Evidences for an ATP-sensitive potassium channel (KATP) in muscle and fat body mitochondria of insect

Słocińska, Małgorzata; Lubawy, Jan; Jarmuszkiewicz, Wiesława; Rosiński, Grzegorz

doi:10.1016/j.jinsphys.2013.08.007

Cited by 9 publications

(4 citation statements)

References 41 publications

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“…The fact that all insects from this treatment survived, even though measured DNA damage parameters (TL, OTM, %TDNA) in the cells were high, shows that this species is equipped with a very efficient DNA repair system. Slocinska et al (2013) showed that this species possesses effective mechanisms preventing ROS formation in the muscle and fat body by regulation of the synthesis of free radicals. This energydissipating system might be implicated in cellular protection against metabolic stress in insect tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Cockroaches [Gromphadorhina coquereliana (Saussure 1863)] were reared under laboratory conditions in a continuous colony at 28°C and approximately 65% relative humidity under a 12 h:12 h light:dark cycle in the Department of Animal Physiology and Development, AMU, in Poznan. Food (lettuce, carrots and powdered milk) and water were provided ad libitum as described previously (Slocinska et al, 2013). Only adult male individuals of approximately 5.9±0.39 cm in size and a mass of 5.5±0.48 g (means±s.d.)…”

Section: Materials and Methods Insect Rearingmentioning

confidence: 99%

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Thermal stress causes DNA damage and mortality in a tropical insect

Lubawy

Daburon

Chowański

et al. 2019

Journal of Experimental Biology

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Cold tolerance is considered an important factor determining the geographic distribution of insects. We have previously shown that despite its tropical origin, the cockroach Gromphadorinha coquereliana is capable of surviving exposures to cold. However, the freezing tolerance of this species had not yet been examined. Low temperature is known to alter membrane integrity in insects, but whether chilling or freezing compromises DNA integrity remains a matter of speculation. In the present study, we subjected the G. coquereliana adults to freezing to determine their supercooling point (SCP) and evaluated whether the cockroaches were capable of surviving partial and complete freezing. Next, we conducted single cell gel electrophoresis (SCGE) assays to determine whether heat, cold and freezing altered hemocyte DNA integrity. The SCP of this species was high and around −4.76°C, which is within the typical range of freezing-tolerant species. Most cockroaches survived to 1 day after partial ice formation (20% mortality), but died progressively in the next few days after cold stress (70% mortality after 4 days). One day after complete freezing, most insects died (70% mortality), and after 4 days, 90% of them had succumbed. The SCGE assays showed substantial levels of DNA damage in hemocytes. When cockroaches were heat-stressed, the level of DNA damage was similar to that observed in the freezing treatment, though all heat-stressed insects survived. The present study shows that G. coquereliana can be considered as moderately freeze-tolerant, and that extreme low temperature stress can affect DNA integrity, suggesting that this cockroach may possess an efficient DNA repair system.

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Section: Discussionmentioning

confidence: 99%

Section: Materials and Methods Insect Rearingmentioning

confidence: 99%

Thermal stress causes DNA damage and mortality in a tropical insect

Lubawy

Daburon

Chowański

et al. 2019

Journal of Experimental Biology

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“…Prior to this study, there were knowledge gaps regarding physiological mechanisms that can be exploited to modulate ROS levels in bees, yet data presented in this study implicate K ATP channels as putative targets to regulate ROS levels and reduce virus-mediated mortality and virus replication. K ATP channels are coupled to the metabolic state of the cell that enables these channels to regulate levels of ROS in animals [ 38 ] and are used as a signaling mechanism for immune system enhancement after virus infection [ 22 , 23 , 43 , 44 , 46 ] in mammals. Exposure of bees to the K ATP channel activator pinacidil enhanced survivorship after IAPV inoculation to a degree that was not significantly different from uninfected bees at most time points (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…While the physiological and toxicological relevance of Kir channels is becoming realized within arthropods of medical and veterinary relevance [ 24 , 27 – 37 ], little is known about the role of the family of Kir channels in bees and this gap in knowledge has restricted our ability to define their fundamental roles in bee health. Interestingly, K ATP channels are coupled to the metabolic state of the cell that enables these channels to regulate levels of reactive oxygen species (ROS) through uncoupling of the mitochondria [ 38 – 42 ], which provides a protective role against hypoxic stress in Drosophila melanogaster [ 43 ] and cockroaches [ 44 ]. Further, ROS are also critical to animal survival as they can function as signal messengers for the immune system after virus infection and, thus, a moderate increase in ROS has been shown to benefit animal health by enhancing immune function [ 22 , 23 , 43 – 46 ].…”

Section: Introductionmentioning

confidence: 99%

Potassium ion channels as a molecular target to reduce virus infection and mortality of honey bee colonies

Fellows

Simone-Finstrom

Anderson

et al. 2023

Virol J

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Declines in managed honey bee populations are multifactorial but closely associated with reduced virus immunocompetence and thus, mechanisms to enhance immune function are likely to reduce viral infection rates and increase colony viability. However, gaps in knowledge regarding physiological mechanisms or ‘druggable’ target sites to enhance bee immunocompetence has prevented therapeutics development to reduce virus infection. Our data bridge this knowledge gap by identifying ATP-sensitive inward rectifier potassium (KATP) channels as a pharmacologically tractable target for reducing virus-mediated mortality and viral replication in bees, as well as increasing an aspect of colony-level immunity. Bees infected with Israeli acute paralysis virus and provided KATP channel activators had similar mortality rates as uninfected bees. Furthermore, we show that generation of reactive oxygen species (ROS) and regulation of ROS concentrations through pharmacological activation of KATP channels can stimulate antiviral responses, highlighting a functional framework for physiological regulation of the bee immune system. Next, we tested the influence of pharmacological activation of KATP channels on infection of 6 viruses at the colony level in the field. Data strongly support that KATP channels are a field-relevant target site as colonies treated with pinacidil, a KATP channel activator, had reduced titers of seven bee-relevant viruses by up to 75-fold and reduced them to levels comparable to non-inoculated colonies. Together, these data indicate a functional linkage between KATP channels, ROS, and antiviral defense mechanisms in bees and define a toxicologically relevant pathway that can be used for novel therapeutics development to enhance bee health and colony sustainability in the field.

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Guide to the Pharmacology of Mitochondrial Potassium Channels

Augustynek

Kunz

Szewczyk

2016

Handbook of Experimental Pharmacology

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This chapter provides a critical overview of the available literature on the pharmacology of mitochondrial potassium channels. In the first part, the reader is introduced to the topic, and eight known protein contributors to the potassium permeability of the inner mitochondrial membrane are presented. The main part of this chapter describes the basic characteristics of each channel type mentioned in the introduction. However, the most important and valuable information included in this chapter concerns the pharmacology of mitochondrial potassium channels. Several available channel modulators are critically evaluated and rated by suitability for research use. The last figure of this chapter shows the results of this evaluation at a glance. Thus, this chapter can be very useful for beginners in this field. It is intended to be a time- and resource-saving guide for those searching for proper modulators of mitochondrial potassium channels.

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Evidences for an ATP-sensitive potassium channel (KATP) in muscle and fat body mitochondria of insect

Cited by 9 publications

References 41 publications

Thermal stress causes DNA damage and mortality in a tropical insect

Thermal stress causes DNA damage and mortality in a tropical insect

Potassium ion channels as a molecular target to reduce virus infection and mortality of honey bee colonies

Guide to the Pharmacology of Mitochondrial Potassium Channels

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