Evidence that ERF transcriptional regulators serve as possible key molecules for natural variation in defense against herbivores in tall goldenrod

Takafuji, Kento; Rim, Hojun; Kawauchi, Kentaro; Mujiono, Kadis; Shimokawa, Saki; Ando, Yoshino; Shiojiri, Kaori; Gàlis, Ivan; Arimura, Gen Ichiro

doi:10.1038/s41598-020-62142-4

Cited by 5 publications

(3 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ERF transcription factor plays an important regulatory role in plant resistance to insect infestations [79]. For example, the combination of SSaERF1 and GCC-box can enhance the resistance of arabidopsis to Prodenia litura [80], and BrERF11b can enhance the resistance of tobacco to Myzus persicae and Prodenia litura [81], thus indicating that plant resistance to insect stress can be improved through the over-expression of ERF. BEE1, bHLH and GATA transcription factors play important role in plant resistance to biotic and abiotic stresses [82,83].…”

Section: Dmgs Involved In Regulation Of Soybean Resistance To Bean Py...mentioning

confidence: 99%

Methylome and transcriptome analyses of soybean response to bean pyralid larvae

et al. 2021

View full text Add to dashboard Cite

Background Bean pyralid is one of the major leaf-feeding insects that affect soybean crops. DNA methylation can control the networks of gene expressions, and it plays an important role in responses to biotic stress. However, at present the genome-wide DNA methylation profile of the soybean resistance to bean pyralid has not been reported so far. Results Using whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq), we analyzed the highly resistant material (Gantai-2-2, HRK) and highly susceptible material (Wan82–178, HSK), under bean pyralid larvae feeding 0 h and 48 h, to clarify the molecular mechanism of the soybean resistance and explore its insect-resistant genes. We identified 2194, 6872, 39,704 and 40,018 differentially methylated regions (DMRs), as well as 497, 1594, 9596 and 9554 differentially methylated genes (DMGs) in the HRK0/HRK48, HSK0/HSK48, HSK0/HRK0 and HSK48/HRK48 comparisons, respectively. Through the analysis of global methylation and transcription, 265 differentially expressed genes (DEGs) were negatively correlated with DMGs, there were 34, 49, 141 and 116 negatively correlated genes in the HRK0/HRK48, HSK0/HSK48, HSK0/HRK0 and HSK48/HRK48, respectively. The MapMan cluster analysis showed that 114 negatively correlated genes were clustered in 24 pathways, such as protein biosynthesis and modification; primary metabolism; secondary metabolism; cell cycle, cell structure and component; RNA biosynthesis and processing, and so on. Moreover, CRK40; CRK62; STK; MAPK9; L-type lectin-domain containing receptor kinase VIII.2; CesA; CSI1; fimbrin-1; KIN-14B; KIN-14 N; KIN-4A; cytochrome P450 81E8; BEE1; ERF; bHLH25; bHLH79; GATA26, were likely regulatory genes involved in the soybean responses to bean pyralid larvae. Finally, 5 DMRs were further validated that the genome-wide DNA data were reliable through PS-PCR and 5 DEGs were confirmed the relationship between DNA methylation and gene expression by qRT-PCR. The results showed an excellent agreement with deep sequencing. Conclusions Genome-wide DNA methylation profile of soybean response to bean pyralid was obtained for the first time. Several specific DMGs which participated in protein kinase, cell and organelle, flavonoid biosynthesis and transcription factor were further identified to be likely associated with soybean response to bean pyralid. Our data will provide better understanding of DNA methylation alteration and their potential role in soybean insect resistance.

show abstract

Section: Dmgs Involved In Regulation Of Soybean Resistance To Bean Py...mentioning

confidence: 99%

Methylome and transcriptome analyses of soybean response to bean pyralid larvae

et al. 2021

View full text Add to dashboard Cite

show abstract

“…ERF is a major subfamily of the AP2/ERF transcription factor family, and also plays an important regulatory role in plant resistance to diseases and pests [81]. For example, the combination of SSaERF1 and GCC-box can enhance the resistance of arabidopsis to Prodenia litura [82], and BrERF11b has the ability to enhance the resistance of tobacco to Myzus persicae and Prodenia litura [83], thus indicating that plant resistance to insect stress can be improved through the over-expression of ERF. In addition, bHLH are the second largest class of transcription factors in plants, and play an important role in plant growth and development, physiological metabolism, and stress response [84].…”

