Evolution of Shock-Induced Pressure in Laser Bioprinting

Mareev, E. I.; Минаев, Н. В.; Zhigarkov, V. S.; Yusupov, V. I.

doi:10.3390/photonics8090374

Cited by 12 publications

(4 citation statements)

References 38 publications

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“…During the laser bioprinting process, microorganisms are subjected to various shocks and chronic effects. Shock effects are caused by pulse laser irradiation [ 20 ], shock waves [ 21 ], temperature change [ 22 ], and impulsive pressure gradients associated with acceleration near the donor plate and landing on the acceptor surface [ 23 ]. Chronic effects on the microorganisms are due to the effects of the gel and its potential modifications due to laser exposure and nanoparticles from the destroyed area of the absorbing film [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting

Grosfeld

Zhigarkov

Alexandrov

et al. 2022

IJMS

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Laser-induced forward transfer (LIFT) is a useful technique for bioprinting using gel-embedded cells. However, little is known about the stresses experienced by cells during LIFT. This paper theoretically and experimentally explores the levels of laser pulse irradiation and pulsed heating experienced by yeast cells during LIFT. It has been found that only 5% of the cells in the gel layer adjacent to the absorbing Ti film should be significantly heated for fractions of microseconds, which was confirmed by the fact that a corresponding population of cells died during LIFT. This was accompanied by the near-complete dimming of intracellular green fluorescent protein, also observed in response to heat shock. It is shown that microorganisms in the gel layer experience laser irradiation with an energy density of ~0.1–6 J/cm2. This level of irradiation had no effect on yeast on its own. We conclude that in a wide range of laser fluences, bioprinting kills only a minority of the cell population. Importantly, we detected a previously unobserved change in membrane permeability in viable cells. Our data provide a wider perspective on the effects of LIFT-based bioprinting on living organisms and might provide new uses for the procedure based on its effects on cell permeability.

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Section: Introductionmentioning

confidence: 99%

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting

Grosfeld

Zhigarkov

Alexandrov

et al. 2022

IJMS

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“…The liquid phase is present surrounding the vapor bubble in the remaining donor material. The formation of vapor can cause a sudden pressure rise in the donor film [32]. The effect of sudden pressure rise in the donor on the LIFT process is ignored in this model as it mainly deals with thermal aspects.…”

Section: Jet Ejectionmentioning

confidence: 99%

Numerical modeling to predict threshold fluence for material ejection in Laser-Induced forward transfer of metals

Muniyallappa,

Chandra,

Marla

2023

Phys. Scr.

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Laser-induced forward transfer (LIFT) uses a pulsed laser beam to propel material from a donor (containing a glass coated with a thin material film) onto a receiving substrate, resulting in pixellated material deposition. The deposition characteristics depend on the material ejection modes that vary with film thickness and laser parameters. This study develops a computational model based on thefinite volume method for LIFT of gold films using nanosecond pulses, which captures two different ejection modes for droplet deposition —cap ejection and jet ejection. The model computes the temperature distribution and predicts potential ejection regimes for different film thicknesses, providing an understanding of the material removal process. Cap ejection occurs at lower laser fluences whenthe entire film thickness in the irradiated zone is in the molten phase. In comparison, jet ejection occurs at higher laser fluences caused by vapor pocket formation at the glass-film interface. The model predicts threshold fluences with greater than 90% accuracy for film thickness less than 583 nm. However, for the film thickness of more than 583 nm, the simulations underpredict the threshold fluence, suggesting that laser absorption by the film decreases due to vapor formation at the glass-film interface. Besides, it is observed that higher fluences can cause melting and vaporization of the glass at the interface leading to possible contamination of the deposited material. The proposed model can be used to choose the operating window for laser parameters for droplet deposition in LIFT, which otherwise is challenging using experiments.

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“…The first one made it possible to move the donor plate with spheroids deposited on it, placing the spheroids in the area of laser radiation focusing. The second one was used to displace the acceptor In the case of using a laser spot with a Gaussian intensity distribution and a small waist radius (TG), a rapidly expanding region with a vapor-gas bubble [13,16] with transverse dimensions smaller than the spheroid diameter is formed in the region above the spheroid top (Figure 8). The resulting high values of the pressure gradient associated with the high irregularity of the distribution of the intensity of the laser impact, in this case, will lead to the destruction of the spheroid.…”

Section: Advantages and Disadvantages Of Different Lift Modesmentioning

confidence: 99%

“…Laser-induced forward transfer (LIFT) [ 11 , 12 , 13 , 14 ] is another bioprinting technique that can surpass the above-mentioned approaches and overcome their drawbacks. Applying cell suspensions, it has shown the ability to print the constructs with micron-scale precision and at high speed from the bioinks with a wide viscosity range [ 15 ] while supporting sufficient post-printing cell viability, which makes this technique a candidate for spheroid bioprinting [ 16 , 17 , 18 , 19 ]. However, LIFT has not been applied for this purpose yet.…”

Section: Introductionmentioning

confidence: 99%

Laser Bioprinting with Cell Spheroids: Accurate and Gentle

et al. 2023

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Laser printing with cell spheroids can become a promising approach in tissue engineering and regenerative medicine. However, the use of standard laser bioprinters for this purpose is not optimal as they are optimized for transferring smaller objects, such as cells and microorganisms. The use of standard laser systems and protocols for the transfer of cell spheroids leads either to their destruction or to a significant deterioration in the quality of bioprinting. The possibilities of cell spheroids printing by laser-induced forward transfer in a gentle mode, which ensures good cell survival ~80% without damage and burns, were demonstrated. The proposed method showed a high spatial resolution of laser printing of cell spheroid geometric structures at the level of 62 ± 33 µm, which is significantly less than the size of the cell spheroid itself. The experiments were performed on a laboratory laser bioprinter with a sterile zone, which was supplemented with a new optical part based on the Pi-Shaper element, which allows for forming laser spots with different non-Gaussian intensity distributions. It is shown that laser spots with an intensity distribution profile of the “Two rings” type (close to Π-shaped) and a size comparable to a spheroid are optimal. To select the operating parameters of laser exposure, spheroid phantoms made of a photocurable resin and spheroids made from human umbilical cord mesenchymal stromal cells were used.

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Evolution of Shock-Induced Pressure in Laser Bioprinting

Cited by 12 publications

References 38 publications

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting

Numerical modeling to predict threshold fluence for material ejection in Laser-Induced forward transfer of metals

Laser Bioprinting with Cell Spheroids: Accurate and Gentle

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