Evolution of Rubisco activase gene in plants

Nagarajan, R.; Gill, Kulvinder S.

doi:10.1007/s11103-017-0680-y

Cited by 46 publications

(48 citation statements)

References 70 publications

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“…To activate the enzyme, the bound RuBP must be removed by Rubisco activase, an ATPase associated with various cellular activities (AAA+) protein, in an ATP‐dependent reaction . Although the C‐terminal His6‐tag on RbcS did not impede the assembly and the carboxylation activity of the recombinant Rubisco, we next investigated whether the His6‐tag interferes with the recognition and subsequent reactivation of the inhibited enzyme by A. thaliana Rubisco activase β‐isoform ( At Rcaβ) . The uncarbamylated At RbcL 8 S 8 His6 , like the leaf synthesized At RbcL 8 S 8 , could be inhibited by RuBP (EI), showing essentially no CO 2 fixation as a function of time (Fig.…”

Section: Resultsmentioning

confidence: 99%

Improved recombinant expression and purification of functional plant Rubisco

et al. 2019

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Improving the performance of the key photosynthetic enzyme Ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco) by protein engineering is a critical strategy for increasing crop yields. The extensive chaperone requirement of plant Rubisco for folding and assembly has long been an impediment to this goal. Production of plant Rubisco in Escherichia coli requires the coexpression of the chloroplast chaperonin and four assembly factors. Here, we demonstrate that simultaneous expression of Rubisco and chaperones from a T7 promotor produces high levels of functional enzyme. Expressing the small subunit of Rubisco with a C‐terminal hexahistidine‐tag further improved assembly, resulting in a ~ 12‐fold higher yield than the previously published procedure. The expression system described here provides a platform for the efficient production and engineering of plant Rubisco.

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Section: Resultsmentioning

confidence: 99%

Improved recombinant expression and purification of functional plant Rubisco

et al. 2019

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“…However, acclimation to warmer growing conditions can increase the optimum temperature of photosynthesis in wheat (Yamasaki et al, 2002), suggesting longer-term biochemical adjustments to higher temperatures. In many species, including rice, there is a single functional Rca gene that encodes two protein isoforms based on alternative splicing of pre-mRNA into a larger a-isoform and a shorter b-isoform (To et al, 1999;Nagarajan and Gill, 2018). Similarly, for wheat, there is an aand a b-isoform, but wheat has an additional Rca gene that encodes a second variant of the b-isoform.…”

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confidence: 99%

“…Similarly, for wheat, there is an aand a b-isoform, but wheat has an additional Rca gene that encodes a second variant of the b-isoform. The latter gene is referred to as form 1 and the former alternatively spliced gene is referred to as form 2 (Carmo-Silva et al, 2015;Nagarajan and Gill, 2018). Thus, in wheat, excluding the nearly identical copies on each of the three wheat chromosome subgenomes, there are three distinct Rca gene products that can be expressed: a form 1b (TaRca1-b), form 2a (TaRca2-a), and form 2b (TaRca2-b).…”

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confidence: 99%

A Conserved Sequence from Heat-Adapted Species Improves Rubisco Activase Thermostability in Wheat

Scafaro

Bautsoens²,

Boer³

et al. 2019

Plant Physiol.

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The central enzyme of photosynthesis, Rubisco, is regulated by Rubisco activase (Rca). Photosynthesis is impaired during heat stress, and this limitation is often attributed to the heat-labile nature of Rca. We characterized gene expression and protein thermostability for the three Rca isoforms present in wheat (Triticum aestivum), namely TaRca1-b, TaRca2-a, and TaRca2-b. Furthermore, we compared wheat Rca with one of the two Rca isoforms from rice (Oryza sativa; OsRca-b) and Rca from other species adapted to warm environments. The TaRca1 gene was induced, whereas TaRca2 was suppressed by heat stress. The TaRca2 isoforms were sensitive to heat degradation, with thermal midpoints of 35°C 6 0.3°C, the temperature at which Rubisco activation velocity by Rca was halved. By contrast, TaRca1-b was more thermotolerant, with a thermal midpoint of 42°C, matching that of rice OsRca-b. Mutations of the TaRca2-b isoform based on sequence alignment of the thermostable TaRca1b from wheat, OsRca-b from rice, and a consensus sequence representing Rca from warm-adapted species enabled the identification of 11 amino acid substitutions that improved its thermostability by greater than 7°C without a reduction in catalytic velocity at a standard 25°C. Protein structure modeling and mutational analysis suggested that the thermostability of these mutational variants arises from monomeric and not oligomeric thermal stabilization. These results provide a mechanism for improving the heat stress tolerance of photosynthesis in wheat and potentially other species, which is a desirable outcome considering the likelihood that crops will face more frequent heat stress conditions over the coming decades.

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“…Carboxylation is the rst step in the CO 2 assimilation process. Ribulose-1,5-bis-phosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) is the key enzyme in this process, which links organic and inorganic matter in the biosphere [59]. Two DEGs (Cluster-10319.94590 and Cluster-10319.106159) encoding Rubisco were upregulated in the owers of NaCl-treated plants (Fig.…”

Section: Degs Related To Carbon Utilization and Sucrose And Starch Mementioning

confidence: 99%

NaCl improves reproduction by enhancing starch accumulation in the ovules of the euhalophyte Suaeda salsa

Guo

et al. 2020

Preprint

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Background: Halophytes show optimal reproduction under high-salinity conditions. However, the role of NaCl in reproduction and its possible mechanisms in the euhalophyte Suaeda salsa remain to be elucidated. Results: We performed transcript profiling of S. salsa flowers and measured starch accumulation in ovules, sugar contents in flowers, and photosynthetic parameters in the leaves of plants supplied with 0 and 200 mM NaCl. Starch accumulation in ovules, sugar contents in flowers and ovules, and net photosynthetic rate and photochemical efficiency in leaves were significantly higher in NaCl-treated plants vs. the control. We identified 14,348 differentially expressed genes in flowers of NaCl-treated vs. control plants. Many of these genes were predicted to be associated with photosynthesis, carbon utilization, and sugar and starch metabolism. These genes are crucial for maintaining photosystem structure, regulating electron transport, and improving photosynthetic efficiency in NaCl-treated plants. In addition, genes encoding fructokinase and sucrose phosphate synthase were upregulated in flowers of NaCl-treated plants. Conclusions: The higher starch and sugar contents in the ovules and flowers of S. salsa in response to NaCl treatment are likely due to the upregulation of genes involved in photosynthesis and carbohydrate metabolism, which increase photosynthetic efficiency and accumulation of photosynthetic products under these conditions.

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Evolution of Rubisco activase gene in plants

Cited by 46 publications

References 70 publications

Improved recombinant expression and purification of functional plant Rubisco

Improved recombinant expression and purification of functional plant Rubisco

A Conserved Sequence from Heat-Adapted Species Improves Rubisco Activase Thermostability in Wheat

NaCl improves reproduction by enhancing starch accumulation in the ovules of the euhalophyte Suaeda salsa

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