Evolution of the Terminal Regions of the Streptomyces Linear Chromosome

Choulet, Frédéric; Aigle, Bertrand; Gallois, Alexandre; Mangenot, Sophie; Gerbaud, Claude; Truong, Chantal; Francou, François‐Xavier; Fourrier, Céline; Guérineau, Michel; Decaris, Bernard; Barbe, Valérie; Pernodet, Jean‐Luc; Leblond, Pierre

doi:10.1093/molbev/msl108

Cited by 89 publications

(106 citation statements)

References 53 publications

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“…Interestingly, AICESco5349 flanks the whiE cluster (Bentley et al, 2002) encoding the enzymes to synthesise the grey polyketide spore pigment (Davis and Chater, 1990) and AICESco3250 flanks the calcium-dependent antibiotic (CDA) biosynthetic gene cluster. The two AICEs are absent on the highly syntenic core region of the S. lividans genome (Jayapal et al, 2007) and the S. ambofaciens genome lacks the CDA cluster and also the complete region containing the whiE cluster and AICESco5349 (Choulet et al, 2006). Possibly, these AICEs are associated with the acquisition of these secondary metabolite clusters in S. coelicolor.…”

Section: Discussionmentioning

confidence: 99%

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

Poele

Samborskyy

Oliynyk

et al. 2008

Plasmid

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Section: Discussionmentioning

confidence: 99%

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

Poele

Samborskyy

Oliynyk

et al. 2008

Plasmid

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“…It has been reported that telomeric regions in S. ambofaciens are frequently deleted by induction of mutagenesis (38). Analysis revealed that monoterpene cyclase (SAML0357) and methyltransferase (SAML0358) genes were located near the left telomere.…”

Section: Production Of Volatile Terpenoid Metabolites In Actinomycetementioning

confidence: 99%

Identification and functional analysis of genes controlling biosynthesis of 2-methylisoborneol

Komatsu

Tsuda

Ōmura

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

224

210

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To identify the genes for biosynthesis of the off-flavor terpenoid alcohol, 2-methylisoborneol (2-MIB), the key genes encoding monoterpene cyclase were located in bacterial genome databases by using a combination of hidden Markov models, protein-family search, and the sequence alignment of their gene products. Predicted terpene cyclases were classified into three groups: sesquiterpene, diterpene, and other terpene cyclases. Genes of the terpene cyclase group that form an operon with a gene encoding S-adenosyl-L-methionine (SAM)-dependent methyltransferase were found in genome data of seven microorganisms belonging to actinomycetes, Streptomyces ambofaciens ISP5053, Streptomyces coelicolor A3(2), Streptomyces griseus IFO13350, Streptomyces lasaliensis NRRL3382R, Streptomyces scabies 87.22, Saccharopolyspora erythraea NRRL2338, and Micromonospora olivasterospora KY11048. Among six microorganisms tested, S. ambofaciens, S. coelicolor A3(2), S. griseus, and S. lasaliensis produced 2-MIB but M. olivasterospora produced 2-methylenebornane (2-MB) instead. The regions containing monoterpene cyclase and methyltransferase genes were amplified by PCR from S. ambofaciens, S. lasaliensis, and Saccharopolyspora erythraea, respectively, and their genes were heterologously expressed in Streptomyces avermitilis, which was naturally deficient of 2-MIB biosynthesis by insertion and deletion. All exoconjugants of S. avermitilis produced 2-MIB. Full-length recombinant proteins, monoterpene cyclase and methyltransferase of S. lasaliensis were expressed at high level in Escherichia coli. The recombinant methyltransferase catalyzed methylation at the C2 position of geranyl diphosphate (GPP) in the presence of SAM. 2-MIB was generated by incubation with GPP, SAM, recombinant methyltransferase, and terpene cyclase. We concluded that the biosynthetic pathway involves the methylation of GPP by GPP methyltransferase and its subsequent cyclization by monoterpene cyclase to 2-MIB.genome mining ͉ methyltransferase ͉ monoterpene cyclase T erpenoid metabolites, monoterpenes, sesquiterpenes, and diterpenes, have been isolated from terrestrial and marine plants or from fungi, with only a relatively minor fraction from prokaryotes. Their compounds are used as antibiotics, hormones, flavor or odor constituents, and pigments. Some of them possess other physiologically or commercially important properties (1, 2). Three terpenoid compounds (Fig. 1), 2-methylisoborneol (2-MIB), geosmin, and albaflavenone are known as odorous and volatile microbial metabolites. The former two terpenoid alcohols are the most frequently found secondary metabolites of actinomycetes (3-5), filamentous cyanobacteria (6-8), myxobacteria (9), and fungi (10, 11), and account for many odor problems encountered with freshwater or with fish (7,(12)(13)(14). Geosmin is also known to contribute to the characteristic earthy red beet flavor (15). 2-MIB is related to the musty-earthy notes in Brie and Camembert cheese flavor (16). An ␣,␤-unsaturated sesquiterpene ketone, albaflavenone, ...

