Microwave line-of-sight radio relay (RR) systems are a constitutive part of a telecom operator transport network, as an alternative to optical transmission systems when the latter are not technically possible or rational to implement. Nowadays, RR links are quite often used in the access network for connecting mobile radio base stations, thus also enabling traffic aggregation, and so on. In this paper, we focus on a practical, real-life, five-section heterogeneous RR network, comprising classic synchronous digital hierarchy (SDH) and SDH new generation network (NGN) architecture, hybrid parallel and mutually independent transmission of native Ethernet and TDM services, and all-IP network parts. Specifically, the main task of this work is to answer whether such a diverse RR system could satisfy the quality norms for Ethernet-based services, meaning whether a tolerable RR unavailability will necessarily imply the according Ethernet quality of service (QoS) degradation. This question is addressed by the comprehensive in-service and out-of-service testing of an operational hybrid RR transmission system. After extensive practical testing and appropriate analysis of the achieved results, it came out that the impact of RR-level impairments that determine the performance prediction affected the Ethernet QoS to the extent that BER values increased to the acceptability threshold values. We believe that the preliminary results reported here could serve as a hint and a framework for a more comprehensive cross-layer test strategy in terms of both test diversity and repeating rate, which contemporary network operators need to implement in order to enable the appropriate quality of experience for users of their services.