Evolution and Systematics ofAnopheles:Insights from a Molecular Phylogeny of Australasian Mosquitoes

Foley, Desmond H.; Bryan, Joan H.; Yeates, David K.; Saul, Allan

doi:10.1006/mpev.1997.0457

Cited by 109 publications

(129 citation statements)

References 32 publications

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“…farauti population samples and its inferred sister species, An. irenicus (Foley et al 1998;Beebe et al 2000a). The well-supported clades in the consensus tree clearly demonstrate species-specific divergence of the ITS1 at the nucleotide level that is concordant with the previously estimated relationships for these species (Fig.…”

Section: Internal Repeats and Secondary Structuresupporting

confidence: 88%

“…farauti and An. irenicus had previously been inferred in two other studies (Foley et al 1998;Beebe et al 2000a) and was also shown by the ITS1. There was high bootstrap support for the grouping of each clone with its species and population source, which clearly indicates the occurrence of concerted evolution at the nucleotide level.…”

Section: Its1 Internal Repeatssupporting

confidence: 70%

“…Length variation was examined using cloned PCR isolates and Southern blot hybridization. The species relationships are well supported by two concordant phylogenetic hypotheses, using the mitochondrial COII gene (Foley et al 1998) and 18S rDNA (Beebe et al 2000a). This enabled us to use the phylogeny to interpret the molecular evolution of the internal repeats.…”

Section: Introductionmentioning

confidence: 76%

“…farauti complex (Foley et al 1998;Beebe et al 2000a). The DNA substitutional model chosen by MODELTEST (Posada and Crandall 1998) was the general time-reversible (GTR) model (Rodríguez et al 1990) with a proportion of sites treated as potentially variable that are drawn from a gamma distribution (U) and the remainder Fig.…”

Section: Internal Repeats and Secondary Structurementioning

confidence: 99%

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Internal Repetition and Intraindividual Variation in the rDNA ITS1 of the Anopheles punctulatus Group (Diptera: Culicidae): Multiple Units and Rates of Turnover

Bower

Cooper²,

Beebe

2009

J Mol Evol

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The rapid divergence of repetitive sequences makes them desirable markers for phylogenetic studies of closely related groups, provided that a high level of sequence homogeneity has been maintained within species. Intraspecific polymorphisms are found in an increasing number of studies now, and this highlights the need to determine why these occur. In this study we examined intraindividual variation present in the first ribosomal internal transcribed spacer (ITS1) from a group of cryptic mosquito species. Individuals of the Anopheles punctulatus group contained multiple ITS1 length variants that ranged from 1.2 to 8.0 kb. Nucleotide and copy number variation for several homologous internal repeats is common, yet the intraspecific sequence divergence of cloned PCR isolates is comparable to that of other mosquito species (*0.2-1.5%). Most of the length variation is comprised of a 5 0 -ITS1 repeat that was identified as a duplication of a conserved ITS2 region. Secondary structure conservation for this repeat is pronounced and several repeat types that are highly homogenized have formed. Significant interspecific divergence indicates a high rate of evolutionary change for this spacer. A maximum likelihood tree constructed here was congruent with previous phylogenetic hypotheses and suggests that concerted evolution is also accompanied by interpopulation divergence. The lack of interindividual differences and the presence of homogenized internal repeats suggest that a high rate of turnover has reduced the overall level of variation. However, the intraindividual variation also appears to be maintained by the absence of a single turnover rate and the complex dynamics of ongoing recombination within the spacer.

