Evolution of glucocorticoid receptors with different glucocorticoid sensitivity

Stolte, Ellen H.; Kemenade, B.M.L. Verburg-van; Savelkoul, H.F.J.; Flik, G.

doi:10.1677/joe.1.06703

Cited by 148 publications

(109 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Most fish contain two copies of this gene as a result of a genome duplication during evolution. This led to the presence of two GR isoforms: GR1 and GR2 (Stolte et al, 2006).…”

Section: Glucocorticoid Drug Screensmentioning

confidence: 99%

Zebrafish development and regeneration: new tools for biomedical research

Brittijn

Duivesteijn²,

Belmamoune³

et al. 2009

Int. J. Dev. Biol.

Self Cite

147

View full text Add to dashboard Cite

Basic research in pattern formation is concerned with the generation of phenotypes and tissues. It can therefore lead to new tools for medical research. These include phenotypic screening assays, applications in tissue engineering, as well as general advances in biomedical knowledge. Our aim here is to discuss this emerging field with special reference to tools based on zebrafish developmental biology. We describe phenotypic screening assays being developed in our own and other labs. Our assays involve: (i) systemic or local administration of a test compound or drug to zebrafish in vivo; (ii) the subsequent detection or "readout" of a defined phenotypic change. A positive readout may result from binding of the test compound to a molecular target involved in a developmental pathway. We present preliminary data on assays for compounds that modulate skeletal patterning, bone turnover, immune responses, inflammation and early-life stress. The assays use live zebrafish embryos and larvae as well as adult fish undergoing caudal fin regeneration. We describe proof-of-concept studies on the localised targeting of compounds into regeneration blastemas using microcarriers. Zebrafish are cheaper to maintain than rodents, produce large numbers of transparent eggs, and some zebrafish assays could be scaled-up into medium and high throughput screens. However, advances in automation and imaging are required. Zebrafish cannot replace mammalian models in the drug development pipeline. Nevertheless, they can provide a cost-effective bridge between cell-based assays and mammalian whole-organism models. KEY WORDS: Danio rerio, zebrafish, high-throughput screening, high-content screeningThis article is part of a special journal issue on "Pattern Formation". In this context, it seems appropriate to ask: 'what benefits to society can come from research into pattern formation?' The answer depends on how "pattern formation" is defined. For our purposes, it includes developmental mechanisms that generate Int. J. Dev. Biol. 53: 835-850 (2009) doi: 10.1387/ijdb.082615sb One is the field of human congenital malformations (the pres-836 S. A. Brittijn et al. ence at birth of anatomical abnormalities). Good examples are human malformations of fingers and toes, some cases of which are linked to mutations in the Hox family of developmental patterning genes (Akarsu et al., 1996;Goodman et al., 1997;Goodman, 2002). Teratology -the study of agents that cause congenital malformations -can be seen as applied pattern formation research. Tissue engineering, a medical research discipline that aims to create replacement tissues for patients who have suffered injury or surgical removal of tissues, is another area where knowledge of pattern formation can be applied. To date, the commonest approach to tissue engineering is to culture autologous cells on a synthetic template (Silva and Mooney, 2004). In the future, however, it may be possible to promote pattern formation by these cells so that they generate selforganised tissues or structures ...

show abstract

“…Most fish contain two copies of this gene as a result of a genome duplication during evolution. This led to the presence of two GR isoforms: GR1 and GR2 (Stolte et al, 2006).…”

Section: Glucocorticoid Drug Screensmentioning

confidence: 99%

Zebrafish development and regeneration: new tools for biomedical research

Brittijn

Duivesteijn²,

Belmamoune³

et al. 2009

Int. J. Dev. Biol.

