Evolution and applications of Next Generation Sequencing and its intricate relations with chromatographic and spectrometric techniques in modern day sciences

Katara, Annapurna; Chand, Sumit; Chaudhary, Harshvardhan; Chaudhry, Vijeta; Chandra, Harish; Dubey, Ramesh Chandra

doi:10.1016/j.jcoa.2024.100121

Cited by 3 publications

(1 citation statement)

References 162 publications

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“…A few decades ago, scientists studied single genes at a time. Current technological innovations allow fast and cost-effective sequencing of entire genomes [ 17 ]. This tremendous change in sequencing capability is reflected in the first human genome, which was produced by an international consortium that cost billions of dollars; today, the sequencing of individual genomes can be completed for a reasonable price in a relatively short time [ 18 ].…”

Section: Genomics and Transcriptomicsmentioning

confidence: 99%

Multi Omics Applications in Biological Systems

Gutierrez Reyes,

Alejo-Jacuinde,

Perez Sanchez

et al. 2024

CIMB

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Traditional methodologies often fall short in addressing the complexity of biological systems. In this regard, system biology omics have brought invaluable tools for conducting comprehensive analysis. Current sequencing capabilities have revolutionized genetics and genomics studies, as well as the characterization of transcriptional profiling and dynamics of several species and sample types. Biological systems experience complex biochemical processes involving thousands of molecules. These processes occur at different levels that can be studied using mass spectrometry-based (MS-based) analysis, enabling high-throughput proteomics, glycoproteomics, glycomics, metabolomics, and lipidomics analysis. Here, we present the most up-to-date techniques utilized in the completion of omics analysis. Additionally, we include some interesting examples of the applicability of multi omics to a variety of biological systems.

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Section: Genomics and Transcriptomicsmentioning

confidence: 99%

Multi Omics Applications in Biological Systems

Gutierrez Reyes,

Alejo-Jacuinde,

Perez Sanchez

et al. 2024

CIMB

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The Prospect of Gene Exploitation through Soil Metagenomics

Sagar,

Priti,

Gupta

et al. 2024

J. Pure Appl. Microbiol.

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The quantity and diversity of the microbial community in soil make it possibly the most difficult of all the natural ecosystems. It is thought to be challenging to culture up to 99% of the microorganisms in a given environment. The intricacy of microbial variety is impacted by numerous interconnected factors, including as soil structure, water content, biotic activity, pH, and fluctuations in climate. Environmental DNA isolation and purification are often the first steps in the soil metagenomic analysis process. Creating genomic DNA libraries and then using them for high-throughput sequencing or library screening are the main steps in the application of metagenomics. These genomic sequences are currently being used to advance our knowledge of the ecology and physiology of these bacteria as well as for new biotechnological and medicinal applications. To completely comprehend the intricacies involved in the operation of microbial communities and the interactions between different microorganisms within specific niches, metagenomic sequences are employed. This study focuses on the latest advancements in biotechnological approaches and procedures for identifying novel genes from uncultured microorganisms and intricate microbial habitats.

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Exploring plant-microbe interactions in adapting to abiotic stress under climate change: a review

Muhammad,

Kong,

Zheng

et al. 2024

Front. Plant Sci.

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Climatic change and extreme weather events have become a major threat to global agricultural productivity. Plants coexist with microorganisms, which play a significant role in influencing their growth and functional traits. The rhizosphere serves as an ecological niche encompassing plant roots and is a chemically complex environment that supports the growth and development of diverse plant-interactive microbes. Although plant-microbe interactions have been extensively investigated however, limited exploration have been made how abiotic stresses affect the structure and assembly of microbial communities in the rhizosphere. This review highlights climate change influence on plant growth, functional traits, and microbial communities. It explores plant mechanisms for mitigating abiotic stress, such as removing reactive oxygen species (ROS), regulating antioxidant activity and indole-3-acetic acid (IAA) production, and controlling growth-inhibitory ethylene levels through colonization by bacteria producing ACC deaminase. Additionally, we elaborated the systematic communicatory network steered by hormonal crosstalk and root exudation, which can modulate and initiate the dialogues between plants and surrounding microbes. This network ultimately promotes the chemotactic movement of microbes towards the rhizosphere, facilitating their early colonization. Finally, we reviewed the recent advancements for understanding how plant-microbe interactions foster resilience under climate stress.

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Evolution and applications of Next Generation Sequencing and its intricate relations with chromatographic and spectrometric techniques in modern day sciences

Cited by 3 publications

References 162 publications

Multi Omics Applications in Biological Systems

Multi Omics Applications in Biological Systems

The Prospect of Gene Exploitation through Soil Metagenomics

Exploring plant-microbe interactions in adapting to abiotic stress under climate change: a review

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