Evolution of Hemocyanin Subunits in Mygalomorph Spiders. Distribution of Hemocyanin Subunits and Higher Classification of the Mygalomorphae.

Kuwada, Takao; Shiba, Hiroaki

doi:10.2108/zsj.17.517

Cited by 1 publication

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References 18 publications

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“…While we cannot rule out the possibility that we missed subunit c in all of our analyses, it is, however, also possible that the 4 · 6-mer hemocyanin of N. inaurata is in fact built by only six subunit types. We must also consider the findings of Kuwada and Sugita [36], which suggests that subunit loss and duplication may also occur in some mygalomorph spiders that are generally assumed to contain 4 · 6-mer hemocyanins [11,12].…”

Section: Structure and Subunit Composition Of N Inaurata Hemocyaninmentioning

confidence: 99%

Subunit sequences of the 4 × 6‐mer hemocyanin from the golden orb‐web spider, Nephila inaurata

Averdam

Markl

Burmester

2003

European Journal of Biochemistry

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The transport of oxygen in the hemolymph of many arthropod and mollusc species is mediated by large copperproteins that are referred to as hemocyanins. Arthropod hemocyanins are composed of hexamers and oligomers of hexamers. Arachnid hemocyanins usually form 4 · 6-mers consisting of seven distinct subunit types (termed a-g), although in some spider taxa deviations from this standard scheme have been observed. Applying immunological and electrophoretic methods, six distinct hemocyanin subunits were identified in the red-legged golden orb-web spider Nephila inaurata madagascariensis (Araneae: Tetragnathidae). The complete cDNA sequences of six subunits were obtained that corresponded to a-, b-, d-, e-, f-and g-type subunits. No evidence for a c-type subunit was found in this species. The inclusion of the N. inaurata hemocyanins in a multiple alignment of the arthropod hemocyanins and the application of the Bayesian method of phylogenetic inference allow, for the first time, a solid reconstruction of the intramolecular evolution of the chelicerate hemocyanin subunits. The branch leading to subunit a diverged first, followed by the common branch of the dimer-forming b and c subunits, while subunits d and f, as well as subunits e and g form common branches. Assuming a clock-like evolution of the chelicerate hemocyanins, a timescale for the evolution of the Chelicerata was obtained that agrees with the fossil record.

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Section: Structure and Subunit Composition Of N Inaurata Hemocyaninmentioning

confidence: 99%

Subunit sequences of the 4 × 6‐mer hemocyanin from the golden orb‐web spider, Nephila inaurata

Averdam

Markl

Burmester

2003

European Journal of Biochemistry

View full text Add to dashboard Cite

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Evolution of Hemocyanin Subunits in Mygalomorph Spiders. Distribution of Hemocyanin Subunits and Higher Classification of the Mygalomorphae.

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Cited by 1 publication

References 18 publications

Subunit sequences of the 4 × 6‐mer hemocyanin from the golden orb‐web spider, Nephila inaurata

Subunit sequences of the 4 × 6‐mer hemocyanin from the golden orb‐web spider, Nephila inaurata

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