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“…AM287205). Moreover, as the synteny is strong in the central region of the S. coelicolor and S. ambofaciens (Choulet et al, 2006), the other des genes are likely to be conserved as well and present in the same chromosomal region. Analysis and annotation of the chromosome arm sequences identified a cluster in the right arm virtually identical (80-94 % identity at the protein level) to the cch cluster found in S. coelicolor (coding sequences SAMR0548 to SAMR0559, accession no.…”

Section: Conservation Of the Cch And Des Clusters In S Ambofaciensmentioning

confidence: 99%

“…The sequence of about 1?4 Mb of each chromosome arm of S. ambofaciens ATCC 23877 has been determined (Choulet et al, 2006). In addition, the insert extremities of about 5000 clones from a BAC library of the S. ambofaciens chromosome have been sequenced, leading to 40 % coverage of the complete chromosome and providing some information on the genes present in the central part of the chromosome.…”

Section: Conservation Of the Cch And Des Clusters In S Ambofaciensmentioning

confidence: 99%

Multiple biosynthetic and uptake systems mediate siderophore-dependent iron acquisition in Streptomyces coelicolor A3(2) and Streptomyces ambofaciens ATCC 23877

et al. 2006

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Siderophore-mediated iron acquisition has been well studied in many bacterial pathogens because it contributes to virulence. In contrast, siderophore-mediated iron acquisition by saprophytic bacteria has received relatively little attention. The independent identification of the des and cch gene clusters that direct production of the tris-hydroxamate ferric iron-chelators desferrioxamine E and coelichelin, respectively, which could potentially act as siderophores in the saprophyte Streptomyces coelicolor A3(2), has recently been reported. Here it is shown that the des cluster also directs production of desferrioxamine B in S. coelicolor and that very similar des and cch clusters direct production of desferrioxamines E and B, and coelichelin, respectively, in Streptomyces ambofaciens ATCC 23877. Sequence analyses of the des and cch clusters suggest that components of ferric-siderophore uptake systems are also encoded within each cluster. The construction and analysis of a series of mutants of S. coelicolor lacking just biosynthetic genes or both the biosynthetic and siderophore uptake genes from the des and cch clusters demonstrated that coelichelin and desferrioxamines E and B all function as siderophores in this organism and that at least one of these metabolites is required for growth under defined conditions even in the presence of significant quantities of ferric iron. These experiments also demonstrated that a third siderophore uptake system must be present in S. coelicolor, in addition to the two encoded within the cch and des clusters, which show selectivity for coelichelin and desferrioxamine E, respectively. The ability of the S. coelicolor mutants to utilize a range of exogenous xenosiderophores for iron acquisition was also examined, showing that the third siderophore-iron transport system has broad specificity for tris-hydroxamate-containing siderophores. Together, these results define a complex system of multiple biosynthetic and uptake pathways for siderophore-mediated iron acquisition in S. coelicolor and S. ambofaciens.

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Evolution of the Terminal Regions of the Streptomyces Linear Chromosome

Cited by 89 publications

References 53 publications

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

Identification and functional analysis of genes controlling biosynthesis of 2-methylisoborneol

Multiple biosynthetic and uptake systems mediate siderophore-dependent iron acquisition in Streptomyces coelicolor A3(2) and Streptomyces ambofaciens ATCC 23877

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