Evolution of symbiosis in the Vibrionaceae: a combined approach using molecules and physiology

Nishiguchi, Michele K.; Nair, Vinod S.

doi:10.1099/ijs.0.02792-0

Cited by 61 publications

(70 citation statements)

References 46 publications

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“…In addition, members of the Vibrionaceae occur naturally in the digestive tract and on the skin surface of marine animals [1]. In general, the genus Vibrio -along with their close relatives such as Photobacterium-are thought to be especially adapted to engaging in pathogenic and benign host-microbe interactions, with these symbiosis traits probably having a deep and ancient common ancestry, arising independently numerous times during the evolution of Vibrionaceae [5].…”

Section: Introductionmentioning

confidence: 99%

“…V. fischeri isolated from monocentrid fishes are only capable of colonizing sepiolid squids in laboratory experiments at a reduced efficiency and possess a lower carrying capacity within cephalopod hosts [9]. Prior data has also demonstrated that symbiotically incompetent V. fischeri and those colonizing Euprymna, Sepiola, and monocentrid fishes are genetically distinct from each other [5,[10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Salinity and Temperature Effects on Physiological Responses of Vibrio fischeri from Diverse Ecological Niches

Soto

Gutiérrez

Remmenga³

et al. 2008

Microb Ecol

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100

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Vibrio fischeri is a bioluminescent bacterial symbiont of sepiolid squids (Cephalopoda: Sepiolidae) and monocentrid fishes (Actinopterygii: Monocentridae). V. fischeri exhibit competitive dominance within the allopatrically distributed squid genus Euprymna, which have led to the evolution of V. fischeri host specialists. In contrast, the host genus Sepiola contains sympatric species that is thought to have given rise to V. fischeri that have evolved as host generalists. Given that these ecological lifestyles may have a direct effect upon the growth spectrum and survival limits in contrasting environments, optimal growth ranges were obtained for numerous V. fischeri isolates from both freeliving and host environments. Upper and lower limits of growth were observed in sodium chloride concentrations ranging from 0.0% to 9.0%. Sepiola symbiotic isolates possessed the least variation in growth throughout the entire salinity gradient, whereas isolates from Euprymna were the least uniform at <2.0% NaCl. V. fischeri fish symbionts (CG101 and MJ101) and all free-living strains were the most dissimilar at >5.0% NaCl. Growth kinetics of symbiotic V. fischeri strains were also measured under a range of salinity and temperature combinations. Symbiotic V. fischeri ES114 and ET101 exhibited a synergistic effect for salinity and temperature, where significant differences in growth rates due to salinity existed only at low temperatures. Thus, abiotic factors such as temperature and salinity have differential effects between free-living and symbiotic strains of V. fischeri, which may alter colonization efficiency prior to infection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Salinity and Temperature Effects on Physiological Responses of Vibrio fischeri from Diverse Ecological Niches

Soto

Gutiérrez

Remmenga³

et al. 2008

Microb Ecol

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100

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“…Vibrio isolate T5 as well as the other disease causing Vibrio isolates T2 and T3 shared 99% similarity of their 16S rRNA gene sequence with V. parahaemolyticus AY303956 (Nishiguchi & Nair 2003). Yet, a cladogram based on type strains of Vibrio spp.…”

Section: Taxonomic Classification Of Puws Causing Isolatementioning

confidence: 99%

Vibrio sp. causing Porites ulcerative white spot disease

Arboleda¹,

Reichardt²

2010

Dis. Aquat. Org.

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The causative agent of the Indo-Pacific coral disease, Porites ulcerative white spot syndrome (PUWS), that affects Porites spp. and a few other coral genera has so far remained unidentified. Inoculation of thiosulphate citrate bile sucrose (TCBS) agar with tissue material from Porites cylindrica infected with white spot produced colonies of approximately 3 mm diameter consisting of Gram-negative, motile, non-sucrose-fermenting, slightly curved rods with a minimum NaCl requirement of 0.3%. Three of these putative Vibrio sp. isolates were used for infection trials that included different stages of cell growth. Four modes of inoculation and 3 stages of bacterial cell growth were considered for testing Koch's postulates. Stationary phase cells proved more consistently infectious than did exponentially growing or starved cells using a 1-step immersion technique at cell concentrations of 10 4 cells ml -1. A 1-step immersion technique proved more reliable in producing signs of white spot than did other techniques, such as injection, smearing and 2-step immersion of the inoculum. At inoculum densities >10 4 cells ml -1 further signs of disease, such as tissue degradation and bleaching, also became evident. At elevated temperatures (> 29°C) bleaching remained absent for at least 2 mo from non-inoculated corals serving as controls, but was observed in artificially infected coral fragments. Of the 9 seawater aquaria containing healthy specimens of P. cylindrica, 6 showed signs of white spot 15 d after infection with an isolate tentatively identified as Vibrio sp. Based on 99% similarity of its 16S rRNA gene sequence and selected phenotypical features, this isolate revealed a close relationship to V. natriegens and V. parahaemolyticus. KEY WORDS: Indopacific coral disease · Porites · Causative agent · Vibrio Resale or republication not permitted without written consent of the publisherDis Aquat Org 90: [93][94][95][96][97][98][99][100][101][102][103][104] 2010 ora spp., Montastrea spp. and Pocillopora damicornis (Rosenberg et al. 2007). Vibrio species are widespread copiotropic constituents of mesophilic microbial communities that favor organic pollution from shellfish and fish cultivation in coastal marine environments (Colwell et al. 1977, Colwell 1996, Reichardt et al. 2007). This group also harbors the causative agents of an extremely wide range of diseases of commercial marine fishes and shellfish (Bruno et al. 1998, Robertson et al. 1998, Lacoste et al. 2001). At year-round surface water temperatures between 27 and 33°C coral reefs in the vicinity of intensive milkfish Chanos chanos mariculture at Cape Bolinao (Lingayen Gulf, Philippines) are particularly exposed to high densities of different populations of Vibrio spp. (W. T. Reichardt et al. unpubl. data). Thus, a highly selective enrichment medium for marine vibrios, such as thiosulphate citrate bile sucrose (TCBS) agar (Bolinches et al.1988), was considered as first choice for isolating test strains from white spots of diseased Porites cylindrica to ...

