Evidence that fresh weight measurement is imprecise for reporting the effect of plant growth-promoting (rhizo)bacteria on growth promotion of crop plants

Ping, Huang; de‐Bashan, Luz E.; Crocker, Trae; Kloepper, Joseph W.; Bashan, Yoav

doi:10.1007/s00374-016-1160-2

Cited by 67 publications

(34 citation statements)

References 39 publications

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“…These results were confirmed by Ahemad and Kibret [99] who reported enhanced shoot fresh and dry weights in Brassica plants inoculated with PGPR. Similar results were reported by Islam et al [100] in cucumber and by Huang et al [101] in corn, pepper and tomato. SA induced increase in shoot fresh and dry weights had been well documented in many plants including, Ocimum, lemongrass, sunflower, strawberry, wheat and maize [102].…”

Section: Plos Onesupporting

confidence: 90%

Impacts of plant growth promoters and plant growth regulators on rainfed agriculture

2020

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Demand for agricultural crop continues to escalate in response to increasing population and damage of prime cropland for cultivation. Research interest is diverted to utilize soils with marginal plant production. Moisture stress has negative impact on crop growth and productivity. The plant growth promoting rhizobacteria (PGPR) and plant growth regulators (PGR) are vital for plant developmental process under moisture stress. The current study was carried out to investigate the effect of PGPR and PGRs (Salicylic acid and Putrescine) on the physiological activities of chickpea grown in sandy soil. The bacterial isolates were characterized based on biochemical characters including Gram-staining, P-solubilisation, antibacterial and antifungal activities and catalases and oxidases activities and were also screened for the production of indole-3-acetic acid (IAA), hydrogen cyanide (HCN) and ammonia (NH 3). The bacterial strains were identified as Bacillus subtilis, Bacillus thuringiensis and Bacillus megaterium based on the results of 16S-rRNA gene sequencing. Chickpea seeds of two varieties (Punjab Noor-2009 and 93127) differing in sensitivity to drought were soaked for 3 h before sowing in fresh grown cultures of isolates. Both the PGRs were applied (150 mg/L), as foliar spray on 20 days old seedlings of chickpea. Moisture stress significantly reduced the physiological parameters but the inoculation of PGPR and PGR treatment effectively ameliorated the adverse effects of moisture stress. The result showed that chickpea plants treated with PGPR and PGR significantly enhanced the chlorophyll, protein and sugar contents. Shoot and root fresh (81%) and dry weights (77%) were also enhanced significantly in the treated plants. Leaf proline content, lipid peroxidation and antioxidant enzymes (CAT, APOX, POD and SOD) were increased in reaction to drought stress but decreased due to PGPR. The plant height (61%), grain weight (41%), number of nodules (78%) and pod (88%), plant yield (76%), pod weight (53%) and total biomass (54%) were higher in PGPR and PGR treated chickpea plants grown in sandy soil. It is concluded from the present study that the integrative use of PGPR and PGRs is a promising method and eco-friendly strategy for increasing drought tolerance in crop plants.

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Section: Plos Onesupporting

confidence: 90%

Impacts of plant growth promoters and plant growth regulators on rainfed agriculture

2020

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“…En los aislados CBCC57 y CBRF12 que tuvieron un índice de Dickson mayor al testigo, indica que estos aislados promovieron el crecimiento (Tabla 3). Por lo que los índices de calidad podrían dar una estimación más confiable para determinar el efecto de bacteria en la promoción de crecimiento, ya que el peso fresco de la planta es impreciso (Huang et al 2017). En la determinación de B-glucanasas, no se observaron diferencias significativas entre las plántulas inoculadas con Bacillus spp.…”

Section: Resultados Y Discusiónunclassified

Germinación, crecimiento y producción de glucanasas en Capsicum chinense Jacq. inoculadas con Bacillus spp.

Pech¹,

Ruíz-Sánchez²,

Suárez³

et al. 2019

Ecosist. Recur. Agropec.

