Evidence that axons containing substance P in the guinea-pig ileum are of intrinsic origin

Franco, R.; Costa, M.; Furness, John B.

doi:10.1007/bf00506552

Cited by 108 publications

(17 citation statements)

References 24 publications

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“…However, it should be emphasized that the latency of the atropine-resistant depolarization following transmural stimulation in the guinea-pig taenia coli and caecum (Shuba & Vladimirova, 1980) was much shorter than that observed in our experiments and we are therefore cautious in attributing PSD to the same transmitter as that which causes the i.j.p. Recently, Franco, Costa & Furness (1979b) have demonstrated in the guinea-pig ileum that following transmural stimulation hyoscine-resistant contractions were abolished by prior desensitization to substance P. Furthermore they have also demonstrated that a compound with substance-P-like activity is released following transmural stimulation (Franco, Costa & Furness, 1979a). Substance P or a similar compound may well be involved in the PSD responses reported in this paper.…”

Section: Effects Of Repetitive Stimulisupporting

confidence: 51%

Atropine‐resistant depolarization in the guinea‐pig small intestine

Bywater

Holman

Taylor

1981

The Journal of Physiology

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1. Junction potentials were recorded from the circular muscle cells of the guinea‐pig ileum following transmural stimulation in the presence of atropine at 30 °C. 2. Single stimuli produced a transient hyperpolarization, the inhibitory junction potential (i.j.p.). At high stimulus strengths the i.j.p. was followed by a post‐stimulus depolarization (PSD). 3. During repetitive stimulation the magnitude of the hyperpolarization decreased; however, at the end of the stimulus period the PSD was enhanced and often reached threshold for the generation of action potentials. Thus, the size of the PSD was not directly related to the degree of the preceding hyperpolarization. 4. Hyperpolarization of the circular muscle cells was produced by the application of anodal current using large external electrodes. Rapid cessation of the applied current produced a transient after‐depolarization which was shorter in time course than the PSD following the i.j.p. If the applied anodal current was reduced slowly (at a rate which mimicked the decrease in the hyperpolarization during repetitive nerve stimulation) no after‐depolarization was observed. 5. Conditioning hyperpolarization of the circular muscle cells reduced the amplitude of the i.j.p. The i.j.p. was reversed at membrane potentials greater than approximately ‐90 mV. 6. The PSD did not appear to be due to the extracellular accumulation of potassium ions following the i.j.p. since the PSD persisted even when the i.j.p. was reversed. 7. The neurotoxin apamin reversibly abolished the i.j.p. and unmasked a transient excitatory junction potential (e.j.p.) with a variable latency (350‐900 ms).

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Section: Effects Of Repetitive Stimulisupporting

confidence: 51%

Atropine‐resistant depolarization in the guinea‐pig small intestine

Bywater

Holman

Taylor

1981

The Journal of Physiology

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“…transmitters (VIP, SP and BK; Matsuzaki et al 1980;Franco, Costa & Furness, 1979a;Ferrero et at. 1980) localized in the enteric neurons (Jessen, Saffrey, Van Noorden, Bloom, Polak & Burnstock, 1980;Probert, De Mey & Polak, 1981) were also investigated in relation to the non-ch., non-adr.…”

Section: Discussionmentioning

confidence: 99%

The nature of non‐cholinergic, non‐adrenergic transmission in longitudinal and circular muscles of the guinea‐pig ileum

Bauer

Kuriyama

1982

The Journal of Physiology

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“…Immunohistochemical studies have shown substance P-like immunoreactivity exists in axons of the guineapig ileum myenteric plexus (Nilsson et al, 1975;Schultzberg et al, 1980), and substance P-like activity has been shown to be released on electrical stimulation from intrinsic neurones (Franco et al, 1979), by using specific desensitization to detect its actions. More recently, substance P release from the myenteric plexus of guinea-pig ileum longitudinal muscle preparations has been demonstrated by measuring the levels of substance P-like immunoreactivity produced on electrical stimulation (Holzer, 1983;1984;Gintzler et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

A comparison of the effects of three substance P antagonists on tachykinin‐stimulated [³H]‐acetylcholine release in the guinea‐pig ileum

Featherstone

Fosbraey

Morton

1986

British J Pharmacology

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Trp7'9,Leul ']SP( 1) and [D-Arg',D-Trp7 9'Leu' 'JSP(j Ij) (spantide) against eledoisin were examined in the guinea-pig ileum myenteric plexus, where a continuous superfusion system was employed to examine evoked release of [3H]-acetylcholine ([3H]-ACh]); effects on mechanical activity of the preparations were also measured. 2 Eledoisin was chosen as the standard tachykinin agonist since the rank order ofpotency observed in evoking release was eledoisin, kassinin, substance P, physalaemin; on this basis is may be presumed that an 'SP-E' type receptor was involved in the release process. 3 The two undecapeptide antagonists both significantly reduced the response to eledoisin (10 nM) as assessed by both [3H]-ACh release and mechanical activity which under these conditions was largely dependent on ACh release, and the response levels could be restored by increasing the concentration of eledoisin to 100 nM. The pA2 values for the two antagonists were estimated as 5. ,) being the most active in this respect, a 10 gM concentration producing 50% of the response seen with 10 nM eledoisin. 5 These findings are discussed both in relation to tachykinin receptor classifications and limitations in the use of such antagonists in the study of the role of tachykinins in neurotransmission.

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Evidence that axons containing substance P in the guinea-pig ileum are of intrinsic origin

Cited by 108 publications

References 24 publications

Atropine‐resistant depolarization in the guinea‐pig small intestine

Atropine‐resistant depolarization in the guinea‐pig small intestine

The nature of non‐cholinergic, non‐adrenergic transmission in longitudinal and circular muscles of the guinea‐pig ileum

A comparison of the effects of three substance P antagonists on tachykinin‐stimulated [³H]‐acetylcholine release in the guinea‐pig ileum

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Evidence that axons containing substance P in the guinea-pig ileum are of intrinsic origin

Cited by 108 publications

References 24 publications

Atropine‐resistant depolarization in the guinea‐pig small intestine

Atropine‐resistant depolarization in the guinea‐pig small intestine

The nature of non‐cholinergic, non‐adrenergic transmission in longitudinal and circular muscles of the guinea‐pig ileum

A comparison of the effects of three substance P antagonists on tachykinin‐stimulated [3H]‐acetylcholine release in the guinea‐pig ileum

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A comparison of the effects of three substance P antagonists on tachykinin‐stimulated [³H]‐acetylcholine release in the guinea‐pig ileum