Evolution of the potassium channel gene Kcnj13 underlies colour pattern diversification in Danio fish

Podobnik, Marco; Frohnhöfer, Hans Georg; Dooley, Christopher M.; Eskova, Anastasia; Nüsslein‐Volhard, Christiane; Irion, Uwe

doi:10.1038/s41467-020-20021-6

“…By contrast, the elimination of iridophores from D. aesculapii had only a limited effect on major pattern features, though mutant individuals tended to be more variable in appearance leading to them occupying a larger area of morphospace than wild-type. The persistence of pattern observed for iridophore-free ltk mutants was also consistent with the phenotype of D. aesculapii that lack iridophores due to mutation at a different locus ( mpv17 ) ( Podobnik et al, 2020 ).…”

Section: Resultssupporting

confidence: 78%

“…Danio pigment patterns offer an outstanding opportunity to learn about the genetic and cellular mechanisms underlying trait evolution, given their largely two-dimensional organization, tractable number of cell types, accessibility to imaging as phenotypes are developing, and approaches available for interrogating developmental genetic mechanisms and modeling pigment cell behaviors ( McCluskey et al, 2021 ; Moreira and Deutsch, 2005 ; Nakamasu et al, 2009 ; Owen et al, 2020 ; Patterson et al, 2014 ; Patterson and Parichy, 2019 ; Podobnik et al, 2020 ; Spiewak et al, 2018 ; Volkening and Sandstede, 2015 , 2018 ; Watanabe and Kondo, 2015b ; Yamanaka and Kondo, 2014 ). Given their developmental origins, these patterns may inform our understanding of species differences in other neural crest derived traits as well.…”

Section: Introductionmentioning

confidence: 99%

Pigment pattern morphospace ofDaniofishes: evolutionary diversification and mutational effects

McCluskey

¹

,

Liang

²

,

Lewis

³

et al. 2021

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Molecular and cellular mechanisms underlying variation in adult form remain largely unknown. Adult pigment patterns of fishes in the genus Danio, which includes zebrafish, D. rerio, consist of horizontal stripes, vertical bars, spots and uniform patterns, and provide an outstanding opportunity to identify causes of species level variation in a neural crest derived trait. Understanding pigment pattern variation requires quantitative approaches to assess phenotypes, yet such methods have been mostly lacking for pigment patterns. We introduce metrics derived from information theory that describe patterns and pattern variation in Danio fishes. We find that these metrics used singly and in multivariate combinations are suitable for distinguishing general pattern types, and can reveal even subtle phenotypic differences attributable to mutations. Our study provides new tools for analyzing pigment pattern in Danio and potentially other groups, and sets the stage for future analyses of pattern morphospace and its mechanistic underpinnings.

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“…In pigeons, coloration has complex epistatic interactions involving three loci [ 78 ] and pattern within individual feathers has an additional, independent genetic component [ 79 ]. By extension, differences in pigment pattern between other pairs of Danio species presumably also have polygenic bases, as has been noted previously [ 4 , 17 , 18 , 80 ]. Though some genes likely contributing to such variation have been identified, additional genes undoubtedly await discovery.…”

Section: Discussionsupporting

confidence: 54%

A complex genetic architecture in zebrafish relatives Danio quagga and D. kyathit underlies development of stripes and spots

McCluskey

¹

,

Uji

²

,

Mancusi

³

et al. 2021

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Vertebrate pigmentation is a fundamentally important, multifaceted phenotype. Zebrafish, Danio rerio, has been a valuable model for understanding genetics and development of pigment pattern formation due to its genetic and experimental tractability, advantages that are shared across several Danio species having a striking array of pigment patterns. Here, we use the sister species D. quagga and D. kyathit, with stripes and spots, respectively, to understand how natural genetic variation impacts phenotypes at cellular and organismal levels. We first show that D. quagga and D. kyathit phenotypes resemble those of wild-type D. rerio and several single locus mutants of D. rerio, respectively, in a morphospace defined by pattern variation along dorsoventral and anteroposterior axes. We then identify differences in patterning at the cellular level between D. quagga and D. kyathit by repeated daily imaging during pattern development and quantitative comparisons of adult phenotypes, revealing that patterns are similar initially but diverge ontogenetically. To assess the genetic architecture of these differences, we employ reduced-representation sequencing of second-generation hybrids. Despite the similarity of D. quagga to D. rerio, and D. kyathit to some D. rerio mutants, our analyses reveal a complex genetic basis for differences between D. quagga and D. kyathit, with several quantitative trait loci contributing to variation in overall pattern and cellular phenotypes, epistatic interactions between loci, and abundant segregating variation within species. Our findings provide a window into the evolutionary genetics of pattern-forming mechanisms in Danio and highlight the complexity of differences that can arise even between sister species. Further studies of natural genetic diversity underlying pattern variation in D. quagga and D. kyathit should provide insights complementary to those from zebrafish mutant phenotypes and more distant species comparisons.

