Evolution of the 14–3–3 gene family in monocotyledons and dicotyledons and validation of MdGRF13 function in transgenic Arabidopsis thaliana

Ren, Jiaxuan; Zhang, Pan; Dai, Yingbao; Liu, Xiaohuan; Lu, Shixiong; Guo, Lili; Gou, Huimin; Mao, Juan

doi:10.1007/s00299-023-03035-4

Cited by 14 publications

(10 citation statements)

References 41 publications

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“…Consequently, lower accumulations of IL (an index of membrane injury), MDA (an index of ROS-mediated lipid peroxidation injury in the membrane), and H 2 O 2 were detected, which indicated that cell membrane stability was strengthened in BdGF14g-overexpressing tobacco plants under drought stress than in WT and VC plants (Figure 3A-H). The above results, in line with those of MdGRF11 and MdGRF13, two 14-3-3 proteins detected in apples under abiotic stress in transgenic plants [47,49], indicate that BdGF14g enhanced antioxidant capacity to improve the ROS-scavenging system under drought conditions.…”

Section: Discussionsupporting

confidence: 80%

“…BdGF14g can be upregulated by PEG and ABA and downregulated by H 2 O 2 , suggesting that BdGF14g possesses potential functions in response to these stresses [30,45,46]. Overexpressing BdGF14a and MdGRF13 exhibited enhanced resistance to drought stress in Arabidopsis [46,47]. Likewise, in transgenic tobacco, the function of BdGF14g in abiotic stress was first confirmed by the improved drought-tolerant phenotype in the young and adult seedling stages, with a better growth status, higher survival rate, higher RWC, and longer roots (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

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A 14-3-3 Protein-Encoding Gene, BdGF14g, Confers Better Drought Tolerance by Regulating ABA Biosynthesis and Signaling

Zhang,

He,

Zhao

et al. 2023

Plants

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Abscisic acid (ABA), a phytohormone, enacts a cardinal function in coping with abiotic stress. 14-3-3 proteins can interact with ABA-responsive-element-binding transcription factors (ABFs), a chief constituent of ABA signaling, and play critical roles in the dehydration response involving ABA signaling. Meanwhile, whether and how 14-3-3 proteins regulate ABA signaling to respond to aridity stress is yet to be fully investigated. Herein, BdGF14g, a 14-3-3 gene induced by ABA, H2O2, and PEG treatments, was identified in Brachypodium distachyon (B. distachyon). Overexpression of BdGF14g improved drought stress tolerance in tobacco plants, with a higher survival rate, longer root length, enhanced cell membrane stability, and increased antioxidase activity compared with non-transgenic controls in coping with dehydration. Both drought and exogenous ABA treatments resulted in smaller stomatal apertures in BdGF14g-transgenic lines. Additionally, when an ABA biosynthesis inhibitor was added, the better growth statuses, less H2O2 accumulation, and higher activities of catalase and superoxide dismutase under mannitol stress disappeared. Moreover, BdGF14g interacted with NtABF2, upregulated the endogenous ABA content, and enhanced the transcription of ABA-related genes, including NtNCED1, a crucial ABA biosynthesis gene, under drought conditions. In conclusion, BdGF14g acts as a positive factor in the water deficiency response by affecting ABA biosynthesis and signaling in tobacco plants.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

A 14-3-3 Protein-Encoding Gene, BdGF14g, Confers Better Drought Tolerance by Regulating ABA Biosynthesis and Signaling

Zhang,

He,

Zhao

et al. 2023

Plants

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“…Then, we used the real time fluorescence PCR (LightCycler® 96 Real-Time PCR System, Roche, Switzerland) to detect the expression levels of genes. The reaction system was 20 µL, containing 6 µL ddH 2 O, 2 µL cDNA, 1 µL each of upstream and downstream primers and 10 µL SYBR Green (Accurate Biology), and the reaction procedure has been described in detail by Ren et al [ 62 ]. The relative expression levels of genes were calculated using 2 −∆∆CT method [ 60 ], and the reference gene was GADPH .…”

Section: Methodsmentioning

confidence: 99%

Whole-genome resequencing and transcriptome analyses of four generation mutants to reveal spur-type and skin-color related genes in apple (Malus domestica Borkh. Cv. Red delicious)

