The construction of mountain tunnels can lead to groundwater loss and severely impact plant growth. In order to study the limited discharge of groundwater in mountain tunnels for the normal growth of typical herbaceous plants, a tunnel in the alpine meadow area of Qinghai Province was taken as the research objective. Based on transplant experiments, numerical simulations, and the empirical calculation of tunnel discharge limits, the minimum water level required for the normal growth of herbaceous plants, groundwater changes, and grouting parameters during tunnel construction, as well as limited discharge values of groundwater based on the normal growth requirements of plants, were studied. The results indicate that when the groundwater level declined by 0.6–0.8 m, herbaceous plants were able to normally grow. Generally, tunnel excavation lowered the groundwater level so that the normal growth of herbaceous plants was significantly affected. The reasonable grouting parameters were obtained by numerical simulation. They were able to ensure that the groundwater level decline was less than 0.8 m and ultimately recovered to over 90% of the pre-construction level. The herbaceous plants in Qinghai’s alpine grasslands were able to normally grow when the groundwater discharge limit was 0.2~4.0 m3/(m·d). This research offers guidance and support for managing groundwater discharge during tunnel construction in ecologically fragile areas, such as the Three Rivers Source in Qinghai.