Evolution of lymphocytes. Immunoglobulin T of the teleost sea bass ( Dicentrarchus labrax ): Quantitation of gene expressing and immunoreactive cells

Picchietti, Simona; Nuñez-Ortiz, Noelia; Stocchi, V.; Randelli, Elisa; Buonocore, Francesco; Guerra, Laura; Scapigliati, Giuseppe

doi:10.1016/j.fsi.2017.02.002

Cited by 24 publications

(16 citation statements)

References 39 publications

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“…Any immune response mounted against gliadin or some other component of wheat gluten is not likely due to an adaptive response, in this case. The size of ∼73 kDa for the heavy chains of the antibodies is similar to the size of ∼78 kDa detected by Picchietti et al (45).…”

Section: Resultssupporting

confidence: 87%

Gluten-free Fish? Marine Carnivores Cobia (Rachycentron canadum) and European Sea Bass (Dicentrarchus labrax) Have Different Tolerances to Dietary Wheat Gluten

Larkin

Watson

Place

2019

Preprint

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29 30 2 Current Address: 31 Marine Resources Research Institute 32 South Carolina Department of Natural Resources 2 33 217 Fort Johnson Rd. 34 Charleston, SC 29412 35 843-953-0462 36 watsona@dnr.sc.gov 3 37 Abstract 38In developing more sustainable fishmeal-free diets for a broad range of fish species, a 39 "one-size-fits-all" approach should not be presumed. The production of more ecologically 40 sustainable aquaculture diets has increased the incorporation of plant-based protein sources 41 such as wheat gluten. Here we show that wheat gluten at even less than 4% inclusion in a 42 compound feed has a negative impact on growth and survivorship in juvenile cobia (Rachycentron 43 canadum). In addition, plasma factors capable of binding wheat gluten were detected in the 44 plasma of cobia fed diets containing this ingredient but not in wild cobia with no exposure to 45 dietary wheat gluten. Furthermore, there is evidence that supplementary taurine partially 46 mitigates the deleterious effects provoked by wheat gluten. Based on these results, we propose 47 that wheat gluten should be added with caution to aquaculture diets intended for juvenile cobia 48 and potentially other marine carnivores. After observing that dietary wheat gluten can cause 49 deleterious effects in cobia, we sought to evaluate a possible effect in European sea bass 50 (Dicentrarchus labrax), another large, carnivorous, marine species. There were no major effects 51 in terms of growth rate, plasma biochemical parameters, or detectable induction of plasma IgM, 52 IgT, or factors capable of binding gliadin in response to 4% dietary wheat gluten. However, 53 plasma levels of taurine doubled and there were considerable changes to the intestinal 54 microbiome. There was increased diversity of predominant taxonomic orders in the pyloric caeca, 55 anterior, middle, and posterior intestinal sections of fish consuming wheat gluten. Despite these 56 measurable changes, the data suggest that dietary inclusion of 4% wheat gluten is well tolerated 57 by European sea bass in feed formulations. Together these findings underscore the need to 58 evaluate tolerance to ingredients in aquaculture formulations on a species by species basis. 4 59 60 Introduction 61Reducing fishmeal inclusion in favor of more sustainable and cost-effective protein 62 sources is a high priority for the aquaculture industry (1). Aquaculture diets that incorporate low-63 fat, high-protein concentrates derived from plants such as soy or wheat (including processed 64 wheat gluten) have been described for many species (2)(3). Feed formulations often incorporate 65 wheat gluten as a protein source and for binding and adding a desirable chewy texture to pellets.66 Wheat flour is processed to remove soluble fibers and starches, and the vital wheat gluten 67 product that remains contains two fractions: soluble gliadins and insoluble glutenins (4)(5). Vital 68 wheat gluten, marketed as a dry powder, regains its elastic properties when rehydrated (6). In 69 addition to being a low-cost protein so...

