Evolution of the complement system in protostomes revealed by de novo transcriptome analysis of six species of Arthropoda

Sekiguchi, Reo; Nonaka, Masaru

doi:10.1016/j.dci.2014.12.008

Cited by 26 publications

(28 citation statements)

References 42 publications

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“…These findings support the existence of at least some core components of a protocomplement pathway in bivalves. Likewise, the existence of a primitive complement system resembling the alternative pathway of the mammalian complement system has been recently demonstrated in other protostomes, namely in some arthropods [84].…”

Section: Evidence Of An Ancient Bivalve Complement-like Systemmentioning

confidence: 98%

An updated molecular basis for mussel immunity

Gerdol

Venier

2015

Fish & Shellfish Immunology

134

163

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Non-self recognition with the consequent tolerance or immune reaction is a crucial process to succeed as living organisms. At the same time the interactions between host species and their microbiome, including potential pathogens and parasites, significantly contribute to animal life diversity. Marine filter-feeding bivalves, mussels in particular, can survive also in heavily anthropized coastal waters despite being constantly surrounded by microorganisms. Based on the first outline of the Mytilus galloprovincialis immunome dated 2011, the continuously growing transcript data and the recent release of a draft mussel genome, we explored the available sequence data and scientific literature to reinforce our knowledge on the main gene-encoded elements of the mussel immune responses, from the pathogen recognition to its clearance. We carefully investigated molecules specialized in the sensing and targeting of potential aggressors, expected to show greater molecular diversification, and outlined, whenever relevant, the interconnected cascades of the intracellular signal transduction. Aiming to explore the diversity of extracellular, membrane-bound and intracellular pattern recognition receptors in mussel, we updated a highly complex immune system, comprising molecules which are described here in detail for the first time (e.g. NOD-like receptors) or which had only been partially characterized in bivalves (e.g. RIG-like receptors). Overall, our comparative sequence analysis supported the identification of over 70 novel full-length immunity-related transcripts in M. galloprovincialis. Nevertheless, the multiplicity of gene functions relevant to immunity, the involvement of part of them in other vital processes, and also the lack of a refined mussel genome make this work still not-exhaustive and support the development of more specific studies.

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Section: Evidence Of An Ancient Bivalve Complement-like Systemmentioning

confidence: 98%

An updated molecular basis for mussel immunity

Gerdol

Venier

2015

Fish & Shellfish Immunology

134

163

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“…; Frias‐Lopez et al . ; Sekiguchi & Nonaka ). This is presumably due to the large size of the spider genome; the average C‐value of the 115 spiders listed in the animal genome size database (Gregory ) is 2.4 ± 1.1, which is comparable to that of mammalian genomes (3.2 ± 0.85, N = 777).…”

Section: Introductionmentioning

confidence: 99%

“…), Macrothele calpeiana (Frias‐Lopez et al . ) and Hasarius adansoni (Sekiguchi & Nonaka ; Croucher et al . ; He et al .…”

Section: Introductionmentioning

confidence: 99%

“…However, spiders are group of arthropods yet to be explored comprehensively using molecular genomic approaches. To date only two genome assemblies are available, namely for Stegodyphus mimosarum and Acanthoscurria geniculata (Sanggaard et al 2014), and other studies utilize de novo transcriptome assemblies as an alternative approach for comprehensive molecular studies: Theridion grallator and T. californicum (Croucher et al 2013), Latrodectus tredecimguttatus (He et al 2013), Latrodectus hesperus (Clarke et al 2014), Parasteatoda tepidariorum (Posnien et al 2014), Gasteracantha arcuata, Nasoonaria sinensis and Gasteracantha hasselti (Zhao et al 2014), Macrothele calpeiana (Frias-Lopez et al 2015) and Hasarius adansoni (Sekiguchi & Nonaka 2015;Croucher et al 2013;He et al 2013;Clarke et al 2014;Posnien et al 2014;Zhao et al 2014;Frias-Lopez et al 2015;Sekiguchi & Nonaka 2015). This is presumably due to the large size of the spider genome; the average C-value of the 115 spiders listed in the ANIMAL GENOME SIZE DATABASE (Gregory 2015) is 2.4 AE 1.1, which is comparable to that of mammalian genomes (3.2 AE 0.85, N = 777).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly

Kono

Nakamura

Ito

et al. 2015

Molecular Ecology Resources

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With advances in high-throughput sequencing technologies, de novo transcriptome sequencing and assembly has become a cost-effective method to obtain comprehensive genetic information of a species of interest, especially in nonmodel species with large genomes such as spiders. However, high-quality RNA is essential for successful sequencing, and sample preservation conditions require careful consideration for the effective storage of field-collected samples. To this end, we report a streamlined feasibility study of various storage conditions and their effects on de novo transcriptome assembly results. The storage parameters considered include temperatures ranging from room temperature to -80°C; preservatives, including ethanol, RNAlater, TRIzol and RNAlater-ICE; and sample submersion states. As a result, intact RNA was extracted and assembly was successful when samples were preserved at low temperatures regardless of the type of preservative used. The assemblies as well as the gene expression profiles were shown to be robust to RNA degradation, when 30 million 150-bp paired-end reads are obtained. The parameters for sample storage, RNA extraction, library preparation, sequencing and in silico assembly considered in this work provide a guideline for the study of field-collected samples of spiders.

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“…Ammothea sp e no bivalve Ruditapes decussatus (PADRO-ALVAREZ et al, 2009;NONAKA, 2015). O fator B pertence à classe de peptidases do tipo A, uma vez que apresenta um domínio SP com uma conformação característica da família das quimiotripsinas, sendo que quase todos os representantes desta classe utilizam a tríade catalítica composta pelos seguintes aminoácidos: ácido aspártico (D), histidina (H) e serina (S) (PAGE; CERA, 2008).…”

Section: Análises De Similaridade E Filogenia Da Sequência Lox-c3unclassified

Caracterização e análise filogenética dos genes que codificam para os componentes C3 e fator B do sistema complemento das glândulas de veneno de aranhas Loxosceles.

Myamoto¹

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Evolution of the complement system in protostomes revealed by de novo transcriptome analysis of six species of Arthropoda

Cited by 26 publications

References 42 publications

An updated molecular basis for mussel immunity

An updated molecular basis for mussel immunity

Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly

Caracterização e análise filogenética dos genes que codificam para os componentes C3 e fator B do sistema complemento das glândulas de veneno de aranhas Loxosceles.

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