The interstellar medium of the Milky Way and nearby disk galaxies harbours large-scale coherent magnetic fields of Microgauss strength, that can be explained via the action of a mean-field dynamo. As in our previous work, we aim to quantify dynamo effects that are selfconsistently emerging in realistic direct magnetohydrodynamic simulations, but we generalise our approach to the case of a non-local (non-instantaneous) closure relation, described by a convolution integral in space (time). To this end, we leverage our comprehensive simulation framework for the supernova-regulated turbulent multi-phase interstellar medium. By introducing spatially (temporally) modulated mean fields, we extend the previously used test-field method to the spectral realm -providing the Fourier representation of the convolution kernels. The resulting spectra of the dynamo mean-field coefficients that we obtain broadly match expectations and allow to rigorously constrain the degree of scale separation in the Galactic dynamo. A surprising result is found for the diamagnetic pumping term, which increases in amplitude when going to smaller scales. Our results amount to the most comprehensive description of dynamo mean-field effects in the Galactic context to date. Surveying the relevant parameter space and quenching behaviour, this will ultimately enable the development of assumption-free sub-grid prescriptions for otherwise unresolved global galaxy simulations.