Evidence that androgens and oestrogens, as well as follicle-stimulating hormone, can alter Sertoli cell number in the neonatal rat

Atanassova, Nina; Walker, Marion; McKinnell, Chris; Fisher, Jane S.; Sharpe, Richard M.

doi:10.1677/joe.1.05884

Cited by 83 publications

(55 citation statements)

References 48 publications

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“…Flutamide's reduction of testes size in the present study ( Figure 1A) is consistent with its effects in developing rats [5,17]. This reduction in testes size can be explained either by flutamide's direct antiandrogenic effect on the testes or by flutamide's up-regulation of aromatase activity and consequent increased E 2 production [16].…”

Section: Discussionsupporting

confidence: 88%

Effects of long-term flutamide treatment during development in zebra finches

Grisham

Hsia

Kim

et al. 2007

Neuroscience Letters

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The molecular mechanisms responsible for the sexual differentiation of the zebra finch song system remain mysterious. Androgen receptors are expressed in a sexually dimorphic fashion in the zebra finches song system: males have more cells expressing androgen receptors, and this sex difference appears very early in development (day 9 posthatch). Estrogen administration to hatchling females up-regulates androgen receptor expression in their song system and profoundly masculinizes their song system's morphology. Co-administering flutamide, an androgen-receptor blocker, with estrogen impedes estrogen's masculinizing effects on the song system, suggesting that androgens are required for masculine development. Accordingly, to investigate further the role of androgens in the sexual differentiation of the zebra finch song system, we sought to block androgen activity in males by administering large, sustained doses of flutamide from just before androgen receptors are expressed in the song system (day 7) through to the day of sacrifice (day 61-63).Flutamide profoundly reduced the size of the testes, demonstrating that this drug and mode of administration could have a large impact on tissues. In contrast, flutamide had only a minor impact on the song system: the number of RA neurons was slightly reduced, and the corrected HVC volume showed a trend toward demasculinization. Other brain measures (uncorrected HVC, and corrected and uncorrected volumes of Area X, lMAN, RA, and Rotundus; neuron size in lMAN, HVC, and RA; and number of HVC and LMAN neurons) were not significantly affected. The present results do not support an important role for androgen in masculinizing the song circuit after posthatch day 7.

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Section: Discussionsupporting

confidence: 88%

Effects of long-term flutamide treatment during development in zebra finches

Grisham

Hsia

Kim

et al. 2007

Neuroscience Letters

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“…It is probable also that Sertoli cells are the main target for the harmful influence of estrogen. It was found previously that DES exposure during postnatal, prepubertal period of life caused delay in advance of spermatogenesis and consequently reduced daily sperm production and testis weight in adulthood, because of delayed Sertoli cell maturation and impaired function, and decrease in their number [38,39].…”

Section: Discussionmentioning

confidence: 97%

“…Estrogen directly can retard pubertal Leydig cells development [36] and inhibit testosterone production [21,37]. Moreover, estrogen may have the direct negative influence on Sertoli cell number and maturation, independently of changes in FSH and androgen levels [38,39].…”

Section: Discussionmentioning

confidence: 99%

Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.

Filipiak

Walczak-Jędrzejowska²,

Oszukowska³

et al. 2010

Folia Histochem Cytobiol.