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Section: Internal Repeats and Secondary Structuresupporting

confidence: 88%

Section: Its1 Internal Repeatssupporting

confidence: 70%

Section: Introductionmentioning

confidence: 76%

Section: Internal Repeats and Secondary Structurementioning

confidence: 99%

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Internal Repetition and Intraindividual Variation in the rDNA ITS1 of the Anopheles punctulatus Group (Diptera: Culicidae): Multiple Units and Rates of Turnover

Bower

Cooper²,

Beebe

2009

J Mol Evol

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“…Based on mtDNA, this taxon is distinct from both A. sundaicus s.s. and A. epiroticus. Phylogenetic differentiation related to equivalent genetic divergence, such as those observed between members of Anopheles species complexes (Foley et al, 1998), suggests the existence of possible new species (Cracraft, 1983).…”

Section: Taxonomic Statusmentioning

confidence: 99%

Speciation and phylogeography of the Southeast Asian Anopheles sundaicus complex

Dusfour

Michaux

Harbach

et al. 2007

Infection, Genetics and Evolution

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Anopheles sundaicus s.l. is a malaria vector in coastal areas of Southeast Asia. Previous studies showed at least four distinct species within the complex. The present study investigated the phylogeography and the status of A. sundaicus s.l. populations from Cambodia, Thailand, Malaysia and Indonesia with regard to A. sundaicus s.s. from Sarawak, Malaysian Borneo and A. epiroticus in Vietnam and Thailand. Three lineages recovered by analyses of Cyt-b and COI (mtDNA) confirmed the presence of A. sundaicus s.s. in Malaysian Borneo, the distribution of A. epiroticus from southern Vietnam to peninsular Malaysia, and recognised a distinct form in Indonesia that is named A. sundaicus E. The phylogenetic and demographic analyses suggest that the three species were separated during the Early Pleistocene (1.8-0.78 Myr) and experienced bottlenecks followed by a genetic expansion in more recent times. Based on the results and knowledge of the biogeography of the area, we hypothesise that the combination of cyclical island and refugium creation was the cause of lineage isolation and bottleneck events during the Pleistocene. #

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Molecular identification of mosquitoes (Diptera: Culicidae) in southeastern Australia

Batovska

Blacket

Brown

et al. 2016

Ecology and Evolution

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DNA barcoding is a modern species identification technique that can be used to distinguish morphologically similar species, and is particularly useful when using small amounts of starting material from partial specimens or from immature stages. In order to use DNA barcoding in a surveillance program, a database containing mosquito barcode sequences is required. This study obtained Cytochrome Oxidase I (COI) sequences for 113 morphologically identified specimens, representing 29 species, six tribes and 12 genera; 17 of these species have not been previously barcoded. Three of the 29 species ─ Culex palpalis, Macleaya macmillani, and an unknown species originally identified as Tripteroides atripes ─ were initially misidentified as they are difficult to separate morphologically, highlighting the utility of DNA barcoding. While most species grouped separately (reciprocally monophyletic), the Cx. pipiens subgroup could not be genetically separated using COI. The average conspecific and congeneric p‐distance was 0.8% and 7.6%, respectively. In our study, we also demonstrate the utility of DNA barcoding in distinguishing exotics from endemic mosquitoes by identifying a single intercepted Stegomyia aegypti egg at an international airport. The use of DNA barcoding dramatically reduced the identification time required compared with rearing specimens through to adults, thereby demonstrating the value of this technique in biosecurity surveillance. The DNA barcodes produced by this study have been uploaded to the ‘Mosquitoes of Australia–Victoria’ project on the Barcode of Life Database (BOLD), which will serve as a resource for the Victorian Arbovirus Disease Control Program and other national and international mosquito surveillance programs.

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Evolution and Systematics ofAnopheles:Insights from a Molecular Phylogeny of Australasian Mosquitoes

Cited by 109 publications

References 32 publications

Internal Repetition and Intraindividual Variation in the rDNA ITS1 of the Anopheles punctulatus Group (Diptera: Culicidae): Multiple Units and Rates of Turnover

Internal Repetition and Intraindividual Variation in the rDNA ITS1 of the Anopheles punctulatus Group (Diptera: Culicidae): Multiple Units and Rates of Turnover

Speciation and phylogeography of the Southeast Asian Anopheles sundaicus complex

Molecular identification of mosquitoes (Diptera: Culicidae) in southeastern Australia

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