Ren,

Li,

Guo

et al. 2023

BMC Plant Biol

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Background Bud sport is a kind of somatic mutation that usually occurred in apple. ‘Red Delicious’ is considered to be a special plant material of bud sport, whereas the genetic basis of plant mutants is still unknown. In this study, we used whole-genome resequencing and transcriptome sequencing to identify genes related to spur-type and skin-color in the ‘Red Delicious’ (G0) and its four generation mutants including ‘Starking Red’ (G1), ‘Starkrimson’ (G2), ‘Campbell Redchief’ (G3) and ‘Vallee Spur’ (G4). Results The number of single nucleotide polymorphisms (SNPs), insertions and deletions (InDels) and structural variations (SVs) were decreased in four generation mutants compared to G0, and the number of unique SNPs and InDels were over 9-fold and 4-fold higher in G1 versus (vs.) G2 and G2 vs. G3, respectively. Chromosomes 2, 5, 11 and 15 carried the most SNPs, InDels and SVs, while chromosomes 1 and 6 carried the least. Meanwhile, we identified 4,356 variation genes by whole-genome resequencing and transcriptome, and obtained 13 and 16 differentially expressed genes (DEGs) related to spur-type and skin-color by gene expression levels. Among them, DELLA and 4CL7 were the potential genes that regulate the difference of spur-type and skin-color characters, respectively. Conclusions Our study identified potential genes associated with spur-type and skin-color differences in ‘Red Delicious’ and its four generation mutants, which provides a theoretical foundation for the mechanism of the apple bud sport.

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“…Screened potential protein sequences were cloned into pGADT7 and fused to activation domain sequences to generate prey construct. Yeast transformation and point-to-point verification of interaction proteins refer to the methods of Ren et al [65].…”

Section: Y2h Protein Binding Assaymentioning

confidence: 99%

VvJAZ13 Positively Regulates Cold Tolerance in Arabidopsis and Grape

Che,

Lu,

Gou

et al. 2024

IJMS

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Cold stress adversely impacts grape growth, development, and yield. Therefore, improving the cold tolerance of grape is an urgent task of grape breeding. The Jasmonic acid (JA) pathway responsive gene JAZ plays a key role in plant response to cold stress. However, the role of JAZ in response to low temperatures in grape is unclear. In this study, VvJAZ13 was cloned from the ‘Pinot Noir’ (Vitis vinefera cv. ‘Pinot Noir’) grape, and the potential interacting protein of VvJAZ13 was screened by yeast two-hybrid (Y2H). The function of VvJAZ13 under low temperature stress was verified by genetic transformation. Subcellular localization showed that the gene was mainly expressed in cytoplasm and the nucleus. Y2H indicated that VvF-box, VvTIFY5A, VvTIFY9, Vvbch1, and VvAGD13 may be potential interacting proteins of VvJAZ13. The results of transient transformation of grape leaves showed that VvJAZ13 improved photosynthetic capacity and reduced cell damage by increasing maximum photosynthetic efficiency of photosystem II (Fv/Fm), reducing relative electrolyte leakage (REL) and malondialdehyde (MDA), and increasing proline content in overexpressed lines (OEs), which played an active role in cold resistance. Through the overexpression of VvJAZ13 in Arabidopsis thaliana and grape calli, the results showed that compared with wild type (WT), transgenic lines had higher antioxidant enzyme activity and proline content, lower REL, MDA, and hydrogen peroxide (H2O2) content, and an improved ability of scavenging reactive oxygen species. In addition, the expression levels of CBF1-2 and ICE1 genes related to cold response were up-regulated in transgenic lines. To sum up, VvJAZ13 is actively involved in the cold tolerance of Arabidopsis and grape, and has the potential to be a candidate gene for improving plant cold tolerance.

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Evolution of the 14–3–3 gene family in monocotyledons and dicotyledons and validation of MdGRF13 function in transgenic Arabidopsis thaliana

Cited by 14 publications

References 41 publications

A 14-3-3 Protein-Encoding Gene, BdGF14g, Confers Better Drought Tolerance by Regulating ABA Biosynthesis and Signaling

A 14-3-3 Protein-Encoding Gene, BdGF14g, Confers Better Drought Tolerance by Regulating ABA Biosynthesis and Signaling

Whole-genome resequencing and transcriptome analyses of four generation mutants to reveal spur-type and skin-color related genes in apple (Malus domestica Borkh. Cv. Red delicious)

VvJAZ13 Positively Regulates Cold Tolerance in Arabidopsis and Grape

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