Evolution and involution of atherosclerosis and its relationship with vascular reactivity in hypercholesterolemic rabbits

Ozaki, Michiko Regina; Almeida, Eros Antônio de

doi:10.1016/j.etp.2011.09.006

Cited by 11 publications

(13 citation statements)

References 22 publications

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“…However, this effect occurred only with doses ≥ 0.5 mg, meaning that lower doses are unable to exercise the same effect during the time period of the experiment. Similar results in endothelial dysfunction reversal by pitavastatin have been reported in other experimental studies 19 and in humans 29 without great differences from those observed when other statins were assessed 28,30-32 .…”

Section: Discussionsupporting

confidence: 89%

“…Closely related to both situations, an improvement in endothelial dysfunction and decreased lipid oxidation is the reduction in tissue cholesterol, as reported in Table 1 and Figure 5, as well as in previous studies 28,30-32 . However the reduction in tissue cholesterol and lipid peroxidation occur similarly in all treated groups, unlike endothelial dysfunction reversal, which was observed only in the groups where animals received higher doses of pitavastatin (G5 and G6).…”

Section: Discussionsupporting

confidence: 81%

“…In another experimental study in ovariectomized hypercholesterolemic rabbits 19 , the authors found no significant changes in HDL and triglycerides. These findings regarding HDL have been observed with other statins in the literature 30,32 , and they were not aimed at specifying the mechanisms by which these animals exhibit such behavior. Differences in lipid metabolism between species that justify such results should exist and should be the objectives of future researches in order to better understand this phenomenon.…”

Section: Discussionmentioning

confidence: 79%

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Effect of Pitavastatin on Vascular Reactivity in Hypercholesterolemic Rabbits

Almeida¹,

Ozaki²

2014

Arquivos Brasileiros De Cardiologia

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BackgroundPitavastatin is the newest statin available in Brazil and likely the one with fewer side effects. Thus, pitavastatin was evaluated in hypercholesterolemic rabbits in relation to its action on vascular reactivity.ObjectiveTo assess the lowest dose of pitavastatin necessary to reduce plasma lipids, cholesterol and tissue lipid peroxidation, as well as endothelial function in hypercholesterolemic rabbits.MethodsThirty rabbits divided into six groups (n = 5): G1 - standard chow diet; G2 - hypercholesterolemic diet for 30 days; G3 - hypercholesterolemic diet and after the 16th day, diet supplemented with pitavastatin (0.1 mg); G4 - hypercholesterolemic diet supplemented with pitavastatin (0.25 mg); G5 - hypercholesterolemic diet supplemented with pitavastatin (0.5 mg); G6 - hypercholesterolemic diet supplemented with pitavastatin (1.0 mg). After 30 days, total cholesterol, HDL, triglycerides, glucose, creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT) were measured and LDL was calculated. In-depth anesthesia was performed with sodium thiopental and aortic segments were removed to study endothelial function, cholesterol and tissue lipid peroxidation. The significance level for statistical tests was 5%.ResultsTotal cholesterol and LDL were significantly elevated in relation to G1. HDL was significantly reduced in G4, G5 and G6 when compared to G2. Triglycerides, CK, AST, ALT, cholesterol and tissue lipid peroxidation showed no statistical difference between G2 and G3-G6. Significantly endothelial dysfunction reversion was observed in G5 and G6 when compared to G2.ConclusionPitavastatin starting at a 0.5 mg dose was effective in reverting endothelial dysfunction in hypercholesterolemic rabbits.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 79%

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Effect of Pitavastatin on Vascular Reactivity in Hypercholesterolemic Rabbits

Almeida¹,

Ozaki²

2014

Arquivos Brasileiros De Cardiologia

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“…The HFD was rich in fat, but with relatively less protein and fiber. In this study, we did not utilize Watanabe heritable hyperlipidemic rabbits according to Ozaki and de Almeida16, who fed healthy male New Zealand rabbits a hypercholesterolemic diet.…”

Section: Discussionmentioning

confidence: 99%

The effects of high-fat diet on implant osseointegration: an experimental study

Dündar

Yaman

Ozupek

et al. 2016

J Korean Assoc Oral Maxillofac Surg

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ObjectivesIn this study, we investigated whether a high-fat diet (HFD) affected the bone implant connection (BIC) in peri-implant bone.Materials and MethodsFour male rabbits were used in this study. Dental implant surgery was introduced into each tibia, and four implants were integrated into each animal. In both the normal diet (ND) group (n=2) and HFD group (n=2), 8 implants were integrated, for a total of 16 integrated implants. The animals continued with their respective diets for 12 weeks post-surgery. Afterward, the rabbits were sacrificed, and the BIC was assessed histomorphometrically.ResultsHistologic and histomorphometric analyses demonstrated that BIC was not impaired in the HFD group compared to the ND group.ConclusionWithin the limitations of this study, we found that HFD did not decrease the BIC in rabbit tibias.

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“…In human coronary atherosclerotic plaques, a very large proportion of foam cells are SMC-derived [7]. However, the mechanisms by which SMC contribute to plaque formation are not fully elucidated [9,10]. …”

Section: Introductionmentioning

confidence: 99%

Pro-atherogenic role of smooth muscle Nox4-based NADPH oxidase

Tong

Khandelwal

et al. 2016

Journal of Molecular and Cellular Cardiology

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Nox4-based NADPH oxidase is a major reactive oxygen species-generating enzyme in the vasculature, but its role in atherosclerosis remains controversial. Objective Our goal was to investigate the role of smooth muscle Nox4 in atherosclerosis. Approach and results Atherosclerosis-prone conditions (disturbed blood flow and Western diet) increased Nox4 mRNA level in smooth muscle of arteries. To address whether upregulated smooth muscle Nox4 under atherosclerosis-prone conditions was directly involved in the development of atherosclerosis, mice carrying a human Nox4 P437H dominant negative mutation (Nox4DN), specifically in smooth muscle, were generated on a FVB/N ApoE deficient genetic background to counter the effect of increased smooth muscle Nox4. Nox4DN significantly decreased aortic stiffness and atherosclerotic lesions, with no effect on blood pressure. Gene analysis indicated that soluble epoxide hydrolase 2 (sEH) was significantly downregulated in Nox4DN smooth muscle cells (SMC), at both mRNA and protein levels. Downregulation of sEH by siRNA decreased SMC proliferation and migration, and suppressed inflammation and macrophage adhesion to SMC. Conclusions Downregulation of smooth muscle Nox4 inhibits atherosclerosis by suppressing sEH, which, at least in part, accounts for inhibition of SMC proliferation, migration and inflammation.

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Evolution and involution of atherosclerosis and its relationship with vascular reactivity in hypercholesterolemic rabbits

Cited by 11 publications

References 22 publications

Effect of Pitavastatin on Vascular Reactivity in Hypercholesterolemic Rabbits

Effect of Pitavastatin on Vascular Reactivity in Hypercholesterolemic Rabbits

The effects of high-fat diet on implant osseointegration: an experimental study

Pro-atherogenic role of smooth muscle Nox4-based NADPH oxidase

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