Evidence that retroviruses integrate into post-replication host DNA.

Hajihosseini, Mohammad K.; L, Iavachev; Price, Jack

doi:10.1002/j.1460-2075.1993.tb06190.x

Cited by 104 publications

(54 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 Such a proliferation allowed efficient retroviral-mediated transduction and selective labeling of the cell population undergoing mitosis in response to CPA. Indeed, retroviral vectors are only able to infect dividing cells, 29 and we never detected significant labeling of hepatocytes in control, untreated animals. Moreover, we observed a similar distribution of mitosis as well as hepatocyte labeling in the periportal and mediolobular zone of the lobule.…”

Section: Discussionmentioning

confidence: 53%

In vivo cell lineage analysis in cyproterone acetate-treated rat liver using genetic labeling of hepatocytes

et al. 2002

View full text Add to dashboard Cite

Genetic labeling using recombinant retroviruses is a powerful strategy for the study of cell lineage in the liver. However, this type of vector is only able to infect dividing cells. The synthetic steroid cyproterone acetate (CPA) is mitogenic and carcinogenic in the adult rat liver. In this study, we used retroviral vectors carrying the nuclear targeted ␤-galactosidase gene to selectively label and follow the fate of hepatocytes dividing on administration of CPA. Labeled cells as well as those in mitosis were preferentially located around the portal tract, whereas apoptotic bodies were predominant in the pericentral area.

show abstract

Section: Discussionmentioning

confidence: 53%

In vivo cell lineage analysis in cyproterone acetate-treated rat liver using genetic labeling of hepatocytes

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Notably, 54 Ϯ 15% of the infected cells were Olig2ϩ (n ϭ 120) and they largely consisted of NG2ϩ precursors (Fig. 3J), whereas few LM-cells were labeled, in line with the failure of MLV-based retroviral genomes to incorporate into the genome of slow proliferating cells (36). However, 1 week after virus injection, almost 50% of all GFP-labeled (GFPϩ) cells were LM- (Fig.…”

Section: Identity Of Olig2-immunopositive (Olig2؉) Cells In the Intacmentioning

confidence: 60%

Expression pattern of the transcription factor Olig2 in response to brain injuries: Implications for neuronal repair

Buffo

Voško

Ertürk

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

356

350

View full text Add to dashboard Cite

Despite the presence of neural stem cells and ongoing neurogenesis in some regions of the adult mammalian brain, neurons are not replaced in most brain regions after injury. With the aim to unravel factors contributing to the failure of neurogenesis in the injured cerebral cortex, we examined the expression of cell fate determinants after acute brain injuries, such as stab wound or focal ischemia, and in a model of chronic amyloid deposition. Although none of the neurogenic factors, such as Pax6, Mash1, Ngn2, was detected in the injured parenchyma, we observed a strong upregulation of the bHLH transcription factor Olig2, but not Olig1, upon acute and chronic injury. To examine the function of Olig2 in brain lesion, we injected retroviral vectors containing a dominant negative form of Olig2 into the lesioned cortex 2 days after a stab wound. Antagonizing Olig2 function resulted in a significant number of infected cells generating immature neurons that were not observed after injection of the control virus. These data, therefore, imply Olig2 as a repressor of neurogenesis in cells reacting to brain injury and open innovative perspectives toward evoking endogenous neuronal repair.amyloid ͉ gliosis ͉ mouse neocortex ͉ pax6 ͉ stab wound

show abstract

“…10,11 A major limitation of the currently available retroviral vectors is their requirement for target cells to transit the cell cycle in order for proviral integration to occur. 12,13 Mobilised peripheral blood stem cells are generally quiescent and are thus intrinsically refractory to transduction by such vectors. 14 Attempts to improve transduction efficiency by using cocktails of haemopoietic growth to induce entry into the cell cycle resulted in only transient marking of cells in vivo, suggesting that retroviral integration had occurred in committed progenitors rather than stem cells.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of multidrug resistance (MDR-1) gene transfer in patients undergoing high-dose therapy and peripheral blood stem cell transplantation for lymphoma

et al. 1998

View full text Add to dashboard Cite

We have performed a pilot study of MDR-1 gene transfer chain reaction (PCR) for vector-derived MDR-1 cDNA in patients receiving CD34-selected peripheral blood stem sequence in all cases. Analysis of peripheral blood and cell (PBSC) transplant for lymphoma. To ensure minimum bone marrow cells after transplant has, however, shown engraftment thresholds and facilitate CD34 purification, no evidence of in vivo gene transfer with a follow-up of 12, mobilisation of Ͼ2 × 10 6 CD34 cells/kg was a condition for 15 and 18 months. The effect of MDR-1 substrate drugs recruitment. Of 11 patients counselled for study entry, onlyhas not yet been tested as all patients remain in clinical five achieved this target in a single apheresis. In three conand radiological remission of their lymphoma. These senting patients, purified CD34 cells were exposed to results confirm the difficulty of achieving in vivo gene trans-A12M1 MDR-1 retroviral supernatant for 6 h, cryoprefer in human haemopoietic cells and indicate major logisserved then thawed and readministered following ablative tical constraints in PBSC mobilisation in patients with chemotherapy. No delay in engraftment was observed, relapsed and resistant disease in whom initial studies are although one patient received additional back-up cells.appropriate. Gene transfer was demonstrated by polymerase

show abstract

Evidence that retroviruses integrate into post-replication host DNA.

Cited by 104 publications

References 27 publications

In vivo cell lineage analysis in cyproterone acetate-treated rat liver using genetic labeling of hepatocytes

In vivo cell lineage analysis in cyproterone acetate-treated rat liver using genetic labeling of hepatocytes

Expression pattern of the transcription factor Olig2 in response to brain injuries: Implications for neuronal repair

Feasibility of multidrug resistance (MDR-1) gene transfer in patients undergoing high-dose therapy and peripheral blood stem cell transplantation for lymphoma

Contact Info

Product

Resources

About