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Section: Resultssupporting

confidence: 87%

Gluten-free Fish? Marine Carnivores Cobia (Rachycentron canadum) and European Sea Bass (Dicentrarchus labrax) Have Different Tolerances to Dietary Wheat Gluten

Larkin

Watson

Place

2019

Preprint

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“…This last gene is leading the formation of Sialyl-Tn antigens in Mucin type O-glycan biosynthesis pathways (Brockhausen, 1999, Varki et al 1999. Interestingly, the alpha-Nacetylgalactosaminide alpha-2,6-sialyltransferase 1-like (st6galnac1-like) is also upregulated (Table 6, Supplemental 4 and Supplemental 12b), thus suggesting that the B-cells of gills in Mozambique tilapia may be secreting Igs, in agreement with previous studies on non-mammalian B-cell (Danilova et al, 2005;Hansen et al, 2005;Hsu et al, 2006;Picchietti et al, 2017). However, the high increase in the κlight chain and not so important upregulation in λ-light chain of immunoglobulins noticed in the Mozambique tilapia may change the ratio of the chains and also the type of Ig that is produced.…”

Section: Differences Between Nile and Mozambique Tilapias Facing 6 We...supporting

confidence: 90%

Different transcriptomic architecture of the gill epithelia in Nile and Mozambique tilapia after salinity challenge

Campo

Nitzan

Slosman

et al. 2022

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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“…Production of antigen-specific antibody in fish is known since almost 70 years, and research has shown that fish have B cells expressing three heavy Ig chain classes, namely, IgM, IgT/Z, and IgD, as defined by the expressed genes μ, τ, and δ, respectively ( 74 – 76 ), and of some Ig light chains (e.g., two in catfish, three in zebrafish, MW 25–28 kDa) ( 77 ). The IgM are tetrameric in fish (MW 450 kDa) and present systemically in body fluids, where they may be present in serum at high concentration.…”

Section: Fish Lymphocytesmentioning

confidence: 99%

Fish Lymphocytes: An Evolutionary Equivalent of Mammalian Innate-Like Lymphocytes?

2018

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Lymphocytes are the responsible of adaptive responses, as they are classically described, but evidence shows that subpopulations of mammalian lymphocytes may behave as innate-like cells, engaging non-self rapidly and without antigen presentation. The innate-like lymphocytes of mammals have been mainly identified as γδT cells and B1-B cells, exert their activities principally in mucosal tissues, may be involved in human pathologies and their functions and tissue(s) of origin are not fully understood. Due to similarities in the morphology and immunobiology of immune system between fish and mammals, and to the uniqueness of having free-living larval stages where the development can be precisely monitored and engineered, teleost fish are proposed as an experimental model to investigate human immunity. However, the homology between fish lymphocytes and mammalian innate-like lymphocytes is an issue poorly considered in comparative immunology. Increasing experimental evidence suggests that fish lymphocytes could have developmental, morphological, and functional features in common with innate-like lymphocytes of mammals. Despite such similarities, information on possible links between conventional fish lymphocytes and mammalian innate-like lymphocytes is missing. The aim of this review is to summarize and describe available findings about the similarities between fish lymphocytes and mammalian innate-like lymphocytes, supporting the hypothesis that mammalian γδT cells and B1-B cells could be evolutionarily related to fish lymphocytes.

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Evolution of lymphocytes. Immunoglobulin T of the teleost sea bass ( Dicentrarchus labrax ): Quantitation of gene expressing and immunoreactive cells

Cited by 24 publications

References 39 publications

Gluten-free Fish? Marine Carnivores Cobia (Rachycentron canadum) and European Sea Bass (Dicentrarchus labrax) Have Different Tolerances to Dietary Wheat Gluten

Gluten-free Fish? Marine Carnivores Cobia (Rachycentron canadum) and European Sea Bass (Dicentrarchus labrax) Have Different Tolerances to Dietary Wheat Gluten

Different transcriptomic architecture of the gill epithelia in Nile and Mozambique tilapia after salinity challenge

Fish Lymphocytes: An Evolutionary Equivalent of Mammalian Innate-Like Lymphocytes?

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