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“…In pigeons, coloration has complex epistatic interactions involving three loci [77] and pattern within individual feathers has an additional, independent genetic component [78]. By extension, differences in pigment pattern between other pairs of Danio species presumably also have polygenic bases, as has been noted previously [4,16,17,79]. Though some genes likely to contributing to such variation have been identified, additional genes undoubtedly await discovery.…”

Section: Discussionmentioning

confidence: 84%

A complex genetic architecture in zebrafish relativesDanio quaggaandD. kyathitunderlies development of stripes and spots

McCluskey

¹

,

Uji

²

,

Mancusi

³

et al. 2021

Preprint

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Vertebrate pigmentation is a fundamentally important, multifaceted phenotype. Zebrafish, Danio rerio, has been a valuable model for understanding genetics and development of pigment pattern formation due to its genetic and experimental tractability, advantages that are shared across several Danio species having a striking array of pigment patterns. Here, we use the sister species D. quagga and D. kyathit, with stripes and spots, respectively, to understand how natural genetic variation impacts phenotypes at cellular and organismal levels. We first show that D. quagga and D. kyathit phenotypes resemble those of wild-type D. rerio and several single locus mutants of D. rerio, respectively, in a morphospace defined by pattern variation along dorsoventral and anteroposterior axes. We then identify differences in patterning at the cellular level between D. quagga and D. kyathit by repeated daily imaging during pattern development and quantitative comparisons of adult phenotypes, revealing that patterns are similar initially but diverge ontogenetically. To assess the genetic architecture of these differences, we employ reduced-representation sequencing of second-generation hybrids. Despite the similarity of D. quagga to D. rerio, and D. kyathit to some D. rerio mutants, our analyses reveal a complex genetic basis for differences between D. quagga and D. kyathit, with several quantitative trait loci contributing to variation in overall pattern and cellular phenotypes, epistatic interactions between loci, and abundant segregating variation within species. Our findings provide a window into the evolutionary genetics of pattern-forming mechanisms in Danio and highlight the complexity of differences that can arise even between sister species. Further studies of natural genetic diversity underlying pattern variation in D. quagga and D. kyathit should provide insights complementary to those from zebrafish mutant phenotypes and more distant species comparisons.Author SummaryPigment patterns of fishes are diverse and function in a wide range of behaviors. Common pattern themes include stripes and spots, exemplified by the closely related minnows Danio quagga and D. kyathit, respectively. We show that these patterns arise late in development owing to alterations in the development and arrangements of pigment cells. In the closely related model organism zebrafish (D. rerio) single genes can switch the pattern from stripes to spots. Yet, we show that pattern differences between D. quagga and D. kyathit have a more complex genetic basis, depending on multiple genes and interactions between these genes. Our findings illustrate the importance of characterizing naturally occuring genetic variants, in addition to laboratory induced mutations, for a more complete understanding of pigment pattern development and evolution.

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Evolution of the potassium channel gene Kcnj13 underlies colour pattern diversification in Danio fish

Cited by 23 publications

References 91 publications

Pigment pattern morphospace ofDaniofishes: evolutionary diversification and mutational effects

Pigment pattern morphospace ofDaniofishes: evolutionary diversification and mutational effects

A complex genetic architecture in zebrafish relatives Danio quagga and D. kyathit underlies development of stripes and spots

A complex genetic architecture in zebrafish relativesDanio quaggaandD. kyathitunderlies development of stripes and spots

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