Evidence that locustatachykinin I is involved in release of adipokinetic hormone from locust corpora cardiaca

Nässel, Dick R.; Passier, Paul; Elekes, Károly; Dircksen, Heinrich; Vullings, H. G. B.; Cantera, Rafael

doi:10.1016/0167-0115(95)00043-b

Cited by 83 publications

(37 citation statements)

References 36 publications

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“…The findings in our study suggest, however, that knockdown of sNPF and DTK in ipc neurons produces a phenotype opposite to what would be expected if these peptides stimulate insulin signaling [58], i. e. we observe increased sensitivity to stress. We cannot totally exclude a role of sNPF and DTKs in regulation of AKH levels, since in vitro work on locust corpora cardiaca has shown that tachykinins induce release of AKH [61]. On the other hand, our finding that locomotor activity levels at starvation (presumed food search behavior) are the same in DTK knockdown and control flies suggest that DTK does not directly affect AKH levels.…”

Section: Discussionmentioning

confidence: 70%

Metabolic Stress Responses in Drosophila Are Modulated by Brain Neurosecretory Cells That Produce Multiple Neuropeptides

et al. 2010

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In Drosophila, neurosecretory cells that release peptide hormones play a prominent role in the regulation of development, growth, metabolism, and reproduction. Several types of peptidergic neurosecretory cells have been identified in the brain of Drosophila with release sites in the corpora cardiaca and anterior aorta. We show here that in adult flies the products of three neuropeptide precursors are colocalized in five pairs of large protocerebral neurosecretory cells in two clusters (designated ipc-1 and ipc-2a): Drosophila tachykinin (DTK), short neuropeptide F (sNPF) and ion transport peptide (ITP). These peptides were detected by immunocytochemistry in combination with GFP expression driven by the enhancer trap Gal4 lines c929 and Kurs-6, both of which are expressed in ipc-1 and 2a cells. This mix of colocalized peptides with seemingly unrelated functions is intriguing and prompted us to initiate analysis of the function of the ten neurosecretory cells. We investigated the role of peptide signaling from large ipc-1 and 2a cells in stress responses by monitoring the effect of starvation and desiccation in flies with levels of DTK or sNPF diminished by RNA interference. Using the Gal4-UAS system we targeted the peptide knockdown specifically to ipc-1 and 2a cells with the c929 and Kurs-6 drivers. Flies with reduced DTK or sNPF levels in these cells displayed decreased survival time at desiccation and starvation, as well as increased water loss at desiccation. Our data suggest that homeostasis during metabolic stress requires intact peptide signaling by ipc-1 and 2a neurosecretory cells.

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Section: Discussionmentioning

confidence: 70%

Metabolic Stress Responses in Drosophila Are Modulated by Brain Neurosecretory Cells That Produce Multiple Neuropeptides

et al. 2010

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“…Although effective concentrations of BRFa were much higher than BMS, this difference reflects their different modes of action on the PGs. It is well known that hormonal factors are usually effective at nanomolar concentrations, whereas peptides delivered by direct innervation act at micromolar concentrations (24)(25)(26). The even higher effective concentrations of BRFa in the heterologous expression system compared with those in PG incubated in vitro (cAMP and ecdysteroid assays) can be explained by different sensitivities of these two systems.…”

Section: Discussionmentioning

confidence: 99%

Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons

Yamanaka

Žitňan

Kim

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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In insects, steroid hormones named ecdysteroids elicit molting and metamorphosis. The prothoracic gland (PG) is a predominant source of ecdysteroids, where their biosynthesis (ecdysteroidogenesis) is regulated by several neuropeptides. Here, we report that FMRFamide-related peptides (FaRPs) regulate ecdysteroidogenesis through direct innervation of the PG in the silkworm Bombyx mori. We purified a previously uncharacterized Bombyx FaRP, DPSFIRFamide, and identified the corresponding Bombyx FMRFamide gene (Bommo-FMRFamide, BRFa), which encodes three additional FaRPs. All BRFa peptides suppressed ecdysteroidogenesis in the PG by reducing cAMP production by means of the receptor for Bommomyosuppressin, another FaRP we have previously shown to act as a prothoracicostatic factor. BRFa is predominantly expressed in neurosecretory cells of thoracic ganglia, and the neurons in the prothoracic ganglion innervate the PG to supply all four peptides to the gland surface. Electrophysiological recordings during development confirmed the increased firing activity of BRFa neurons in stages with low PG activity and decreased ecdysteroid levels in the hemolymph. To our knowledge, this study provides the first report of peptides controlling ecdysteroidogenesis by direct innervation.ecdysteroid ͉ FMRFamide-related peptides ͉ metamorphosis ͉ molting ͉ prothoracic gland

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“…Specificity tests by radioimmunoassay showed cross-reactivity of the antiserum with various C-terminally extended -MRFamides and -LRFamides (Marder et al 1987). The LomTK II antiserum (K1-50820091) was a gift from Dr. Hans Agricola; its specificity was tested by Nässel et al (1995) who showed that the serum reacted with LomTK I and II and, with lower affinity, also with callitachykinins from the fly Calliphora. As previously described, the synapsin I antibody (SYNORF1), which was kindly provided by Dr. Erich Buchner (University of Würzburg, Germany), was used selectively to label neuropilar structures including olfactory glomeruli (Berg et al 2002).…”

Section: Antiseramentioning

confidence: 99%

Distribution of neuropeptides in the primary olfactory center of the heliothine moth Heliothis virescens

et al. 2006

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Neuropeptides are a diverse widespread class of signaling substances in the nervous system. As a basis for the analysis of peptidergic neurotransmission in the insect olfactory system, we have studied the distribution of neuropeptides in the antennal lobe of the moth Heliothis virescens. Immunocytochemical experiments with antisera recognizing A-type allatostatins (AST-As), Manduca sexta allatotropin (Mas-AT), FMRFamide-related peptides (FaRPs), and tachykinin-related peptides (TKRPs) have shown that members of all four peptide families are present in local interneurons of the antennal lobe. Whereas antisera against AST-As, Mas-AT, and FaRPs give similar staining patterns characterized by dense meshworks of processes confined to the core of all antennal-lobe glomeruli, TKRPs are present only in neurons with blebby processes distributed throughout each glomerulus. In addition to local neurons, a pair of centrifugal neurons with cell bodies in the lateral subesophageal ganglion, arborizations in the antennal lobe, and projections in the inner antenno-cerebral tracts exhibits tachykinin immunostaining. Double-label immunofluorescence has detected the co-localization of AST-As, Mas-AT, and FaRPs in certain local interneurons, whereas TKRPs occurs in a distinct population. MALDI-TOF mass spectrometry has revealed nearly 50 mass peaks in the antennal lobe. Seven of these masses (four AST-As, two N-terminally extended FLRFamides, and Mas-AT) match known moth neuropeptides. The data thus show that local interneurons of the moth antennal lobe are highly differentiated with respect to their neuropeptide content. The antennal lobe therefore represents an ideal preparation for the future analysis of peptide signaling in insect brain.

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Evidence that locustatachykinin I is involved in release of adipokinetic hormone from locust corpora cardiaca

Cited by 83 publications

References 36 publications

Metabolic Stress Responses in Drosophila Are Modulated by Brain Neurosecretory Cells That Produce Multiple Neuropeptides

Metabolic Stress Responses in Drosophila Are Modulated by Brain Neurosecretory Cells That Produce Multiple Neuropeptides

Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons

Distribution of neuropeptides in the primary olfactory center of the heliothine moth Heliothis virescens

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