Evidencing New Roles for the Glycosyl-Transferase Cps1 in the Phytopathogenic Fungus Botrytis cinerea

Blandenet, Matthieu; Gonçalves, Isabelle R.; Rascle, Christine; Dupuy, Jean-William; Gillet, François-Xavier; Poussereau, Nathalie; Choquer, Mathias; Bruel, Christophe

doi:10.3390/jof8090899

Cited by 6 publications

(4 citation statements)

References 63 publications

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“…In the fungal pathogen B. cinerea , the GT2 family contributes to the biogenesis and remodeling of the cell wall. This family contains the synthases for production of chitin, which plays a structural role in the fungal cell wall (Blandenet et al ., 2022). In our analysis, we observe expansion of gene families of AB hydrolases (OG0006140), glycoside hydrolase family 1 (OG0006802), and the alpha-mannosyltransferase protein (OG0007561), which may be involved in cell wall modifications in response to cold temperatures.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

Gomez,

Sic,

Haridas

et al. 2024

Preprint

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Cryomyces antarcticusis an endolithic fungus that inhabits rock outcrops in Antarctica. It survives extremes of cold, humidity and solar radiation in one of the least habitable environments on Earth. This fungus is unusual because it produces heavily melanized, meristematic growth and is thought to be haploid and asexual. Due to its growth in the most extreme environment, it has been suggested as an organism that could survive on Mars. However, the mechanisms it uses to achieve its extremophilic nature are not known. Over a billion years of fungal evolution has enabled representatives of this kingdom to populate almost all parts of planet Earth and to adapt to some of its most uninhabitable environments including extremes of temperature, salinity, pH, water, light, or other sources of radiation. Comparative genomics can provide clues to the processes underlying biological diversity, evolution, and adaptation. This effort has been greatly facilitated by the 1000 Fungal Genomes project and the JGI MycoCosm portal where sequenced genomes have been assembled into phylogenetic and ecological groups representing different projects, lifestyles, ecologies, and evolutionary histories. Comparative genomics within and between these groups provides insights into fungal adaptations, for example to extreme environmental conditions. Here, we analyze two Cryomyces genomes in the context of additional psychrophilic fungi, as well as non-psychrophilic fungi with diverse lifestyles selected from the MycoCosm database. This analysis identifies families of genes that are expanded and contracted in Cryomyces and other psychrophiles and may explain their extremophilic lifestyle. Higher GC contents of genes and of bases in the third positions of codons may help to stabilize DNA under extreme conditions. Numerous smaller contigs in C. antarcticus suggest the presence of an alternative haplotype that could indicate that the sequenced isolate is diploid or dikaryotic. These analyses provide a first step to unraveling the secrets of the extreme lifestyle ofC. antarcticus.

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Section: Discussionmentioning

confidence: 99%

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

Gomez,

Sic,

Haridas

et al. 2024

Preprint

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“…Deletion of rseA/cpsA affects hyphal growth and morphogenesis of A. nidulans and its secondary metabolite and extracellular enzyme production 15,26 . Recently, it was reported that rseA/cpsA orthologs influence plant and human pathogens 30,32,35 . Since deletion of rseA or its ortholog in N. crassa affects cell wall composition and causes hyperactivation of the CWI pathway, it has been proposed that RseA and its ortholog play a role in cell wall biogenesis 15,26,31 .…”

Section: Discussionmentioning

confidence: 99%

srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

Ogawa

Fukuda

Iwama

et al. 2023

Sci Rep

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Conidiation is an important reproductive process in Aspergillus. We previously reported, in A. nidulans, that the deletion of a putative glycosyltransferase gene, rseA/cpsA, causes an increase in the production of extracellular hydrolases and a severe reduction in conidiation. The aim of this study was to obtain novel genetic factors involved in the repression of conidiation in the rseA deletion mutant. We isolated mutants in which the rseA deletion mutant conidiation defect is suppressed and performed a comparative genomic analysis of these mutants. A gene encoding a putative transcription factor was identified as the associated candidate causative gene. The candidate gene was designated as srdA (suppressor gene for the conidiation defect of the rseAdeletion mutant). The conidiation efficiency of the rseAsrdA double-deletion mutant was increased. Introduction of wild-type srdA into the suppressor mutants caused a conidiation defect similar to that of the rseA deletion mutant. Notably, the conidiation efficiencies of the rseAsrdA double-deletion and srdA single-deletion mutants were higher than that of the wild-type strain. These results indicate that srdA is a novel genetic factor that strongly represses conidiation of the rseA deletion mutant, and a putative transcriptional regulator, SrdA is a negative regulator of conidiation in A. nidulans.

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“…Dynamic interactions between hosts and pathogens result in positive selection on proteins responsible for pathogenesis/virulence. In this study, we identified four proteins with signatures of selection (SCB64107.1, SCB65560.1, CEF85567.1, CEF79590.1), which have been shown to contribute to pathogenicity/virulence in different fungal pathogens such as: Sclerotinia sclerotiorum [59], Verticillium dahliae [60], Aspergillus flavus [61], Magnaporthe grisea [62], Botrytis cinerea [65], Candida albicans and Cryptococcus neoformans [66]. Among them, only one (SCB65560.1), to date, has been previously reported to be involved in virulence of F. graminearum [63].…”

Section: Discussionmentioning

confidence: 99%

Two distinct Fusarium graminearum populations colonized European wheat in the past two decades

Kulik,

Molcan,

Bilska

et al. 2023

PLoS ONE

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Fusarium graminearum is the main causal agent of Fusarium head blight (FHB) disease in wheat in Europe. To reveal population structure and to pinpoint genetic targets of selection we studied genomes of 96 strains of F. graminearum using population genomics. Bayesian and phylogenomic analyses indicated that the F. graminearum emergence in Europe could be linked to two independently evolving populations termed here as East European (EE) and West European (WE) population. The EE strains are primarily prevalent in Eastern Europe, but to a lesser extent also in western and southern areas. In contrast, the WE population appears to be endemic to Western Europe. Both populations evolved in response to population-specific selection forces, resulting in distinct localized adaptations that allowed them to migrate into their environmental niche. The detection of positive selection in genes with protein/zinc ion binding domains, transcription factors and in genes encoding proteins involved in transmembrane transport highlights their important role in driving evolutionary novelty that allow F. graminearum to increase adaptation to the host and/or environment. F. graminearum also maintained distinct sets of accessory genes showing population-specific conservation. Among them, genes involved in host invasion and virulence such as those encoding proteins with high homology to tannase/feruloyl esterase and genes encoding proteins with functions related to oxidation-reduction were mostly found in the WE population. Our findings shed light on genetic features related to microevolutionary divergence of F. graminearum and reveal relevant genes for further functional research aiming at better control of this pathogen.

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Evidencing New Roles for the Glycosyl-Transferase Cps1 in the Phytopathogenic Fungus Botrytis cinerea

Cited by 6 publications

References 63 publications

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

Two distinct Fusarium graminearum populations colonized European wheat in the past two decades

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