Evolution of the parthenogenetic rock lizard hybrid karyotype: Robertsonian translocation between two maternal chromosomes in Darevskia rostombekowi

Spangenberg, Victor; Коломиец, О. Л.; Stepanyan, Ilona; Galoyan, Eduard; Cioffi, Marcelo de Bello; Martynova, Elena; Martirosyan, I. A.; Grishaeva, T. M.; Al-Rikabi, Ahmed; Liehr, Thomas; Arakelyan, Marine

doi:10.1007/s00412-020-00744-7

Cited by 11 publications

(22 citation statements)

References 43 publications

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“…The pair of smallest microchromosomes (m1 and m2) showed the predominant coverage of different colours, red and green. Similar CGH results for the w‐chromosome and microchromosomes were described by us previously in the parthenogenetic species D. rostombekowi (Spangenberg et al, 2020a).…”

Section: Resultssupporting

confidence: 87%

“…Parthenogenetic species D. rostombekowi was result of the ancestral hybridization between D. raddei raddei and D. portschiskii , which has a single submetacentric chromosome. As we previously revealed, this single chromosome was the result of a Robertsonian translocation between two chromosomes of the maternal chromosomal set inherited from D. raddei (Spangenberg et al, 2020a). Robertsonian translocation implies the joining of a pair of acrocentric chromosomes at their centromeres to form a metacentric chromosome (Qumsiyeh, 1994).…”

Section: Discussionmentioning

confidence: 76%

“…The aim of the present study was to reveal if chromosomal rearrangements occurred de novo in parthenogenetic species of the Darevskia genus in addition to the one already known chromosomal translocation described for D. rostombekowi (Spangenberg et al, 2020a). We assumed D. unisexualis found in different geographically isolated populations would be a perfect subject to study.…”

Section: Introductionmentioning

confidence: 96%

“…Parthenogenetic species in the genus Darevskia possess hybrid karyotypes of the same ploidy as parental sexual species 2n = 38. Their karyotype combines two parental chromosomal sets with 19 chromosomes, containing the pericentromeric DNA specific for the maternal species and 19 chromosomes with pericentromeric regions specific for the paternal species (Spangenberg et al, 2020; 2020a).…”

Section: Introductionmentioning

confidence: 99%

“…The combination of different genomes in hybrids was considered as a possible trigger of genomic changes, specifically chromosomal translocations, chromatin elimination, and expansion of transposons (Comai et al, 2003; Rodionov, 2013; Sun et al, 2014). In a previous study, we first identified the transformation of the hybrid karyotype of parthenogenetic species Darevskia rostombekowi by the fusion of two chromosomes leading to a single submetacentric chromosome formation and found that the karyotype of this species was represented by an odd number of chromosomes 2n = 37 (Spangenberg et al, 2020a). As no Rb‐translocations were described in both parental species (Spangenberg et al, 2019), it is more likely that chromosomal fusion occurred de novo in the parthenogenetic species after hybrid karyotype formation.…”

Section: Introductionmentioning

confidence: 99%

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Meiotic synapsis of homeologous chromosomes and mismatch repair protein detection in the parthenogenetic rock lizard Darevskia unisexualis

Spangenberg

Arakelyan

Galoyan

et al. 2021

Molecular Reproduction Devel

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Parthenogenetic species of Caucasian rock lizards of the genus Darevksia are important evidence for reticulate evolution and speciation by hybridization in vertebrates. Female‐only lineages formed through interspecific hybridization have been discovered in many groups. Nevertheless, critical mechanisms of oogenesis and specifics of meiosis that provide long‐term stability of parthenogenetic species are still unknown. Here we report cytogenetic characteristics of somatic karyotypes and meiotic prophase I nuclei in the diploid parthenogenetic species Darevskia unisexualis from the new population “Keti” in Armenia which contains an odd number of chromosomes 2n = 37, instead of the usual 2n = 38. We revealed 36 acrocentric chromosomes and a single metacentric autosomal chromosome, resulting from Robertsonian translocation. Comparative genomic hybridization revealed that chromosome fusion occurred between two chromosomes inherited from the maternal species, similar to another parthenogenetic species D. rostombekowi. To trace the chromosome behaviour in meiosis, we performed an immunocytochemical study of primary oocytes' spread nuclei and studied chromosome synapsis during meiotic prophase I in D. unisexualis based on analysis of synaptonemal complexes (SCs). We found meiotic SC‐trivalent composed of one metacentric and two acrocentric chromosomes. We confirmed that the SC was assembled between homeologous chromosomes inherited from two parental species. Immunostaining of the pachytene and diplotene nuclei revealed a mismatch repair protein MLH1 loaded to all autosomal SC bivalents. Possible mechanisms of meiotic recombination between homeologous chromosomes are discussed.

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Section: Resultssupporting

confidence: 87%

Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Meiotic synapsis of homeologous chromosomes and mismatch repair protein detection in the parthenogenetic rock lizard Darevskia unisexualis

Spangenberg

Arakelyan

Galoyan

et al. 2021

Molecular Reproduction Devel

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Variants of a major DNA satellite discriminate parental subgenomes in a hybrid parthenogenetic lizard Darevskia unisexualis (Darevsky, 1966)

Nikitin,

Sidorov,

Liehr

et al. 2024

J Exp Zool Pt B

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Hybrid parthenogenetic animals are an exceptionally interesting model for studying the mechanisms and evolution of sexual and asexual reproduction. A diploid parthenogenetic lizard Darevskia unisexualis is a result of an ancestral cross between a maternal species Darevskia raddei nairensis and a paternal species Darevskia valentini and presents a unique opportunity for a cytogenetic and computational analysis of a hybrid karyotype. Our previous results demonstrated a significant divergence between the pericentromeric DNA sequences of the parental Darevskia species; however, an in‐depth comparative study of their pericentromeres is still lacking. Here, using target sequencing of microdissected pericentromeric regions, we reveal and compare the repertoires of the pericentromeric tandem repeats of the parental Darevskia lizards. We found species‐specific sequences of the major pericentromeric tandem repeat CLsat, which allowed computational prediction and experimental validation of fluorescent DNA probes discriminating parental chromosomes within the hybrid karyotype of D. unisexualis. Moreover, we have implemented a generalizable computational method, based on the optimization of the Levenshtein distance between tandem repeat monomers, for finding species‐specific fluorescent probes for pericentromere staining. In total, we anticipate that our comparative analysis of Darevskia pericentromeric repeats, the species‐specific fluorescent probes that we found and the pipeline that we developed will form a basis for the future detailed cytogenomic studies of a wide range of natural and laboratory hybrids.

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Integrating Genomic and Cytogenetic Data to Study the Evolutionary History of Arapaimas and Arowanas in the Neotropics

Perez,

Toma,

Souza

et al. 2023

Conservation Genetics in the Neotropics

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Evolution of the parthenogenetic rock lizard hybrid karyotype: Robertsonian translocation between two maternal chromosomes in Darevskia rostombekowi

Cited by 11 publications

References 43 publications

Meiotic synapsis of homeologous chromosomes and mismatch repair protein detection in the parthenogenetic rock lizard Darevskia unisexualis

Meiotic synapsis of homeologous chromosomes and mismatch repair protein detection in the parthenogenetic rock lizard Darevskia unisexualis

Variants of a major DNA satellite discriminate parental subgenomes in a hybrid parthenogenetic lizard Darevskia unisexualis (Darevsky, 1966)

Integrating Genomic and Cytogenetic Data to Study the Evolutionary History of Arapaimas and Arowanas in the Neotropics

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