Evidence that acute taurine treatment alters extracellular AMP hydrolysis and adenosine deaminase activity in zebrafish brain membranes

Kist, Luiza Wilges; Etchart, Renata Jardim; Rico, Eduardo Pacheco; Langoni, Andrei Silveira; Dias, Renato Dutra; Bogo, Maurı́cio Reis; Bonan, Carla Denise; Souza, Diogo O.

doi:10.1016/j.neulet.2010.06.062

Cited by 8 publications

(5 citation statements)

References 38 publications

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“…However, lipid peroxidation is probably not the only explanation for the modulation of AChE activity, considering that the cotreatment with EtOH prevented the enzyme activation but did not reduce TBARS levels. Our recent study showed that taurine increases AMP hydrolysis and concomitantly decreases adenosine deaminase activity in zebrafish brain membranes, suggesting a potential role of adenosine in taurine-mediated effects (Rosemberg et al, 2010b). Furthermore, it is known that taurine also modulates Ca 2ϩ signaling pathways (Wu et al, 2005;Junyent et al, 2010), which are potential targets of ROS (Turrens, 2003).…”

Section: Discussionmentioning

confidence: 98%

“…Acute taurine treatments were performed using distinct concentrations (42, 150, and 400 mg•L Ϫ1 ) during 1 h, the same time used for EtOH exposure. Taurine chosen concentrations correspond to a range previously used in other studies related to taurine effects on several biochemical parameters, which vary from 0.33 to 3.2 mM (Wu et al, 2005;Kong et al, 2006;Rosemberg et al, 2010b). Two treatment protocols with taurine were performed: (i) concomitant treatment with EtOH; and (ii) a pretreatment during 1 h before EtOH exposure.…”

Section: Experimental Designmentioning

confidence: 99%

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Taurine prevents enhancement of acetylcholinesterase activity induced by acute ethanol exposure and decreases the level of markers of oxidative stress in zebrafish brain

et al. 2010

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Section: Discussionmentioning

confidence: 98%

Section: Experimental Designmentioning

confidence: 99%

Taurine prevents enhancement of acetylcholinesterase activity induced by acute ethanol exposure and decreases the level of markers of oxidative stress in zebrafish brain

et al. 2010

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“…Specifically, blockade of adenosine A 1 receptors in zebrafish attenuates the anxiogenic effect of caffeine (Maximino et al, 2011). Higher doses or longer exposure to taurine could potentially elicit an increase in brain levels of adenosine (Rosemberg et al, 2010), which may partially overcome the caffeine antagonism of adenosine receptors. Alternatively, modulation of GABA transmission may be a possible mechanism by which caffeine and taurine could regulate anxiety-like behavior.…”

Section: Discussionmentioning

confidence: 99%

Anxiety-like behavior and whole-body cortisol responses to components of energy drinks in zebrafish (Danio rerio)

