Evolución de la resistencia microbiana a fluorquinolonas en un hospital terciario

Zaragozá, J.A. Queipo; Alba, A.; garcía, E. Mascaros; Lozano, A. Gómez-Ferrer; Serrano, María Luisa Cerdeño; Cruz, Julia

doi:10.1016/s0210-4806(00)72467-3

Cited by 15 publications

(2 citation statements)

References 14 publications

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“…However, antibiotic resistance rates were higher for other enterobacteria (including the highly frequent P. mirabilis ) than for E. coli , as reported by some uroculture studies in general and pediatric populations [9, 10, 24]. P. aeruginosa has classically demonstrated high susceptibility to piperacillin-tazobactam, ceftazidime, carbapenems (mainly imipenem), and aminoglycosides, as in the present study [18, 25, 26]. Finally, although cephalosporins and aminoglycosides are slightly superior against enterobacteria in comparison to amoxicillin-clavulanic acid, Enterococcus spp.…”

Section: Discussionsupporting

confidence: 81%

Etiological and Resistance Profile of Bacteria Involved in Urinary Tract Infections in Young Children

Sorlózano-Puerto

Gómez-Luque

Luna

et al. 2017

BioMed Research International

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Background. The objective of this study was to identify the bacteria most frequently responsible for urinary tract infection (UTI) in the population of under-2-year-olds in our geographic area and to evaluate the activity of antibiotics widely used for UTI treatment during a 4-year study period. Materials and Methods. A retrospective analysis was conducted of data on the identification and susceptibility of microorganisms isolated in urine samples from children under 2 years of age. Results. A total of 1,045 uropathogens were isolated. Escherichia coli accounted for the majority (60.3%) of these, followed by Enterococcus faecalis (22.4%) and Klebsiella spp. (6.5%). The highest E. coli susceptibility rates (>90%) were to piperacillin-tazobactam, cefuroxime, cefotaxime, ceftazidime, imipenem, gentamicin, nitrofurantoin, and fosfomycin, and the lowest were to amoxicillin-clavulanic acid and cotrimoxazole. Among all bacteria isolated, we highlight the overall high activity of piperacillin-tazobactam, imipenem, nitrofurantoin, and fosfomycin against both community and hospital isolates and the reduced activity of amoxicillin-clavulanic acid, cephalosporins, gentamicin, and cotrimoxazole. There was no significant change in the total activity of any of the studied antibiotics over the 4-year study period. Conclusion. Empiric treatment with amoxicillin-clavulanic acid, cotrimoxazole, cephalosporins, and gentamicin may be inadequate due to their limited activity against uropathogens in our setting.

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Section: Discussionsupporting

confidence: 81%

Etiological and Resistance Profile of Bacteria Involved in Urinary Tract Infections in Young Children

Sorlózano-Puerto

Gómez-Luque

Luna

et al. 2017

BioMed Research International

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“…Queipo-Zaragoza et al (11) reported a significant increase in resistance to quinolones among clinical isolates of E. coli from 1990 (3%) to 1998 (23%). In 2003, resistance to ciprofloxacin in clinical isolates of E. coli obtained from blood cultures reached 19% in Spain, exceeded only by the reported rate in Portugal (9).…”

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confidence: 99%

Contribution of a New Mutation in parE to Quinolone Resistance in Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates

Sorlózano¹,

Gutiérrez²,

Jiménez

et al. 2007

J Clin Microbiol

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Mutations in the quinolone resistance-determining regions of gyrA, gyrB, parC, and parE were studied in 30 fluoroquinolone-resistant clinical isolates of Escherichia coli producing extended-spectrum ␤-lactamases. Ten isolates showed a mutation in parE that was significantly associated with an increase in the MIC for fluoroquinolones.Spain has one of the highest rates of resistance to quinolones among European countries. Queipo-Zaragoza et al. (11) reported a significant increase in resistance to quinolones among clinical isolates of E. coli from 1990 (3%) to 1998 (23%). In 2003, resistance to ciprofloxacin in clinical isolates of E. coli obtained from blood cultures reached 19% in Spain, exceeded only by the reported rate in Portugal (9). However, these data are very likely biased (1).There has also been an increase in the use of fluoroquinolones, especially cephalosporins, to treat enterobacterial infections, which has in turn increased the appearance of extendedspectrum ␤-lactamases (ESBLs) among these infections. The association between ESBL production and fluoroquinolone resistance is well documented (8) and may be due in part to the joint transfer of both mechanisms via plasmids (10). Nevertheless, this explains only a minimal proportion of the coresistance found.The objective of this study was to investigate the presence of mutations in regions that code for quinolone resistance in the chromosomal genes gyrA, gyrB, parC, and parE in ESBL-producing clinical isolates of Escherichia coli.Thirty previously characterized (13-15) ESBL-producing clinical isolates of E. coli were characterized for fluoroquinolone resistance using a microdilution assay for ciprofloxacin and levofloxacin (2). Reference strains Klebsiella pneumoniae ATCC 700603 and E. coli ATCC 25922 served as controls in all MIC determinations. PCR and direct DNA sequencing were used to identify mutations. The oligonucleotide primers 5Ј-ACGTACTAGGCAATGACTGG-3Ј and 5Ј-AGAAGTCGC CGTCGATAGAAC-3Ј were used to amplify nucleotides of gyrA, generating a fragment of 189 bp; 5Ј-TGTATGCGATGTC TGAACTG-3Ј and 5Ј-CTCAATAGCAGCTCGGAATA-3Ј were used for parC (264-bp fragment); and 5Ј-TACCGAG CTGTTCCTTGTGG-3Ј and 5Ј-GGCAATGTGCAGACCAT CAG-3Ј were used for parE (266-bp fragment), following a previously described procedure (4). Finally, the oligonucleotide primers 5Ј-CTCCTCCCAGACCAAAGACA-3Ј and 5Ј-TCACGACCGATACCACAGCC-3Ј were used for gyrB (447-bp fragment), following a previously described procedure (17). Amplified DNA products were resolved by electrophoresis in agarose (1.6%, wt/vol) gels containing ethidium bromide. The PCR product was purified (Wizard PCR Preps DNA purification system; Promega, Madison, WI), processed with a DNA sequencing kit, and analyzed in an automatic DNA se-

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Infección urinaria en pacientes con vejiga neurógena: patrones de resistencias de los uropatógenos más frecuentes

Romero-Cullerés

Planells-Romeo

Salazar-Muñoz

et al. 2012

Actas Urológicas Españolas

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Evolución de la resistencia microbiana a fluorquinolonas en un hospital terciario

Cited by 15 publications

References 14 publications

Etiological and Resistance Profile of Bacteria Involved in Urinary Tract Infections in Young Children

Etiological and Resistance Profile of Bacteria Involved in Urinary Tract Infections in Young Children

Contribution of a New Mutation in parE to Quinolone Resistance in Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates

Infección urinaria en pacientes con vejiga neurógena: patrones de resistencias de los uropatógenos más frecuentes

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