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RESUMEN. Las rizobacterias promueven el crecimiento vegetal e inducen la acumulación de proteínas de defensa, por lo que el objetivo fue cuantificar la producción de ácido indolacético (AIA) en cinco aislados de Bacillus spp. Los aislados con mayor producción de AIA se usaron para evaluar la germinación, crecimiento e inducción de las B-glucanasas en plántulas de chile habanero. Los aislados CBRF5, CBRF12 y CBCC57 produjeron AIA desde las 24 h de incubación con 6.66, 7.95 y 7.05 µg mL −1 , respectivamente. Los aislados CBCC57 y CBRF5 disminuyeron el porcentaje de germinación, mientras que CBRF12 no tuvo efecto significativo. Los aislados CBCC57 y CBRF12 aumentaron el área foliar y biomasa seca en las plántulas. Se observó aumento significativo en la actividad de B-glucanasas con el aislado CBCC57 en relación a CBRF12 y CBRF5. Los resultados muestran que Bacillus CBRF12 y CBCC57 tienen potencial como promotoras del crecimiento de plántulas de chile habanero. Palabras clave: Ácido indolacético, chile habanero, índice de calidad, rizobacterias. ABSTRACT. Rhizobacteria promote plant growth and induce accumulation of defense proteins, so the objective of this study was to quantify the production of indoleacetic acid (IAA) in five isolates of Bacillus spp. The isolates with the highest IAA production were used to evaluate germination, growth and induction of B-glucanases in habanero pepper seedlings. Isolates CBRF5, CBRF12 and CBCC57 produced IAA from 24 h of incubation with 6.66, 7.95 and 7.05 µg mL-−1 , respectively. Isolates CBCC57 and CBRF5 decreased the germination percentage, while CBRF12 had no significant effect. Isolates CBCC57 and CBRF12 increased leaf area and dry biomass in the seedlings. A significant increase in B-glucanase activity was observed with isolate CBCC57 in relation to CBRF12 and CBRF5. Results show that Bacillus CBRF12 and CBCC57 have potential as growth promoters of habanero pepper seedlings.

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“…2). Similarly, though many studies have taken the increase in plant height as a marker of PGPR effect (Kang et al 2007;Muthukumar et al 2010;Xia et al 2015), Huang et al (2017) proposed that this parameter alone was not sufficient to assess the PGPR effects. In our study, the root and shoot fresh and dry weights in plants with EB applied were observed to increase by 28 to 128% (Tab.…”

Section: Peppermentioning

confidence: 99%

Effects of Endophytic Bacteria on Disease and Growth in Plants under Biotic Stress

Akköprü

Çakar

Husseini³

2018

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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The aim of this study was to reveal the effects of four endophytic bacteria (EB) (Ochrobactrum sp. CB36/1, Pantoea agglomerans CC37/2, Bacillus thuringiensis CA41/1 and Pseudomonas fluorescens CC44) on the plant development of tomato and pepper and the effects against bacterial spot disease caused by Xanthomonas euvesicatoria (Xe) in both hosts. EB applied on tomato and pepper seedlings cultivated in a sterile peat growing medium in a climate chamber in two different periods to the roots. The pathogen inoculated on the leaves by spraying. Disease severity was measured by different scales for tomatoes and peppers, and plant development parameters were determined at the end of the study. The antagonistic effects of EB against Xe and 1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase activities were determined with in vitro studies. The effect of endophytic bacteria on tomato and pepper varied according to the host plant x endophyte x pathogen combination. While no bacteria were effective against the pathogen in vitro, Ochrobactrum sp. CB36/1 inhibited the disease severity by 37% in tomato plants, but this effect was not observed in pepper. Tomato and especially pepper plants under disease stress had root and shoot fresh and dry weight increased by 28% to 128% by EB. The measurable effects of EB under biotic stress were determined to be higher than in stress-free conditions. In conclusion, the endophytic bacteria used in the study have potential for use within sustainable integrated agricultural concept framework, with their effects determined to vary according to the host, pathogen and endophytic bacteria.

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Evidence that fresh weight measurement is imprecise for reporting the effect of plant growth-promoting (rhizo)bacteria on growth promotion of crop plants

Cited by 67 publications

References 39 publications

Impacts of plant growth promoters and plant growth regulators on rainfed agriculture

Impacts of plant growth promoters and plant growth regulators on rainfed agriculture

Germinación, crecimiento y producción de glucanasas en Capsicum chinense Jacq. inoculadas con Bacillus spp.

Effects of Endophytic Bacteria on Disease and Growth in Plants under Biotic Stress

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