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“…Different loci, e.g., 23S rRNA (21), gapA (23), gyrB (20), hsp60 (18), and recA (30), have been used for phylogenetic studies and the identification of Vibrionaceae species. So far, these genes (except for recA) have only been examined in a very limited number of species and strains.…”

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confidence: 99%

Phylogeny and Molecular Identification of Vibrios on the Basis of Multilocus Sequence Analysis

Thompson

Gevers

Thompson

et al. 2005

Appl Environ Microbiol

413

381

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We analyzed the usefulness of rpoA, recA, and pyrH gene sequences for the identification of vibrios. We sequenced fragments of these loci from a collection of 208 representative strains, including 192 well-documented Vibrionaceae strains and 16 presumptive Vibrio isolates associated with coral bleaching. In order to determine the intraspecies variation among the three loci, we included several representative strains per species. The phylogenetic trees constructed with the different genetic loci were roughly in agreement with former polyphasic taxonomic studies, including the 16S rRNA-based phylogeny of vibrios. The families Vibrionaceae, Photobacteriaceae, Enterovibrionaceae, and Salinivibrionaceae were all differentiated on the basis of each genetic locus. Each species clearly formed separated clusters with at least 98, 94, and 94% rpoA, recA, and pyrH gene sequence similarity, respectively. The genus Vibrio was heterogeneous and polyphyletic, with Vibrio fischeri, V. logei, and V. wodanis grouping closer to the Photobacterium genus. V. halioticoli-, V. harveyi-, V. splendidus-, and V. tubiashii-related species formed groups within the genus Vibrio. Overall, the three genetic loci were more discriminatory among species than were 16S rRNA sequences. In some cases, e.g., within the V. splendidus and V. tubiashii group, rpoA gene sequences were slightly less discriminatory than recA and pyrH sequences. In these cases, the combination of several loci will yield the most robust identification. We can conclude that strains of the same species will have at least 98, 94, and 94% rpoA, recA, and pyrH gene sequence similarity, respectively.Vibrios are gram-negative, usually motile rods, are mesophilic and chemoorganotrophic, and have a facultatively fermentative metabolism (5). They are generally able to grow on marine agar and on the selective medium thiosulfate-citratebile salt-sucrose agar and are mostly oxidase positive. Vibrios belong to the Gammaproteobacteria according to 16S rRNA gene sequence analysis. These bacteria are found abundantly in aquatic habitats and in association with eukaryotes. Associations established by vibrios range from mutualistic, e.g., Vibrio fischeri-bobtail squid (26), to pathogenic, e.g., V. cholerae-humans (45). Probiotic Vibrio strains for fish and shellfish have also been documented (44).The current family Vibrionaceae comprises the genera Enterovibrio (2 species), Grimontia (1 species), Photobacterium (7 species), Salinivibrio (1 species), and Vibrio (64 species). The novel species Photobacterium rosenbergii and Enterovibrio coralii have recently been proposed to encompass isolates associated with coral bleaching (41). Several new Vibrio species, mainly in the phylogenetic neighborhood of V. harveyi, V. halioticoli, V. splendidus, V. tubiashii, and V. fluvialis, have been described in the last few years, with V. neonatus, V. ezurae (28), and V. ponticus (22) being the most recent ones. V. harveyi, V. splendidus, and V. tubiashii are frequently associated with disease in different spe...

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Evolution of symbiosis in the Vibrionaceae: a combined approach using molecules and physiology

Cited by 61 publications

References 46 publications

Salinity and Temperature Effects on Physiological Responses of Vibrio fischeri from Diverse Ecological Niches

Salinity and Temperature Effects on Physiological Responses of Vibrio fischeri from Diverse Ecological Niches

Vibrio sp. causing Porites ulcerative white spot disease

Phylogeny and Molecular Identification of Vibrios on the Basis of Multilocus Sequence Analysis

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