Evolution of a chordate-specific mechanism for myoblast fusion

Zhang, Haifeng; Shang, Renjie; Kim, Kwantae; Zheng, Wei; Johnson, Christopher; Sun, Lei; Niu, Xiang; Liu, Liang; Zhou, Jingqi; Liu, Lingshu; Zhang, Zheng; Uyeno, Theodore A.; Pei, Jimin; Fissette, Skye D.; Green, Stephen A.; Samudra, Sukhada P.; Wen, Junfei; Zhang, Jianli; Eggenschwiler, Jonathan; Menke, Douglas B.; Bronner‐Fraser, Marianne; Grishin, Nick V.; Li, Weiming; Ye, Kaixiong; Zhang, Yang; Stolfi, Alberto; Bi, Pengpeng

doi:10.1126/sciadv.add2696

Cited by 13 publications

(13 citation statements)

References 82 publications

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“…Myoblasts are undifferentiated cells implicated in the repair of muscle injury and skeletal muscle development during embryonic growth (Zhang et al, 2022). During myoblasts differentiation, the ER is prone to dysregulation of homeostasis, inducing ER stress and culminating in the unfolded protein response (UPR), an evolutionarily conserved mechanism of cell survival (Nakanishi et al, 2007).…”

Section: Biological Functionsmentioning

confidence: 99%

Glycogen‐binding protein STBD1: Molecule and role in pathophysiology

Tang

Liu

Zhao

et al. 2023

Journal Cellular Physiology

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Starch‐binding domain‐containing protein 1 (STBD1) is a glycogen‐binding protein discovered in skeletal muscle gene differential expression that is pivotal to cellular energy metabolism. Recent studies have indicated that STBD1 is involved in many physiological processes, such as glycophagy, glycogen accumulation, and lipid droplet formation. Moreover, dysregulation of STBD1 causes multiple diseases, including cardiovascular disease, metabolic disease, and even cancer. Deletions and/or mutations in STBD1 promote tumorigenesis. Therefore, STBD1 has garnered considerable interest in the pathology community. In this review, we first summarized the current understanding of STBD1, including its structure, subcellular localization, tissue distribution, and biological functions. Next, we examined the roles and molecular mechanisms of STBD1 in related diseases. Based on available research, we discussed the novel function and future of STBD1, including its potential application as a therapeutic target in glycogen‐related diseases. Given the significance of STBD1 in energy metabolism, an in‐depth understanding of the protein is crucial for understanding physiological processes and developing therapeutic strategies for related diseases.

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Section: Biological Functionsmentioning

confidence: 99%

Glycogen‐binding protein STBD1: Molecule and role in pathophysiology

Tang

Liu

Zhao

et al. 2023

Journal Cellular Physiology

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“…Although we have used ASEC-1 to explore Hh signaling here, this cell line can be used to broadly explore gene function in anoles. We note that Zhang and colleagues were able to isolate a subclone of ASEC-1 that can be induced to initiate myogenesis in culture (Zhang et al, 2022). They subsequently used this subclone to investigate the function of the mymk gene in lizard myoblasts.…”

Section: Understanding the Role Of Primary Cilia In Reptile Hh Signal...mentioning

confidence: 99%

A new cell culture resource for investigations of reptilian gene function

Samudra,

Park,

Esser

et al. 2023

Preprint

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The recent establishment of CRISPR/Cas9 gene editing inA. sagreilizards makes it a powerful model system for studies of reptilian gene function. To enhance the versatility of this model, we developed an immortalized lizard fibroblast cell line (ASEC-1) for the exploration of reptilian gene function in cellular processes. We demonstrate the use of thisin vitrosystem by scrutinizing the role of primary cilia in lizard Hedgehog (Hh) signaling. Through CRISPR/Cas9 mutagenesis we disrupted theift88gene, which is required for ciliogenesis in diverse organisms. We find that the loss ofitf88from lizard cells results in an absence of primary cilia, a partial derepression ofgli1transcription, and an inability of the cells to respond to the Smoothened agonist, SAG. Through a cross-species analysis of SAG-induced transcriptional responses in cultured limb bud cells, we further determined that ∼46% of genes induced as a response to Hh pathway activation inA. sagrei,are also SAG-responsive inM. musculuslimb bud cells. Our results highlight conserved and diverged aspects of Hh signaling in anoles and establish a new resource for investigations of reptilian gene function.

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“…A recent study has suggested that MYMK and MYMX have coevolved in the vertebrates [119]. These observations indicate that MYMK and MYMX evolved together for myoblast fusion, parallel to the emergence of sarcomeric muscle and EC coupling in this lineage [120].…”

Section: Divergent Fusion Machinery Between Amniotes and Anamniotesmentioning

confidence: 99%

Ca²⁺ as a coordinator of skeletal muscle differentiation, fusion and contraction

2022

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Muscle regeneration is essential for vertebrate muscle homeostasis and recovery after injury. During regeneration, muscle stem cells differentiate into myocytes, which then fuse with pre-existing muscle fibres. Hence, differentiation, fusion and contraction must be tightly regulated during regeneration to avoid the disastrous consequences of premature fusion of myocytes to actively contracting fibres. Cytosolic calcium (Ca 2+ ), which is coupled to both induction of myogenic differentiation and contraction, has more recently been implicated in the regulation of myocyte-to-myotube fusion. In this viewpoint, we propose that Ca 2+ -mediated coordination of differentiation, fusion and contraction is a feature selected in the amniotes to facilitate muscle regeneration.

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Evolution of a chordate-specific mechanism for myoblast fusion

Cited by 13 publications

References 82 publications

Glycogen‐binding protein STBD1: Molecule and role in pathophysiology

Glycogen‐binding protein STBD1: Molecule and role in pathophysiology

A new cell culture resource for investigations of reptilian gene function

Ca²⁺ as a coordinator of skeletal muscle differentiation, fusion and contraction

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Evolution of a chordate-specific mechanism for myoblast fusion

Cited by 13 publications

References 82 publications

Glycogen‐binding protein STBD1: Molecule and role in pathophysiology

Glycogen‐binding protein STBD1: Molecule and role in pathophysiology

A new cell culture resource for investigations of reptilian gene function

Ca2+ as a coordinator of skeletal muscle differentiation, fusion and contraction

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Product

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Ca²⁺ as a coordinator of skeletal muscle differentiation, fusion and contraction