Section: Expression Analysis Of the Important Genes Related To Soybean Resistance To Bean Pyralid Larvaementioning

confidence: 99%

Methylome and Transcriptome Analyses of Soybean Response to Bean Pyralid Larvae

Zeng

Tan

Long

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Bean pyralid is an important leaf-feeding insect which affects soybean production. DNA methylation can control the networks of gene expressions, and it plays an important role in the growth, development, and responses to biotic stress. However, the genome-wide DNA methylation profile of the soybean resistance to bean pyralid has not been reported so far. Results: Using whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq), we analysed the highly resistant material (Gantai-2-2) and highly susceptible material (Wan82-178), under the conditions of 0 h and 48 h feeding by bean pyralid larvae, to clarify the molecular mechanism of the soybean resistance and explore its insect-resistant genes. We identified 2,194, 6,872, 39,704, and 40,018 differentially methylated regions (DMRs), as well as 497, 1,594, 9,596, and 9,554 differentially methylated genes (DMGs) in the HRK0/HRK48, HSK0/HSK48, HSK0/HRK0, and HSK48/HRK48 comparisons, respectively. We found that 265 differentially expressed genes (DEGs) were negatively correlated with the DMGs, there were 34, 49, 141, and 116 negatively correlated genes in the HRK0/HRK48, HSK0/HSK48, HSK0/HRK0, and HSK48/HRK48 comparisons, respectively. The MapMan cluster analysis results indicated that negatively correlated genes in the pathways, such as protein biosynthesis and modification; primary metabolism; secondary metabolism; cell cycle, cell structure and component; RNA biosynthesis and processing, and so on. Finally, the PS-PCR and qRT-PCR were used to validate the expression patterns of several genes and the results showed an excellent agreement with deep sequencing. Conclusions: Through the analysis of global methylation and transcription, we speculated that the expression levels of CRK40; CRK62; STK; L-type lectin-domain containing receptor kinase VIII.2; CesA; CSI1; fimbrin-1; KIN-14B; KIN-14N; KIN-4A; cytochrome P450 81E8; BEE1; ERF; SPATULA; bHLH25; bHLH79; GATA26, were regulated by methylation, and they may potentially play important roles in the soybean responses to bean pyralid larvae. Our results laid a foundation for revealing the occurrence mechanism of soybean response to bean pyralid at the level of DNA methylation.

show abstract

“…The tall goldenrod, Solidago altissima L., is an invasive alien plant with the potential to harm biodiversity on the island, although its distribution on the island is currently limited (Ryu et al 2017). S. altissima originated in North America and successfully invaded Korea, Japan, China and Europe (Nakagawa, Enomoto 1975;Takafuji et al 2020;Weber 2000). It was introduced to the Korean peninsula before 1974 for honey production (Information of Korean Alien Species, t.ly/SpgY) and is currently distributed in southwestern areas of the Korean peninsula (Kil, Kim 2014).…”

Section: Introductionmentioning

confidence: 99%

Mowing inhibits the invasion of the alien species Solidago altissima and is an effective management strategy

Rim

Lee

Kim

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Biological invasions of exotic plant species affect nutrient cycling, soil characteristics, ecosystem stability, and Biodiversity. Therefore, management measures to protect the ecosystem and native species against invasive species are becoming more important. The tall goldenrod, Solidago altissima L., is an invasive alien plant with currently limited distribution on the Jeju island, while successfully invaded areas with similar climates in China and Japan. Therefore, it has a high possibility of invasion and may have an adverse effect on the ecosystem of Jeju Island. The aim of this study was to develop an environmentally friendly management strategy to control S. altissima with understanding of the major factor that makes difference of invasion success between Japan and Jeju island. Plant communities are monitored and allelopathic effects of S. altissima are tested.. Furthermore, the effectiveness of mowing at varying frequencies and timing for the control of S. altissima were applied. S. altissima already dominates several plant communities on Jeju Island, harms the plant community via allelopathy, and reduces biodiversity. However, our study shows that mowing is an effective method to control S. altissima populations. Mowing inhibits early invasion of S. altissima and also reduces dominance and reproductive features of S. altissima where invasion has already processed. Therefore, mowing should be adopted for the management of S. altissima invasion. Mowing is an environmentally friendly management method for the control of S. altissima and could be applied to other invasive species.

show abstract

Evidence that ERF transcriptional regulators serve as possible key molecules for natural variation in defense against herbivores in tall goldenrod

Cited by 5 publications

References 49 publications

Methylome and transcriptome analyses of soybean response to bean pyralid larvae

Methylome and transcriptome analyses of soybean response to bean pyralid larvae

Methylome and Transcriptome Analyses of Soybean Response to Bean Pyralid Larvae

Mowing inhibits the invasion of the alien species Solidago altissima and is an effective management strategy

Contact Info

Product

Resources

About