Self Cite

147

View full text Add to dashboard Cite

show abstract

“…In the catfish, dexamethasone treatment suppressed the LPS-induced iNOS expression and NO production. Cortisol receptors have been described in fish immune cells and modulate pro-and anti-inflammatory agents (Weyts et al, 1998a;Stolte et al, 2006;Stolte et al, 2008;Teles et al, 2013). In brief, the phagocytic activity of the macrophages parallels NO production and is modulated by LPS and glucocorticoid in opposite direction.…”

Section: No Productionmentioning

confidence: 99%

In Vitro Effects of Lipopolysaccharide and Stress Hormones on Phagocytosis and Nitric Oxide Production by Enriched Head Kidney Macrophage Cultures in the Catfish

Kumar

Joy

2018

Proceedings of the Indian National Science Academy

View full text Add to dashboard Cite

Head kidney (HK) is the embryonic pronephric kidney retained in adult teleosts and contains the adrenal homologues (interrenal and chromaffin cells) and hemopoietic tissue. The adrenal homologues secrete the stress hormones glucorticoids (cortisol) and catecholamines, and the hemopoietic tissue is a major source of monocytes/macrophages and neutrophils that serve as the first line of defence against invading pathogens. In the present study, head kidney macrophage-enriched preparations of Heteropneustes fossilis were used to demonstrate the dynamics of phagocytosis and nitric oxide (NO) production in the presence of lipopolysaccharide (LPS; bacterial toxin), the synthetic glucocorticoid dexamethasone and/or catecholamines. The incubation of enriched macrophage cultures with LPS for 6 h at 20 o C stimulated phagocytosis of yeast cells and fluorescent latex beads. On the other hand, the cortisol agonist dexamethasone (10 nM) inhibited phagocytosis of yeast cells and latex beads under similar conditions. LPS stimulated inducible NO synthase (iNOS)-like expression in the macrophage cultures, which was inhibited by dexamethasone in co-incubations. Complementary to the iNOS expression, LPS stimulated NO production (nitrite level), which was inhibited by the NO synthase inhibitor L-NMMA. Dexamethasone inhibited basal as well as the LPS-induced stimulation of NO. The catecholamines epinephrine and norepinephrine did not alter the basal NO level but inhibited the LPS-induced stimulation of NO. Dopamine stimulated NO production only at a higher concentration. The results provide evidence for the existence of an endocrine-immune interaction at the level of the head kidney to modulate macrophage activity and immune functions in the catfish.

show abstract

“…In particular, the N-terminal region of this nuclear receptor is involved in the transactivation of downstream genes, and mutations in this domain decrease the transcriptional activity without affecting ligand affinity. This is considered the reason for individual variations, cortisol resistance and different regulatory functions of cortisol (Stolte et al, 2006).…”

Section: The Immune System Is Affected By the Stress Responsementioning

confidence: 99%

Immune system response to stress factors

Amadori¹,

Stefanon

Sgorlon

et al. 2009

Italian Journal of Animal Science

View full text Add to dashboard Cite

This review highlights fundamental mechanisms of the stress response and important findings as to how the immune system is affected and affects, in turn, such a response. The crucial link between stress response and energy metabolism is dealt with as well. The effector mechanisms in the stress response are remarkably similar for both infectious and non-infectious stimuli, albeit differently modulated. "Psychosensitive stimuli/behavioural response" and "Antigenic stimuli/immune response" are indeed two subsystems of a unitary, integrated complex aimed at providing optimal conditions for the host's survival and adaptation. The interaction between the immune system and the stress/inflammation complex has led to the development of a diversified network of cytokines and chemokines in vertebrate animals. The cytokine response can be mounted in different forms and extent by the host after exposure to both infectious and non-infectious stimuli. In this conceptual framework, microbial infections are just one category of stressing agents, which modulate the cytokine response for a better performance of the innate and adaptive immune responses. The response to infectious and non-infectious stress leads to a metabolic shift that enhances energy, amino acids and micronutrients consumption. The influence of each nutrient on different aspects of immune function is not easy to define, but it is becoming clear that many nutrients have defined roles in the immune response and, accordingly, their requirements are changed to support optimal immune function. Therefore, impairment of immune functions may arise from intakes of nutrients below or above these modified ranges of requirements.

show abstract

Evolution of glucocorticoid receptors with different glucocorticoid sensitivity

Cited by 148 publications

References 72 publications

Zebrafish development and regeneration: new tools for biomedical research

Zebrafish development and regeneration: new tools for biomedical research

In Vitro Effects of Lipopolysaccharide and Stress Hormones on Phagocytosis and Nitric Oxide Production by Enriched Head Kidney Macrophage Cultures in the Catfish

Immune system response to stress factors

Contact Info

Product

Resources

About