Background: Sleep disturbances affect about 40% of the global population and are a common issue among patients seeking medical consultation. There is limited research on sleep disturbances in Taiwan’s traditional industry workforce. Objective: This study aims to investigate the correlations between work patterns, health promotion behaviors, occupational burnout, and sleep disturbances among employees in traditional industries. Methods: A cross-sectional study was conducted to collect data on the work patterns, health promotion behaviors, occupational burnout, and sleep disturbances within a traditional industry. The study period was from May to June 2023. Data analysis was performed using chi-square tests, independent sample t-tests, and logistic regression. Result: Data from a total of 365 employees were collected, with 63.3% of the study subjects working in shifts and 47.9% experiencing sleep disturbances. Factors associated with sleep disturbances included personal burnout (OR = 1.07 (95% CI 1.06, 1.09); p < 0.001), shift work (OR = 2.67 (95% CI 1.53, 4.65); p < 0.001), health responsibility behavior (OR = 0.50 (95% CI 0.33, 0.77); p = 0.001), and life appreciation behavior (OR = 0.47 (95% CI 0.29, 0.76); p = 0.002). Conclusion: Occupational health nurses should regularly assess employees’ sleep status and provide psychological counseling services and health promotion programs to help employees alleviate sleep disturbances.