Evidence that the large loss of glutathione observed in ischemia/reperfusion of the small intestine is not due to oxidation to glutathione disulfide

Gibson, Donald D.; Brackett, Daniel J.; Squires, Ronald A.; Balla, AndreaK.; Lerner, Megan R.; McCay, Paul B.; Pennington, Larry R.

doi:10.1016/0891-5849(93)90092-9

Cited by 36 publications

(23 citation statements)

References 33 publications

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“…The potentially harmful effects of ROS are controlled by the cellular antioxidant defense system [8]. GSH is an important constituent of intracellular protective mechanisms against a number of noxious stimuli including oxidative stress [34].…”

Section: Resultsmentioning

confidence: 99%

“…Malondialdehyde (MDA) is a secondary product of oxidative stress formed during lipid peroxidation, and it is released as a result of toxic effects of ROS [7]. Reduced glutathione (GSH), an essential component of the cellular defense mechanism against oxidative cell damage, has been used as an indicator of oxidative stress induced by ROS in the process of I/R [8]. Many experimental studies on I/R injury in animals suggest a preventive effect of antioxidants [9].…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol, a Red Wine Constituent Polyphenol, Protects from Ischemia-Reperfusion Damage of the Ovaries

Hasçalık¹,

Çelik²,

Türköz

et al. 2004

Gynecol Obstet Invest

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Objective: The aim of this study is to investigate the effects of resveratrol on histopathological changes, antioxidant status and lipid peroxidation, in torsion-detorsion injury in rat ovaries. Method: To determine whether ischemia followed by reperfusion can induce ovarian oxidative damage, we created a model of adnexal ischemia-reperfusion by using rats. Ischemia was induced by unilateral occlusion of the tubo-ovarian vessels for 3 h. Reperfusion was achieved by releasing the occlusion and restoring the circulation for 3 h. Thirty-two adult female albino rats were divided equally into 4 groups: sham operation, torsion, saline/detorsion and resveratrol/detorsion. Rats in the torsion group were killed after 360° clockwise adnexal torsion for 3 h. Resveratrol was injected intraperitoneally 30 min before detorsion in the resveratrol/detorsion group, and saline was administered in the saline/detorsion group. After 3 h of adnexal detorsion in both of these groups, the rats were killed and adnexa were removed. The tissue levels of malondialdehyde, reduced glutathione and xanthine oxidase activity were measured. Results: Malondialdehyde and xanthine oxidase levels in the saline/detorsion group were increased significantly when compared to the torsion and sham operation groups (p < 0.001). Malondialdehyde levels in the resveratrol group were lower than in the saline/detorsion group, and differences between the two groups were statistically significant (p < 0.001). Xanthine oxidase levels in the resveratrol group were lower than in the saline/detorsion and torsion groups, and differences between these groups were statistically significant (p < 0.001). Reduced glutathione levels in the saline/detorsion group were decreased significantly when compared to the torsion and sham operation groups. Reduced glutathione levels in the resveratrol group were significantly higher than in the saline/detorsion group (p < 0.006). Histological examination showed a significant improvement in ovarian morphology in the resveratrol-treated rats compared with the ischemia and ischemia-reperfusion groups. Conclusion: Our results demonstrated that intraperitoneal resveratrol administration reduced the lipid peroxidation products of ischemic rats and ovarian damage was reduced as indicated by histological examination.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Resveratrol, a Red Wine Constituent Polyphenol, Protects from Ischemia-Reperfusion Damage of the Ovaries

Hasçalık¹,

Çelik²,

Türköz

et al. 2004

Gynecol Obstet Invest

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show abstract

“…Glutathione is an important intracellular scavenger and its synthesis is dependent on the presence of glutamine. In the rat model, most of the oxidized glutathione is generated during ischemia rather than reperfusion [6]. Thus, it seems plausible to hypothesize that the presence of glutamine at the intestinal lumen may protect the gut mucosa from the deleterious effects of IRI or, at least, decelerate the evolution of the process.…”

Section: Introductionmentioning

confidence: 99%

Benefits of Intraluminal Injection of Glutamine for Intestinal Mucosa during Ischemia-Reperfusion

Aguilar-Nascimento¹,

Marques²,

Mariano³

et al. 2003

Eur Surg Res

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Background: We investigated the effect of intraluminal glutamine on the intestinal mucosa in the presence of ischemia-reperfusion injury (IRI). Methods: Six blind sacs were created in the small bowel (3 at the jejunum and 3 at the ileum) in 10 Wistar rats. The lateral sacs of both bowel regions were submitted to IRI (30/30 min), while the medial sacs were left free to receive blood supply. In the lateral sacs, a solution containing either saline plus 4% glutamine or pure saline was injected at the bowel lumen. No fluid was injected in the medial sacs. Results: Both at the jejunum and at the ileum, the score of the mucosal injury was higher in saline-injected sacs than in either glutamine-injected or control sacs. There was a significantly greater number of neutrophils in the sacs treated with saline than in the other two groups of sacs. Conclusion: Glutamine protects the mucosa and diminishes the accumulation of neutrophils at the lamina propria of the small bowel in IRI.

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“…Glutathione is an important intracellular scavenger and its synthesis is dependent on the presence of glutamine. In the rat model, most of the oxidized glutathione is generated during ischemia rather than reperfusion 7 . We have previously demonstrated that intraluminal glutamine protects the mucosal injury due to IRI in rats 8 .…”

Section: Introductionmentioning

confidence: 99%

Intraluminal injection of short chain fatty acids diminishes intestinal mucosa injury in experimental ischemia-reperfusion

et al. 2006

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PURPOSE: Investigated the effect of intraluminal short-chain fatty acids (SCFA) on the intestinal mucosa in the presence of ischemia-reperfusion injury (IRI). METHODS: Six blind sacs of the small bowel (3at the jejunum and 3 at the ileum) were created in ten Wistar rats. The lateral sacs of both bowel regions were subjected to IRI (15/15 minutes) while the medial sacs were let free to receive blood supply. In the lateral sacs, it was injected either a solution containing SCFA (butyrate, propionate and acetate) or pure saline at the bowel lumen. No fluid was injected in the medial sacs. RESULTS: Both at the jejunum and at the ileum the score of the mucosal injury was higher in saline than in control sacs. SCFA treated sacs showed lesser score at the ileum (p=0.03) but were not significantly different at the jejunum (p=0.83) when compared with saline sacs. It was found a significant greater number of neutrophils (p < 0.01) in the sacs treated with saline than in the other two sacs in both regions. CONCLUSION: SCFA protect the distal small bowel mucosa and diminishes infiltration of neutrophils to the gut lamina propria in IRI.

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Evidence that the large loss of glutathione observed in ischemia/reperfusion of the small intestine is not due to oxidation to glutathione disulfide

Cited by 36 publications

References 33 publications

Resveratrol, a Red Wine Constituent Polyphenol, Protects from Ischemia-Reperfusion Damage of the Ovaries

Resveratrol, a Red Wine Constituent Polyphenol, Protects from Ischemia-Reperfusion Damage of the Ovaries

Benefits of Intraluminal Injection of Glutamine for Intestinal Mucosa during Ischemia-Reperfusion

Intraluminal injection of short chain fatty acids diminishes intestinal mucosa injury in experimental ischemia-reperfusion

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