Evolution of H5N1 influenza virus through proteotyping of hemagglutinin with high resolution mass spectrometry

Ha, Ji-won; Downard, Kevin M.

doi:10.1039/c1an15354d

Cited by 13 publications

(15 citation statements)

References 39 publications

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“…Signature peptides are detected in mass spectra to enable direct surveillance, with the potential for immediate diagnosis without the need for chemical or biochemical treatments or the use of specific reagents. Signature peptides for other, less common subtypes of human and animal viruses have also been detected and can be characterized in the same manner (40,41). Another advantage of this approach is that it is open to the detection of novel virus strains that can escape detection by RT-PCR-based approaches, and it is also capable of detecting and differentiating other respiratory pathogens (26).…”

Section: Discussionmentioning

confidence: 96%

Incorporation of a Proteotyping Approach Using Mass Spectrometry for Surveillance of Influenza Virus in Cell-Cultured Strains

Fernandes

Downard

2014

J Clin Microbiol

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The reemergence of deadly pandemic influenza virus strains has necessitated the development of improved methods for rapid detection and subtyping of influenza viruses that will enable more strains to be characterized at the molecular level. Representative circulating strains of human influenza viruses from primary clinical specimens were grown in cell culture, purified through polyethylene glycol precipitation, proteolytically digested with an endoproteinase, and analyzed and identified by high-resolution mass spectrometry using unique signature peptides that are characteristic of type A H1N1 and H3N2 and type B influenza viruses. This proteotyping approach enabled circulating strains of type A influenza virus to be typed and subtyped, cocirculating seasonal and pandemic H1N1 viruses to be differentiated, and the lineage of type B viruses to be determined through single-ion detection by high-resolution mass spectrometry. Results were obtained using virus titers comparable to those used in reverse transcription (RT)-PCR assays with clinical specimens grown in cell cultures. The methodology represents a more rapid and direct approach than RT-PCR and can be integrated into existing procedures currently used for the surveillance of emerging pandemic and seasonal influenza viruses.

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Section: Discussionmentioning

confidence: 96%

Incorporation of a Proteotyping Approach Using Mass Spectrometry for Surveillance of Influenza Virus in Cell-Cultured Strains

Fernandes

Downard

2014

J Clin Microbiol

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“…The accurate mass values constitute signatures for conserved regions of IAV proteins that enable virus typing (87,88). The investigators used this approach to differentiate seasonal strains from pandemic H1N1 (89 -91) and study the evolution of H5N1 strains (92) and NA subtypes (93). They developed computer algorithms to identify virus reassortants from whole virus digests (94).…”

Section: Fig 2 Mechanisms Whereby Hemagglutinin Glycosylation Influences Iav Fitness (58)mentioning

confidence: 99%

Why Glycosylation Matters in Building a Better Flu Vaccine

Chang

Zaia

2019

Molecular & Cellular Proteomics

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Immunodominant influenza A virus (IAV) antigens mutate rapidly, allowing the virus to escape host antibodies. The question remains how to design vaccines that recognize conserved but subdominant IAV antigens for broader immune protection. Glycosylation is a mechanism whereby IAV evades the innate and adaptive immune systems. However, its influence on immunodominance remains poorly understood. Although mass spectrometry methods for identifying glycopeptides are maturing, quantifying glycosylation variation among sets of IAV mutants remains a technical challenge.

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“…This allowed for the identification of signature peptides unique to either human or avian strains isolated in Hong Kong post the 1997 pandemic, across all geographic regions, and during specific time periods. 27 To illustrate the evolution of the virus post its transmission to humans, the high resolution MALDI mass spectra of the tryptic peptides of the hemagglutinin derived from the A/Hong Kong/483/1997 and A/Hong Kong/213/2003 strains are shown in Fig. 8.…”

Section: Studying the Animal To Human Transmission Of The Influenza V...mentioning

confidence: 99%

Proteotyping for the rapid identification of influenza virus and other biopathogens

Downard

2013

Chem. Soc. Rev.

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The influenza virus is one of the most deadly infectious agents known to man and has been responsible for the deaths of some hundred million lives throughout human history. The need to rapidly and reliably survey circulating virus strains down to the molecular level is ever present. This tutorial describes the development and application of a new proteotyping approach that harnesses the power of high resolution of mass spectrometry to characterise the influenza virus, and by extension other bacterial and viral pathogens. The approach is shown to be able to type, subtype, and determine the lineage of human influenza virus strains through the detection of one or more signature peptide ions in the mass spectrum of whole virus digests. Pandemic strains can be similarly distinguished from seasonal ones, and new computer algorithms have been written to allow reassorted strains that pose the greatest pandemic risk to be rapidly identified from such datasets. The broader application of the approach is further demonstrated here for the parainfluenza virus, a virus which can be life threatening to children and presents similar clinical symptoms to influenza.

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Evolution of H5N1 influenza virus through proteotyping of hemagglutinin with high resolution mass spectrometry

Cited by 13 publications

References 39 publications

Incorporation of a Proteotyping Approach Using Mass Spectrometry for Surveillance of Influenza Virus in Cell-Cultured Strains

Incorporation of a Proteotyping Approach Using Mass Spectrometry for Surveillance of Influenza Virus in Cell-Cultured Strains

Why Glycosylation Matters in Building a Better Flu Vaccine

Proteotyping for the rapid identification of influenza virus and other biopathogens

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