Alia¹,

Petrunich-Rutherford²

2019

PeerJ

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The current study investigated the independent and combined effects of caffeine and taurine on anxiety-like behavior and neuroendocrine responses in adult zebrafish (Danio rerio). Caffeine (1,3,7-trimethylpurine-2,6-dione), the world’s most commonly used psychoactive drug, acts as an adenosine receptor blocker and a mild central nervous system stimulant. However, excessive use of caffeine is associated with heightened anxiety levels. Taurine (2-aminoethanesulfonic acid), a semi-essential amino acid synthesized within the human brain, has been hypothesized to play a role in regulating anxiolytic behavior. Caffeine and taurine are two common additives in energy drinks and are often found in high concentrations in these beverages. However, few studies have investigated the interaction of these two chemicals with regards to anxiety measures. A suitable vertebrate to examine anxiety-like behavior and physiological stress responses is the zebrafish, which has shown promise due to substantial physiological and genetic homology with humans. Anxiety-like behavior in zebrafish can be determined by analyzing habituation to novelty when fish are placed into a novel tank and scototaxis (light avoidance) behavior in the light-dark test. Stress-related neuroendocrine responses can be measured in zebrafish by analyzing whole-body cortisol levels. The goal of this study was to determine if exposure to caffeine, taurine, or a combination of the two compounds altered anxiety-like behavior and whole-body cortisol levels in zebrafish relative to control. Zebrafish were individually exposed to either caffeine (100 mg/L), taurine (400 mg/L), or both for 15 min. Zebrafish in the control group were handled in the same manner but were only exposed to system tank water. After treatment, fish were transferred to the novel tank test or the light-dark test. Behavior was tracked for the first 6 min in the novel tank and 15 min in the light-tark test. Fifteen min after introduction to the behavioral task, fish were euthanized for the analysis of whole-body cortisol levels. The results demonstrate that caffeine treatment decreased the amount of exploration in the top of the novel tank and increased scototaxis behavior in the light-dark test, which supports the established anxiogenic effect of acute exposure to caffeine. Taurine alone did not alter basal levels of anxiety-like behavioral responses nor ameliorated the anxiogenic effects of caffeine on behavior when the two compounds were administered concurrently. None of the drug treatments altered basal levels of whole-body cortisol. The current results of this study suggest that, at least at this dose and time of exposure, taurine does not mitigate the anxiety-producing effects of caffeine when administered in combination, such as with energy drink consumption.

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“…The authors demonstrated that decreasing the amount of alcohol entering the brain by taurine pretreatment could prevent the motor impairing effects of alcohol. Although it is unclear how taurine pretreatment attenuates alcohol-induced motor impairments, several studies have demonstrated that taurine inhibits the alcohol-induced increase in acetylcholinesterase activity (Rosemberg et al, 2010a) and alters extracellular adenosine monophosphate hydrolysis in the zebrafish brain (Rosemberg et al, 2010b). The behavioural changes reported here are thought to represent functional changes within the brain.…”

Section: Acute Alcohol Exposure In Zebrafishmentioning

confidence: 99%

Recent advances with a novel model organism: Alcohol tolerance and sensitization in zebrafish (Danio rerio)

Tran

2014

Progress in Neuro-Psychopharmacology and Biological Psychiatry

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Alcohol abuse and dependence is a rapidly growing problem with few treatment options available. The zebrafish has become a popular animal model for behavioural neuroscience. This species may be appropriate for investigating the effects of alcohol on the vertebrate brain. In the current review, we examine the literature by discussing how alcohol alters behaviour in zebrafish and how it may affect biological correlates. We focus on two phenomena that are often examined in the context of alcohol-induced neuroplasticity. Alcohol tolerance (a progressive decrease in the effect of alcohol over time) is often observed following continuous (chronic) exposure to low concentrations of alcohol. Alcohol sensitization also called reverse tolerance (a progressive increase in the effect of alcohol over time) is often observed following repeated discrete exposures to higher concentrations of alcohol. These two phenomena may underlie the development and maintenance of alcohol addiction. The phenotypical characterization of these responses in zebrafish may be the first important steps in establishing this species as a tool for the analysis of the molecular and neurobiological mechanisms underlying human alcohol addiction.

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Evidence that acute taurine treatment alters extracellular AMP hydrolysis and adenosine deaminase activity in zebrafish brain membranes

Cited by 8 publications

References 38 publications

Taurine prevents enhancement of acetylcholinesterase activity induced by acute ethanol exposure and decreases the level of markers of oxidative stress in zebrafish brain

Taurine prevents enhancement of acetylcholinesterase activity induced by acute ethanol exposure and decreases the level of markers of oxidative stress in zebrafish brain

Anxiety-like behavior and whole-body cortisol responses to components of energy drinks in zebrafish (Danio rerio)

Recent advances with a novel model organism: Alcohol tolerance and sensitization in zebrafish (Danio rerio)

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