, Golding, Pigott, Kraemer, Messina, Reiner Jr, ..., Hay, ; Chouin‐Carneiro, Vega‐Rua, Vazeille, Yebakima, Girod, Goindin, ..., Failloux, ; de Lamballerie et al., ), its ability to invade and persist in temperate areas causes serious concerns for its potential role in transmission of these and other viruses (Liu et al., ; Smartt et al., ; Wong, Li, Chong, Ng, & Tan ).…”

Section: Introductionmentioning

confidence: 99%

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Population genomics of the Asian tiger mosquito, Aedes albopictus: insights into the recent worldwide invasion

Kotsakiozi

Richardson

Pichler

et al. 2017

Ecology and Evolution

111

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Aedes albopictus, the “Asian tiger mosquito,” is an aggressive biting mosquito native to Asia that has colonized all continents except Antarctica during the last ~30–40 years. The species is of great public health concern as it can transmit at least 26 arboviruses, including dengue, chikungunya, and Zika viruses. In this study, using double‐digest Restriction site‐Associated DNA (ddRAD) sequencing, we developed a panel of ~58,000 single nucleotide polymorphisms (SNPs) based on 20 worldwide Ae. albopictus populations representing both the invasive and the native range. We used this genomic‐based approach to study the genetic structure and the differentiation of Ae. albopictus populations and to understand origin(s) and dynamics of the recent invasions. Our analyses indicated the existence of two major genetically differentiated population clusters, each one including both native and invasive populations. The detection of additional genetic structure within each major cluster supports that these SNPs can detect differentiation at a global and local scale, while the similar levels of genomic diversity between native and invasive range populations support the scenario of multiple invasions or colonization by a large number of propagules. Finally, our results revealed the possible source(s) of the recent invasion in Americas, Europe, and Africa, a finding with important implications for vector‐control strategies.

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“…Despite the frequent reports of DENV serotypes and YFV vertical transmission in Aedes mosquitoes, few studies have documented this mechanism for ZIKV (Smartt et al, 2017;Costa et al, 2018) and none for MAYV.…”

mentioning

confidence: 99%

Natural vertical infection by dengue virus serotype 4, Zika virus and Mayaro virus in Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus

Maia

Bezerra

Costa

et al. 2019

Medical Vet Entomology

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Vertical transmission to progeny ensures the maintenance of arboviruses in their natural vectors. This mechanism is largely reported for dengue virus (DENV) and yellow fever virus (YFV). Few studies have addressed this mechanism for Zika virus (ZIKV), Mayaro virus (MAYV) and other arboviruses. The present study investigated the natural infection rate by arboviruses in 4490 Aedes (Stegomyia) aegypti and 296 Aedes (Stegomyia) albopictus (Diptera: Culicidae) reared from eggs collected with ovitraps in Cuiabá, Mato Grosso State, from February to July, 2017. After viral RNA extraction and reverse transcriptase‐polymerase chain reaction protocols for 10 flaviviruses and five alphaviruses, nucleotide sequencing and three passages in C6/36 cells, eight pools of Ae. aegypti positive for DENV‐4 genotype II, seven for ZIKV Asian genotype and two for MAYV genotype L were found. In addition, two Ae. albopictus pools were positive for DENV‐4 genotype II and two were positive for ZIKV Asian genotype. Infection was confirmed by viral isolation in all positive pools for DENV‐4 and for MAYV and in eight of nine for ZIKV. This mechanism may contribute to the spread of arboviruses during epidemics and also to their maintenance in natural vectors during interepidemic periods.

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Evidence of Zika Virus RNA Fragments in Aedes albopictus (Diptera: Culicidae) Field-Collected Eggs From Camaçari, Bahia, Brazil

Cited by 47 publications

References 16 publications

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Population genomics of the Asian tiger mosquito, Aedes albopictus: insights into the recent worldwide invasion

Natural vertical infection by dengue virus serotype 4, Zika virus and Mayaro virus in Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus

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