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Abstract:The aim of this study was to assess the impact of xenoestrogens: diethylstilbestrol (DES) and zearalenone (ZEA) on rat's pubertal testis and to compare it with the effect of natural estrogen -17β-estradiol (E). Male Wistar rats were daily, subcutaneously injected at 5 th -15 th postnatal days (p.d.) with E (1.25 or 12.5 μg) or DES (1.25 or 12.5 μg) or ZEA (4 or 40 μg) or vehicle. At 16 th p.d. testes were dissected, weighted, and paraffin embedded. Following parameters were assessed: diameter and length of seminiferous tubule, numbers of spermatogonia A+intermediate+B (A/In/B), preleptotene spermatocytes (PL), leptotene+zygotene+pachytene spermatocytes (L/Z/PA) and Sertoli cells per testis. Testes weight, seminiferous tubule diameter and length were decreased by both doses of E, DES and ZEA. DES effect was the strongest, but its influence on testis weight and seminiferous tubule length, on the contrary to E and ZEA, was not dose-dependent. Similarly, DES in both doses had the most severe negative impact on the number of germ and Sertoli cells. The negative influence of E on germ cells was less pronounced. The negative effect of ZEA was seen only after administration of the higher dose on spermatogonia number, while DES and E decreased A/In/B number more evidently. Sertoli cell number were decreased after both doses of E. ZEA40 decreased Sertoli cell number while ZEA4 had no effect. Conclusion: exposure of prepubertal male rat to DES has the strongest detrimental effect on the developing testis in comparison to E and ZEA. Both, E and DES, decreased number of germ and Sertoli cells, diminished seminiferous tubule diameter, length and testis weight. ZEA had much more weaker effect than the potent estrogens.

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“…Activin action in the reproductive system is modulated by its antagonist follistatin, which is known to be present in the rat testis at this age (Buzzard et al 2004). Activin thereby contributes to the establishment of the proper testicular size that is directly depending on Sertoli cell proliferation (Atanassova et al 2005). In turn, Sertoli cell mitotic activity is modulated by follicle-stimulating hormone (FSH)-mediated contact inhibition (Schlatt et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Initiation of testicular tubulogenesis is controlled by neurotrophic tyrosine receptor kinases in a three-dimensional Sertoli cell aggregation assay

Gassei

Ehmcke²,

Schlatt

2008

Reproduction

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The first morphological sign of testicular differentiation is the formation of testis cords. Prior to cord formation, newly specified Sertoli cells establish adhesive junctions, and condensation of somatic cells along the surface epithelium of the genital ridge occurs. Here, we show that Sertoli cell aggregation is necessary for subsequent testis cord formation, and that neurotrophic tyrosine kinase receptors (NTRKs) regulate this process. In a three-dimensional cell culture assay, immature rat Sertoli cells aggregate to form large spherical aggregates (81.36G7.34 mm in diameter) in a highly organized, hexagonal arrangement (376.95G21.93 mm average distance between spherical aggregates). Exposure to NTRK inhibitors K252a and AG879 significantly disrupted Sertoli cell aggregation in a dose-dependent manner. Sertoli cells were prevented from establishing cell-cell contacts and from forming spherical aggregates. In vitro-derived spherical aggregates were xenografted into immunodeficient nude mice to investigate their developmental potential. In controls, seminiferous tubule-like structures showing polarized single-layered Sertoli cell epithelia, basement membranes, peritubular myoid cells surrounding the tubules, and lumen were observed in histological sections. By contrast, grafts from treatment groups were devoid of tubules and only few single Sertoli cells were present in xenografts after 4 weeks. Furthermore, the grafts were significantly smaller when Sertoli cell aggregation was disrupted by K252a in vitro (20.87 vs 6.63 mg; P!0.05). We conclude from these results that NTRKregulated Sertoli-Sertoli cell contact is essential to the period of extensive growth and remodeling that occurs during testicular tubulogenesis, and our data indicate its potential function in fetal and prepubertal testis differentiation.

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Evidence that androgens and oestrogens, as well as follicle-stimulating hormone, can alter Sertoli cell number in the neonatal rat

Cited by 83 publications

References 48 publications

Effects of long-term flutamide treatment during development in zebra finches

Effects of long-term flutamide treatment during development in zebra finches

Xenoestrogens diethylstilbestrol and zearalenone negatively influence pubertal rat's testis.

Initiation of testicular tubulogenesis is controlled by neurotrophic tyrosine receptor kinases in a three-dimensional Sertoli cell aggregation assay

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